Microbial Methane Potential for the South Sumatra Basin Coal: Formation Water Screening and Coal Substrate Bioavailability

Susilawati, Rita; Esterle, Joan; Golding, Suzanne D.; Mares, Tennille E.

doi:10.1016/j.egypro.2015.01.051

Cited by 19 publications

(18 citation statements)

References 39 publications

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“…The enrichment cultures showed increasing CH 4 production over time with both hydrogenotrophic and acetoclastic pathways active (see Susilawati et al, 2015a;2015b). Overall CH 4 production from substrate tubes (coal, acetate, H 2 +CO 2 ) was higher than from the nocoal/no substrate control tubes (Tables 4, 5 and 6).…”

Section: Ch 4 Production and Methanogenic Substrate Screeningmentioning

confidence: 93%

“…Molecular DNA analysis showed that SSB formation waters contained diverse bacterial and archaeal communities that were able to generate CH 4 using different substrates including coal, acetate and H 2 +CO 2 (Figure 2) (Susilawati et al, 2015a (Susilawati et al, 2015a;2015b). At the genera level, Methanosarcina and Methanosaeta were the most prevalent archaeal methanogens in the SSB water, followed by Methanobacterium and Methanoregula.…”

Section: Microbial Methanogen Consortiamentioning

confidence: 99%

“…Culturing experiments were conducted using South Sumatra Basin (SSB) formation water as an inoculum and various carbon substrates (coal, acetate, H 2 +CO 2 ) ( Table 1). Water and coal samples used in the study were the same as those used in Susilawati et al (2015a;2015b), where details of water and coal sampling, as well as coalbed water chemistry, are described. Collection of coal directly from producing CBM reservoirs in the SSB was unfortunately not possible; therefore, formation water was used as an inoculum and coal from a nearby SSB coal mine was used as substrate.…”

Section: Experimental Overviewmentioning

confidence: 99%

“…This experiment was primarily designed as a screening for viable methanogen and coal to CH 4 activity in 5 SSB formation waters. The SSB2 cultures did not show any viable coal to CH 4 activity (see Susilawati et al, 2015a). Molecular DNA analysis data are available for experiment were inoculated using 8-month-old formation water from well SSB5 (Table 1).…”

Section: Experimental Overviewmentioning

confidence: 99%

“…Two papers have been published related to formation water screening and microbial community composition (Susilawati et al, 2015a;2015b). Here, the same materials are used to evaluate whether the isotopic compositions of CH 4 produced from coal-associated cultures exhibit biogenic signatures that are indicative for the defined methanogenesis pathway.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Carbon and hydrogen isotope fractionation during methanogenesis: A laboratory study using coal and formation water

Susilawati

Golding

Baublys

et al. 2016

International Journal of Coal Geology

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Carbon and hydrogen isotope compositions of CH 4 generated via methanogenesis in cultures of South Sumatra Basin (SSB) coalbed methane (CBM) formation waters grown on coal, acetate and H 2 +CO 2 were investigated. CH 4 production and molecular analysis confirmed the presence of active microbial communities that are able to convert coal into CH 4 using both acetoclastic and hydrogenotrophic pathways. The representative bacterial sequences were dominated by Bacteroidetes, Firmicutes and Deltaproteobacteria, while Methanosaeta and Methanosarcina were the most prevalent archaeal methanogens present in the cultures.CH 4 produced in this study's culturing experiments has δ 13 C values in the range of -50 ‰ to -20 ‰, with most values falling outside the current understanding of the carbon isotopic boundaries for biogenic CH 4 (-110 ‰ to -30 ‰). However, the corresponding apparent carbon isotopic α factor (α c = 1.02±0.006), and isotopic effect (ε c = -20.1 ‰ ±15.3) showed that CH 4 in SSB cultures was predominantly produced by acetoclastic methanogenesis, which is consistent with the results of molecular DNA analysis. In addition, the calculated contribution of CO 2 reduction from the δ 13 C values of coaltreated cultures was overall < 50 %, further confirming the high contribution of the acetoclastic pathway to CH 4 production in the SSB cultures. The outcome of this experimental study also suggests that δ 2 H-CH 4 values may not provide a reliable basis for distinguishing methanogenic pathways, while apparent carbon isotopic fractionation factor (α c ) and isotope effect (ε c ) are considered more useful indicators of the methanogenic pathway.The high δ 13 C-CH 4 values (>-30 ‰) and the dominance of Methanosaeta over Methanosarcina indicate that methanogens within the SSB cultures were operating at low substrate concentrations. An unusually positive δ 13 C-CH 4 suggests a substrate depletion effect, which is thought to be related to a decrease in the relative abundance of key bacterial coal degraders with formation water inoculum storage time. Closer observation of δ 13 C-CH 4 values during the growth of cultures within a single experiment also showed a 13 C-enrichment trend over time. At log phase of growth, the CH 4 produced was 13 C-depleted when compared to the stationary phase that also indicates substrate depletion effects. Finally, the δ 13 C-CH 4 values encountered in this study (as high as -20 ‰) highlight the possible positive extension of δ 13 C-CH 4 values of acetoclastic methanogenesis from those currently reported in the literature for natural and experimental samples (as high as -30 ‰).Keywords: Biogenic gas, coal, culture experiment, isotopic composition INTRODUCTIONArchaeal methanogens are the only known microorganisms capable of forming CH 4 . These strictly anaerobic microbes are restricted to utilizing simple compounds that contain no more than two carbon atoms. As such, complex organic compounds in anoxic environments (e.g., marine sediments, rice paddies, subsurface shales and coalbeds) ...

