Microbial spermidine dehydrogenase: Purification and properties of the enzyme in Pseudomonas aeruginosa and Citrobacter freundii

Characterization and a role of Pseudomonas aeruginosa spermidine dehydrogenase in polyamine catabolism

“…However, the impaired growth of spuA and spuB mutants on spermidine and spermine suggests that the SpuA and SpuB enzymes play major roles in the catabolism of these polyamines. In addition, P. aeruginosa expresses spermidine dehydrogenase (SpdH; EC 1.5.99.6), which has also been identified in Citrobacter freundii and Serratia marcesens (Hisano et al, 1990(Hisano et al, , 1992aTabor & Kellogg, 1970). The SpdH of P. aeruginosa IFO3080 is constitutively expressed and has been purified as a 63 kDa monomer, but it remains to be characterized in detail (Hisano et al, 1990).…”

“…In addition, P. aeruginosa expresses spermidine dehydrogenase (SpdH; EC 1.5.99.6), which has also been identified in Citrobacter freundii and Serratia marcesens (Hisano et al, 1990(Hisano et al, , 1992aTabor & Kellogg, 1970). The SpdH of P. aeruginosa IFO3080 is constitutively expressed and has been purified as a 63 kDa monomer, but it remains to be characterized in detail (Hisano et al, 1990). In contrast, C. freundii and S. marcesens SpdHs are haem proteins that are inducible by spermidine and they both cleave spermidine to yield 1,3-diaminopropane and 4-aminobutyraldehyde ( Fig.…”

Further Properties of Spermidine Dehydrogenase from Citrobacter freundii IFO 12681

“…The bacterial strain used and culture conditions were the same as reported previously. 8) Assays. The assay of spermidine dehydrogenase was done by the method described previously8) with some modifications.…”

Occurrence and enzymic degradation of glutathionylspermidine in Citrobacter freundii

“…The activity requiring componwith those of reactions catalysed by crude cell extracts ents in f2 and f3 to produce PI * and P3* was designated and SPD dehydrogenase, which we call E 1, purified from E3 and that responsible for the formation of P2 in cell C. freundii cells grown in Vogel-Bonner minimal extracts was designated E2. medium supplemented with SPD (Hisano et al, 1990) ( Fig. 3).…”

“…These observations on the occurrence of a conjugate between GSH and SPD suggest an additional function of GSH and SPD. In order to learn more about the physiological function of SPD, we initiated the study on the metabolic fate of SPD in a bacterium, Citrobacter jreundii IF0 12681, and reported the detailed properties of SPD dehydrogenase in the bacterium (Hisano et al, 1990). This report describes the occurrence of GSH-SPD in C. jreundii cells and demonstrates the presence of enzymes responsible for its degradation.…”