Microbial siderophore – A boon to agricultural sciences

Ghosh, S.; Bera, Tanmay; Chakrabarty, Ananda M.

doi:10.1016/j.biocontrol.2020.104214

Cited by 93 publications

(45 citation statements)

References 159 publications

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“…Microbes that could release siderophores scavenge the iron molecules from the soil leading to iron starvation and death of other competing counterparts (Leong 1986). Siderophore-producing microbes act as biocontrol agents by inhibiting the growth of soil-borne pathogens (Ghosh et al 2020). Microbial siderophores also induce plant immunity making their host plant disease-resistant (Aznar et al 2014).…”

Section: Resultsmentioning

confidence: 99%

Rhizospheric and endophyticPseudomonas aeruginosain edible vegetable plants share molecular and metabolic traits with clinical isolates

Ambreetha

Marimuthu

Mathee

et al. 2021

Preprint

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Pseudomonas aeruginosa, a leading opportunistic pathogen causing hospital-acquired infections is predominantly present in agricultural settings. There are minimal attempts to examine the molecular and functional attributes shared by agricultural and clinical strains of P. aeruginosa. This study aims to investigate the presence of P. aeruginosa in edible vegetable plants (including salad vegetables) and analyze the evolutionary and metabolic relatedness of the agricultural and clinical strains. Eighteen rhizospheric and endophytic P. aeruginosa strains were isolated from cucumber, tomato, eggplant, and chili directly from the farms. The identity of these strains was confirmed using biochemical, and molecular markers and their genetic and metabolic traits were compared with clinical isolates. DNA fingerprinting analyses and 16S rDNA-based phylogenetic tree revealed that the plant- and human-associated strains are evolutionarily related. Both agricultural and clinical isolates possessed plant-beneficial properties, including mineral solubilization (phosphorous, potassium, and zinc), ammonification, and the ability to release extracellular siderophore and indole-3 acetic acid. These findings suggest that rhizospheric and endophytic P. aeruginosa strains are genetically and functionally analogous to the clinical isolates. This study highlights the edible plants as a potential source for human and animal transmission of P. aeruginosa.

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Section: Resultsmentioning

confidence: 99%

Rhizospheric and endophyticPseudomonas aeruginosain edible vegetable plants share molecular and metabolic traits with clinical isolates

Ambreetha

Marimuthu

Mathee

et al. 2021

Preprint

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“…Thus, siderophore production by PGPR gives them competitive advantages in colonizing roots and in excluding pathogenic microbes from rhizosphere ecological sites [ 30 ]. Different categories of bacterial siderophores have been identified and mainly include hydroxamates, carboxylates, and catecholates [ 31 ], and they show varied abilities to sequestrate iron in vitro . In general, they have higher affinity for ferric iron, particularly those produced by fluorescent Pseudomonas , compared to the fungal siderophores [ 29 , 32 ].…”

Section: Mechanisms Of Pgpr In Disease Suppressionmentioning

confidence: 99%

Pathogen Biocontrol Using Plant Growth-Promoting Bacteria (PGPR): Role of Bacterial Diversity

et al. 2021

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A vast microbial community inhabits in the rhizosphere, among which, specialized bacteria known as Plant Growth-Promoting Rhizobacteria (PGPR) confer benefits to host plants including growth promotion and disease suppression. PGPR taxa vary in the ways whereby they curtail the negative effects of invading plant pathogens. However, a cumulative or synergistic effect does not always ensue when a bacterial consortium is used. In this review, we reassess the disease-suppressive mechanisms of PGPR and present explanations and illustrations for functional diversity and/or stability among PGPR taxa regarding these mechanisms. We also provide evidence of benefits when PGPR mixtures, rather than individuals, are used for protecting crops from various diseases, and underscore the critical determinant factors for successful use of PGPR mixtures. Then, we evaluate the challenges of and limitations to achieving the desired outcomes from strain/species-rich bacterial assemblages, particularly in relation to their role for plant disease management. In addition, towards locating additive or synergistic outcomes, we highlight why and how the benefits conferred need to be categorized and quantified when different strains/species of PGPR are used in combinations. Finally, we highlight the critical approaches needed for developing PGPR mixtures with improved efficacy and stability as biocontrols for utilization in agricultural fields.

