Microbiome-based screening and co-fermentation of rhizospheric microorganisms for highly ginsenoside Rg3 production

Qi, Geyuan; Ji, Baoyu; Zhang, Yanan; Huang, Luqi; Wang, Juan; Gao, Wenyuan

doi:10.1016/j.micres.2022.127054

Cited by 2 publications

(2 citation statements)

References 37 publications

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“…Amount. Te initial glycogen amount will afect the concentration of bacteria in the fermentation broth during the stable period of the strain, thereby afecting the enzymeproducing activity of the strain, which in turn will afect the conversion rate of saponins [24]. Determining the proportion of glycogen can not only maximize the conversion rate of saponins after fermentation but also reduce the sugar content in ginseng jiaosu after fermentation, thereby increasing the categories of ginseng jiaosu benefciaries.…”

Section: Experiments For the Determination Of Glycogen Additionmentioning

confidence: 99%

Optimization of Rare Ginsenosides and Antioxidant Activity Quality of Ginseng Jiaosu Based on Probiotic Strains and Fermentation Technology

Tang

Liu

et al. 2023

Journal of Food Quality

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The primary active components of plant ginseng are saponins, and rare saponins play a specific antitumor action. In this study, Lactobacillus that can efficiently transform rare saponins were screened as fermentation strains of ginseng enzymes. The fermentation process was optimized to investigate the changes in various biochemical indicators and antioxidant activities of the enzymes before and after fermentation. The results showed that Lactiplantibacillus plantarum had the highest conversion efficiency for diol saponins, among which the content of rare ginsenoside-CK increased by 256%. The optimum optimizing method was a 1 : 1 : 10 ratio of ginseng extract/concentrated apple juice/water, fermentation time of 16 days, initial pH of 6.0, fermentation temperature of 37°C, and sterilization amount of 1.0%. The contents of rare ginsenoside-Rh1, ginsenoside-F2, ginsenoside-Rg3, and ginsenoside-CK increased by 18.48%, 135.73%, 343.03%, and 441.80%, respectively, and the process was stable and controllable. After fermentation, the scavenging rates for hydroxyl radical, DPPH radical, and superoxide anion radical were increased by more than 9%, and new organic acids were produced. In general, this study established an efficient, stable, and controllable ginseng fermentation process to improve the rare saponin content and biological activity of ginseng enzymes, which can provide new ideas for traditional Chinese medicine and medicinal health food in China.

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Section: Experiments For the Determination Of Glycogen Additionmentioning

confidence: 99%

Optimization of Rare Ginsenosides and Antioxidant Activity Quality of Ginseng Jiaosu Based on Probiotic Strains and Fermentation Technology

Tang

Liu

et al. 2023

Journal of Food Quality

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“…Since Rb1 is the most abundant compound in ginseng, it is frequently employed as the primary substrate and initiator in bioconversion reactions. The Rb1 molecule consists of two glucose molecules at the C3 position and two more at the C20 position; these glucose moieties are readily cleaved to produce intermediates or minor target compounds ( Table 1 and Table 2 ) [ 2 , 6 , 8 , 9 , 49 , 50 , 51 , 52 , 53 , 54 , 55 ]. Ginsenoside bioconversion is illustrated in Figure 2 .…”

Section: Bioconversion Of Ginsenosidesmentioning

confidence: 99%

β-Glucosidase and Its Application in Bioconversion of Ginsenosides in Panax ginseng

et al. 2023

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Ginsenosides are a group of bioactive compounds isolated from Panax ginseng. Conventional major ginsenosides have a long history of use in traditional medicine for both illness prevention and therapy. Bioconversion processes have the potential to create new and valuable products in pharmaceutical and biological activities, making them both critical for research and highly economic to implement. This has led to an increase in the number of studies that use major ginsenosides as a precursor to generate minor ones using β-glucosidase. Minor ginsenosides may also have useful properties but are difficult to isolate from raw ginseng because of their scarcity. Bioconversion processes have the potential to create novel minor ginsenosides from the more abundant major ginsenoside precursors in a cost-effective manner. While numerous bioconversion techniques have been developed, an increasing number of studies have reported that β-glucosidase can effectively and specifically generate minor ginsenosides. This paper summarizes the probable bioconversion mechanisms of two protopanaxadiol (PPD) and protopanaxatriol (PPT) types. Other high-efficiency and high-value bioconversion processes using complete proteins isolated from bacterial biomass or recombinant enzymes are also discussed in this article. This paper also discusses the various conversion and analysis methods and their potential applications. Overall, this paper offers theoretical and technical foundations for future studies that will be both scientifically and economically significant.

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Microbiome-based screening and co-fermentation of rhizospheric microorganisms for highly ginsenoside Rg3 production

Cited by 2 publications

References 37 publications

Optimization of Rare Ginsenosides and Antioxidant Activity Quality of Ginseng Jiaosu Based on Probiotic Strains and Fermentation Technology

Optimization of Rare Ginsenosides and Antioxidant Activity Quality of Ginseng Jiaosu Based on Probiotic Strains and Fermentation Technology

β-Glucosidase and Its Application in Bioconversion of Ginsenosides in Panax ginseng

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