Microbial production of polyhydroxyalkanoates (PHAs) and its copolymers: A review of recent advancements

Anjum, Anbreen; Zuber, Mohammad; Zia, Khalid Mahmood; Noreen, Aqdas; Anjum, Muhammad Naveed; Tabasum, Shazia

doi:10.1016/j.ijbiomac.2016.04.069

Cited by 537 publications

(354 citation statements)

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“…Initial elastic response is followed by a large deformation (> 500%) before failure. This is around half the strain reported for bulk materials of similar composition (Saito and Doi, 1994;Anjum et al, 2016). This is in agreement with literature which suggests that the electrospinning leads to reduced strain before failure from a combination of lower density of the material, pre-existing molecular alignment, and higher stresses experienced by the fibers from stress concentrations and early strain hardening (Pedicini and Farris, 2003;Zussman et al, 2003).…”

Section: Resultssupporting

confidence: 91%

“…Poly (3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB)) is a type of polyester in the family of polyhydroxyalkanoates (PHA) and is produced by microorganisms under unbalanced growth conditions (Türesin et al, 2001). PHAs are of significant interest to the biomedical community due to their biodegradability, biocompatibility, and mechanical properties (Anjum et al, 2016;Li and Loh, 2017). Physical properties of microbial polyesters can be regulated by varying the proportions of the copolymers.…”

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confidence: 99%

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X-ray Tomographic Imaging of Tensile Deformation Modes of Electrospun Biodegradable Polyester Fibers

et al. 2017

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Electrospinning allows the production of fibrous networks for tissue engineering, drug delivery, and wound healing in health care. It enables the production of constructs with large surface area and a fibrous morphology that closely resembles the extracellular matrix of many tissues. A fibrous structure not only promotes cell attachment and tissue formation but could also lead to very interesting mechanical properties. Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB)) is a biodegradable polyester that exhibits a large (>400%) elongation before failure. In this study, synchrotron X-ray phase contrast imaging was performed during tensile deformation to failure on a non-woven fiber mat of P(3HB-co-4HB) fibers. Significant reorientation of the fibers in the straining direction was observed, followed by localized necking and eventual failure. From an original average fiber diameter of 4.3 µm, a bimodal distribution of fiber diameter (modal diameters of 1.9 and 3.7 µm) formed after tensile deformation. Extensive localized necking (thinning) of fibers between (thicker) fiber-fiber contacts was found to be the cause for non-uniform thinning of the fibers, a phenomenon that is expected but has not been observed in 3D previously. The data presented here have implications not only in tissue regeneration but for fibrous materials in general.

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Section: Resultssupporting

confidence: 91%

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confidence: 99%

X-ray Tomographic Imaging of Tensile Deformation Modes of Electrospun Biodegradable Polyester Fibers

et al. 2017

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“…These biopolymers continue to attract increasing industrial interest as a renewable, biodegradable, biocompatible thermoplastics being considered as a replacement for hazardous petrochemical polymers. Several reviews have described their potential applications [2, 6, 7]. The main groups of PHAs are short-chain-length PHAs (scl-PHAs) with five or less carbon atoms, and medium-chain-length PHAs (mcl-PHAs) with six or more carbon atoms.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome remodeling of Pseudomonas putida KT2440 during mcl-PHAs synthesis: effect of different carbon sources and response to nitrogen stress

Możejko-Ciesielska

Pokój

Ciesielski

2018

Journal of Industrial Microbiology and Biotechnology

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Bacterial response to environmental stimuli is essential for survival. In response to fluctuating environmental conditions, the physiological status of bacteria can change due to the actions of transcriptional regulatory machinery. The synthesis and accumulation of polyhydroxyalkanoates (PHAs) are one of the survival strategies in harsh environments. In this study, we used transcriptome analysis of Pseudomonas putida KT2440 to gain a genome-wide view of the mechanisms of environmental-friendly biopolymers accumulation under nitrogen-limiting conditions during conversion of metabolically different carbon sources (sodium gluconate and oleic acid). Transcriptomic data revealed that phaG expression is associated with medium-chain-length-PHAs’ synthesis not only on sodium gluconate but also on oleic acid, suggesting that PhaG may play a role in this process, as well. Moreover, genes involved in the β-oxidation pathway were induced in the PHAs production phase when sodium gluconate was supplied as the only carbon and energy source. The transition from exponential growth to stationary phase caused a significant expression of genes involved in nitrogen metabolism, energy supply, and transport system. In this study, several molecular mechanisms, which drive mcl-PHAs synthesis, have been investigated. The identified genes may provide valuable information to improve the efficiency of this bioprocess and make it more economically feasible.Electronic supplementary materialThe online version of this article (10.1007/s10295-018-2042-4) contains supplementary material, which is available to authorized users.

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“…These central metabolites can be used as building blocks in recombinant E . coli for small molecules such as butanol (Shen et al ., ), or more complex chemical species such as polyhydroxyalkanoates [PHAs (Anjum et al ., ; Chen and Jiang, ), specially the simplest form of these polymers, poly(3‐hydroxybutyrate), PHB (Gomez et al ., )] and fatty acids (Sarria et al ., ). The strategies used for enhancing the formation of such metabolic precursors usually involve deleting reactions that deplete pyruvate or acetyl‐CoA or boosting carbon fluxes through glycolytic pathways (Sánchez‐Pascuala et al ., ).…”

Section: Bacterial Species Adopted As a Chassis: From Historical Exammentioning

confidence: 99%

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

Calero

Nikel

2018

Microbial Biotechnology

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The last few years have witnessed an unprecedented increase in the number of novel bacterial species that hold potential to be used for metabolic engineering. Historically, however, only a handful of bacteria have attained the acceptance and widespread use that are needed to fulfil the needs of industrial bioproduction - and only for the synthesis of very few, structurally simple compounds. One of the reasons for this unfortunate circumstance has been the dearth of tools for targeted genome engineering of bacterial chassis, and, nowadays, synthetic biology is significantly helping to bridge such knowledge gap. Against this background, in this review, we discuss the state of the art in the rational design and construction of robust bacterial chassis for metabolic engineering, presenting key examples of bacterial species that have secured a place in industrial bioproduction. The emergence of novel bacterial chassis is also considered at the light of the unique properties of their physiology and metabolism, and the practical applications in which they are expected to outperform other microbial platforms. Emerging opportunities, essential strategies to enable successful development of industrial phenotypes, and major challenges in the field of bacterial chassis development are also discussed, outlining the solutions that contemporary synthetic biology-guided metabolic engineering offers to tackle these issues.

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Microbial production of polyhydroxyalkanoates (PHAs) and its copolymers: A review of recent advancements

Cited by 537 publications

References 315 publications

X-ray Tomographic Imaging of Tensile Deformation Modes of Electrospun Biodegradable Polyester Fibers

X-ray Tomographic Imaging of Tensile Deformation Modes of Electrospun Biodegradable Polyester Fibers

Transcriptome remodeling of Pseudomonas putida KT2440 during mcl-PHAs synthesis: effect of different carbon sources and response to nitrogen stress

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

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