Microbiological dynamics of red complex bacteria following full-mouth air polishing in periodontally healthy subjects—a randomized clinical pilot study

Reinhardt, Belinda; Klocke, Astrid; Neering, Sarah H; Selbach, Sabine; Peters, Ulrike; Flemmig, Thomas Frank; Beikler, Thomas

doi:10.1007/s00784-019-02821-3

Cited by 20 publications

(11 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Both US and AP demonstrated decrease of the proportion of pathogenic microbiota and increase of the proportion of commensal microbiota after treatment, which was consistent with previous studies tested by checkerboard DNA–DNA hybridization (Kargas, Tsalikis, Sakellari, Menexes, & Konstantinidis, ; Wennstrom et al, ). Bacteria recolonized after treatment and returned to the pre‐treatment level 12 weeks after treatment, which is similar to that reported before (Lu et al, ; Reinhardt et al, ). As for metabolic pathways, the advanced bacterial activities and pathogenic functions also have significantly decreased after maintenance treatment, while relative abundance of basic cell mobility increased which demonstrated less pathogenic to periodontal tissue.…”

Section: Discussionsupporting

confidence: 89%

Microbiome in maintained periodontitis and its shift over a single maintenance interval of 3 months

Zhao

Feng

et al. 2019

J Clinic Periodontology

View full text Add to dashboard Cite

Aim To assess the subgingival microbial shift of maintained periodontitis treated by ultrasonic scaling (US) or air polishing (AP) during a 3‐month maintenance interval. Materials and methods We conducted a 12‐week randomized split‐mouth controlled trial with US and AP in 17 maintained subjects (bleeding on probing [BOP%] ≤25%, probing depth [PD] ≤5 mm). They were monitored at baseline, week 2, week 8 and week 12. The V3‐V4 region of the 16S rDNA from 136 subgingival plaque samples was sequenced and analysed. Results Treatment by US or AP could effectively reduce the PD, microbial richness, diversity, periodontitis‐associated microbiota and pathogenic metabolism in maintained periodontitis. Bacteria recolonized after treatment and returned to the pre‐treatment level 12 weeks after treatment. Ultrasonic scaling group demonstrated slight advantage in reducing BOP (%), pathogenic bacteria and metabolism than AP group. Pathogenic microbiota and commensal microbiota kept a balance in subgingival community of maintained patients during the 3‐month interval. Conclusions Treatment by US or AP effectively reduced the pathogenicity of subgingival microbiome by reducing microbial diversity, proportion of periodontitis‐associated microbiota and pathogenic metabolism. It helped to keep a balanced subgingival community and stable periodontal condition over a single maintenance interval of 3 months.

show abstract

Section: Discussionsupporting

confidence: 89%

Microbiome in maintained periodontitis and its shift over a single maintenance interval of 3 months

Zhao

Feng

et al. 2019

J Clinic Periodontology

View full text Add to dashboard Cite

show abstract

“…The only study using air-polishing with erythritol as an adjunct, found decreased P. gingivalis counts at one month, but there was only a follow-up to 3 months [14]. Fullmouth treatment of systemically and periodontally healthy individuals with air-polishing with glycin powder revealed a signi cant decrease of bacteria being associated with periodontal disease up to 9 days following intervention, however the counts returned to baseline after 6 weeks [43]. In the present study, the combination of using rst subgingivally air-polishing with erythritol and after three months supragingivally may lead to the bene cial results regarding the number of sites with PD ≥ 5mm which seemed to be associated with reduction of T. forsythia and T. denticola.…”

Section: Discussionmentioning

confidence: 99%

Adjunctive air-polishing with erythritol in nonsurgical periodontal therapy – a randomized clinical trial