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Section: Ch 4 Production and Methanogenic Substrate Screeningmentioning

confidence: 93%

Section: Microbial Methanogen Consortiamentioning

confidence: 99%

Section: Experimental Overviewmentioning

confidence: 99%

Section: Experimental Overviewmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Carbon and hydrogen isotope fractionation during methanogenesis: A laboratory study using coal and formation water

Susilawati

Golding

Baublys

et al. 2016

International Journal of Coal Geology

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New insights into the coal-associated methane architect: the ancient archaebacteria

Mukherjee,

Selvi,

Ganguly

et al. 2024

Arch Microbiol

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Mechanisms of secondary biogenic coalbed methane formation in bituminous coal seams: a joint experimental and multi-omics study

Zhang,

Liu,

Xue

et al. 2024

Arch Microbiol

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Coal seam microbes, as endogenous drivers of secondary biogenic gas production in coal seams, might be related to methane production in coal seams. In this study, we carried out anaerobic indoor culture experiments of microorganisms from three different depths of bituminous coal seams in Huainan mining area, and revealed the secondary biogas generation mechanism of bituminous coal seams by using the combined analysis of macro-genome and metabolism multi-omics. The results showed that the cumulative mass molar concentrations (Molality) of biomethane production increased with the increase of the coal seam depth in two consecutive cycles; At the genus level, there were significant differences in the bacterial and archaeal community structures corresponding to the three coal seams 1#, 6#, and 9#, with correlations of R bacterial = 0.368 and R archaeal = 0.463, respectively; V ad and V i of coal were significantly correlated with differences in genus-level composition of bacteria and archaea; the largest difference in functional genes related to the methanogenic metabolic pathway was observed before and after incubation of coal bed microorganisms, with an average positive growth of 42%; meanwhile, a total of 11 classes of carbon metabolism homologues closely related to methanogenesis were detected in the liquid metabolites of coal bed microbes after 60 days of incubation. Finally, the fatty acid, amino acid and carbohydrate synergistic methanogenic metabolic pathway was reconstructed based on KEGG database; the expression level of mcrA gene within the metabolic pathway of the 1# deep coal sample was significantly higher than that of the other two groups (p<0.05 for significance), and the efficient expression of this functional gene at the front end of the methanogenic pathway facilitates the conversion of bituminous coal organic matter to methane.Therefore, coal quality (V ad ) is likely to be one of the key factors causing diversity in microbial community composition and metabolic function differences in different coal seams.

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Microbial Methane Potential for the South Sumatra Basin Coal: Formation Water Screening and Coal Substrate Bioavailability

Cited by 19 publications

References 39 publications

Carbon and hydrogen isotope fractionation during methanogenesis: A laboratory study using coal and formation water

Carbon and hydrogen isotope fractionation during methanogenesis: A laboratory study using coal and formation water

New insights into the coal-associated methane architect: the ancient archaebacteria

Mechanisms of secondary biogenic coalbed methane formation in bituminous coal seams: a joint experimental and multi-omics study

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