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“…For instance, van Loon et al (2008) showed that the production of siderophores by endophytes contributes to protecting the host plant through induced systemic resistance (ISR), a mechanism in the plant activated by an infection. Furthermore, siderophores are considered potential biocontrol agents; endophytic siderophore production reduces the amount of ferric ions available for uptake by pathogens, contributing to the plant defense (Ghosh et al, 2020;Kramer et al, 2020). Endophytic S. pulveraceus ES16 can produce the rare iron-chelating siderophore mirubactin (compound 8, Figure 3), which it also secretes out as revealed by MALDI-HRMSI.…”

Section: Production Of Bioactive Ptms and The Siderophore Mirubactin mentioning

confidence: 99%

“…Thirdly, mirubactin (8) was also secreted by the endophyte, as evidenced by the MALDI-HRMSI, similar to the PTMs (2-7). Therefore, it can be anticipated that this siderophore is also released into the plant apoplast by endophytic S. pulveraceus ES16 and can add to the PTMs-mediated "warfare" against various microorganisms residing in the immediate environment by chelating ferric ions available for uptake by associated or invading microorganisms (Ghosh et al, 2020;Kramer et al, 2020).…”

Section: Insights Gained From the Chemical Evaluation Of Endophytic Smentioning

confidence: 99%

Evaluation of Apple Root-Associated Endophytic Streptomyces pulveraceus Strain ES16 by an OSMAC-Assisted Metabolomics Approach

Armin

Zühlke

Mahnkopp-Dirks

et al. 2021

Front. Sustain. Food Syst.

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The One Strain Many Compounds approach (OSMAC) is a powerful and comprehensive method that enables the chemo-diversity evaluation of microorganisms. This is achieved by variations of physicochemical cultivation parameters and by providing biotic and abiotic triggers to mimic microorganisms' natural environment in the lab. This approach can reactivate the silent biosynthetic routes of specific metabolites typically not biosynthesized under standard laboratory conditions. In the present study, we combined the OSMAC approach with static headspace solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS), high-performance liquid chromatography-high-resolution tandem mass spectrometry (HPLC-HRMSn), and matrix-assisted laser desorption/ionization high-resolution mass spectrometry imaging (MALDI-HRMSI) to evaluate the chemoecological significance of an apple root-associated endophytic Streptomyces pulveraceus strain ES16. We employed the OSMAC approach by cultivating the endophyte in six different media conditions and performed temporal studies over 14 days. Analysis of the volatilome revealed that only under stressful conditions associated with sporulation, endophytic S. pulveraceus ES16 produces geosmin, a volatile semiochemical known to attract the soil arthropods Collembola (springtails) specifically. Subsequently, targeted metabolic profiling revealed polycyclic tetramate macrolactams (PTMs) production by the endophyte under stress, which are bioactive against various pathogens. Additionally, the endophyte produced the iron-chelating siderophore, mirubactin, under the same conditions. The structures of the compounds were evaluated using HRMSn and by comparison with literature data. Finally, MALDI-HRMSI revealed the produced compounds' spatial-temporal distribution over 14 days. The compounds were profusely secreted into the medium after production. Our results indicate that endophytic S. pulveraceus ES16 can release the signal molecule geosmin, chemical defense compounds such as the PTMs, as well as the siderophore mirubactin into the host plant apoplast or the soil for ecologically meaningful purposes, which are discussed.

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Microbial siderophore – A boon to agricultural sciences

Cited by 93 publications

References 159 publications

Rhizospheric and endophyticPseudomonas aeruginosain edible vegetable plants share molecular and metabolic traits with clinical isolates

Rhizospheric and endophyticPseudomonas aeruginosain edible vegetable plants share molecular and metabolic traits with clinical isolates

Pathogen Biocontrol Using Plant Growth-Promoting Bacteria (PGPR): Role of Bacterial Diversity

Evaluation of Apple Root-Associated Endophytic Streptomyces pulveraceus Strain ES16 by an OSMAC-Assisted Metabolomics Approach

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