Jentsch

Flechsig

Kette

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: This study was aimed to investigate if the adjunctive use of erythritol air-polishing powder applied with the nozzle-system during subgingival instrumentation (SI) has an effect on the outcome of non-surgical periodontal treatment in patients with moderate to severe periodontitis. Methods: Fourty-two individuals with periodontitis received nonsurgical periodontal therapy by SI without (controls, n=21) and with adjunctive air-polishing using nozzle + erythritol powder (test, n=21). They were analyzed for the clinical variables BOP (primary outcome at six month), probing depth (PD), attachment level (AL), four selected microorganisms and two biomarkers at baseline, before SI as well as three and six months after SI. Statistical analysis included nonparametric tests for intra- and intergroup comparisons. Results: In both groups, the clinical variables probing depth (PD), attachment level and BOP significantly improved three and six months after SI. The number of sites with PD ≥ 5mm was significantly lower in the test group than in the control group after six months. At six months vs. baseline, there were significant reductions of Tannerella forsythia and Treponema denticola counts as well as lower levels of MMP-8 in the test group. Conclusions: Subgingival instrumentation with adjunctive erythritol air-polishing powder does not reduce BOP. But it may add beneficial effects like reducing the probing depth measured as number of residual periodontal pocket with PD ≥ 5mm when compared with subgingival instrumentation only. Clinical relevance: The adjunctive use of erythritol air-polishing powder applied with the nozzle-system during SI may improve the clinical outcome of SI and may reduce the need for periodontal surgery. Trial registration: The study was retrospectively registered in the German register of clinical trials, DRKS00015239 on 6th August 2018, https://www.drks.de/drks_web/navigate.do?navigationId =trial.HTML&TRIAL

show abstract

“…For Tannerella forsythia (T. forsythia) however, bacterial counts were favorable for airpolishing after ten days returning to baseline values after 90 days [11]. Also, in patients without periodontitis, an adjunctive supragingival full-mouth-disinfection by airpolishing could lower counts of red complex bacteria like P. gingivalis and T. forsythia after nine days, but returning to baseline values after six and twelve weeks [12]. In a consensus conference in 2017 it was summarized, that subgingival air-polishing in periodontal pocket depths up to 9 mm is more effective in the removal of biofilm than hand instrumentation or (ultra-) sonic scalers [13].…”

Section: Introductionmentioning

confidence: 99%

Effects of subgingival air-polishing with trehalose powder on oral biofilm during periodontal maintenance therapy: a randomized-controlled pilot study

et al. 2020

View full text Add to dashboard Cite

Background: This pilot study was part of a larger study which compared the effect of subgingival air-polishing using trehalose powder with sonic scaling on clinical parameters during supportive periodontal therapy. Within this microbiological part of the investigation subgingival samples were taken from 10 participants to analyze the survival of different bacterial species after the two different treatments as a proof of principle. Methods: In 10 participants two non-adjacent, single-root teeth requiring treatment (PD =5 mm with bleeding on probing (BOP) or > 5 mm) were selected following a split-mouth design and were treated either with a sonic scaler or air-polishing device and trehalose powder. For persistent pockets (PD =4 mm and BOP or > 4 mm), treatment was repeated after 3 months. Subgingival biofilm samples were taken at baseline (BL), subsequently and three and six months after treatment. After determination of the bacterial counts (TBL), isolated bacteria were identified by MALDI-TOF-MS. If unsuccessful, PCR and 16S rDNA sequencing were performed. Results: In both treatment groups, TBL decreased immediately after treatment remaining at a lower level. This confirms the findings of the larger study regarding clinical parameters showing a comparable effect on PD, BOP and CAL. Immediately after treatment, the diversity of detected species decreased significantly more than in the sonic group (p = 0.03). After 3 months, the proportion of Gram-positive anaerobic rods was lower in the airpolishing group (powder/ sonic 7%/ 25.9%, p = 0.025). Also, there was a greater reduction of Gram-negative aerobic rods for this group at this time (air-polishing/ sonic − 0.91 /-0.23 Log10 cfu/ ml, p = 0.020). Conclusion: Within the limitations of this study air-polishing and sonic treatment seem to have a comparable effect on the subgingival oral biofilm during supportive periodontal treatment. Trial registration: The study was registered in an international trial register (German Clinical Trial Register number DRKS 00006296) on 10th of June 2015. HTML&TRIAL_ID = DRKS00006296.

show abstract

Microbiological dynamics of red complex bacteria following full-mouth air polishing in periodontally healthy subjects—a randomized clinical pilot study

Cited by 20 publications

References 57 publications

Microbiome in maintained periodontitis and its shift over a single maintenance interval of 3 months

Microbiome in maintained periodontitis and its shift over a single maintenance interval of 3 months

Adjunctive air-polishing with erythritol in nonsurgical periodontal therapy – a randomized clinical trial

Effects of subgingival air-polishing with trehalose powder on oral biofilm during periodontal maintenance therapy: a randomized-controlled pilot study

Contact Info

Product

Resources

About