Microbial Forensics: Application to Bioterrorism Preparedness and Response

Morse, Stephen; Budowle, Bruce

doi:10.1016/j.idc.2006.03.004

Cited by 31 publications

(10 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Resequencing of these regions revealed that the EDL933 and Sakai genome sequences were identical at each of the 17 positions, demonstrating directly that these particular sites, rather than SNPs, were sequencing errors in either the EDL933 or the Sakai sequence. 1 These data serve to point out not only the need for independent verification of sequence data in the open databases but also the utility of tiling arrays for identifying potential sequencing errors.…”

Section: Tiling Arrays Of Sequenced Genomesmentioning

confidence: 99%

“…Reliable and valid methods are therefore needed for the tracking and identification of individual strains of foodborne pathogens, a requirement for the forensic investigation of such an attack [1][2][3]. Properties of Escherichia coli O157:H7, i.e., its low infectious dose, ease of transmissibility, and its known incidence in non-intentional outbreaks of human disease, make this pathogen a potential formidable biothreat agent.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Interrogating genomic diversity of E. coli O157:H7 using DNA tiling arrays

Jackson

Mammel

Patel

et al. 2007

Forensic Science International

View full text Add to dashboard Cite

Here, we describe a novel microarray-based approach for investigating the genomic diversity of Escherichia coli O157:H7 in a semi-high throughput manner using a high density, oligonucleotide-based microarray. This microarray, designed to detect polymorphisms at each of 60,000 base-pair (bp) positions within an E. coli genome, is composed of overlapping 29-mer oligonucleotides specific for 60 equally spaced, 1000-bp loci of the E. coli O157:H7 strain EDL933 chromosome. By use of a novel 12-well microarray that permitted the simultaneous investigation of 12 strains, the genomes of 44 individual isolates of E. coli O157:H7 were interrogated. These analyses revealed more than 150 single nucleotide polymorphisms (SNPs) and several deletions and amplifications in the test strains. Pyrosequencing was used to confirm the usefulness of the novel SNPs by determining their allelic frequency among a collection of diverse isolates of E. coli O157:H7. The tiling DNA microarray system would be useful for the tracking and identification of individual strains of E. coli O157:H7 needed for forensic investigations. #

show abstract

Section: Tiling Arrays Of Sequenced Genomesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Interrogating genomic diversity of E. coli O157:H7 using DNA tiling arrays

Jackson

Mammel

Patel

et al. 2007

Forensic Science International

View full text Add to dashboard Cite

show abstract

“…The anthrax-letters attack of 2001 demonstrated the public's vulnerability to such attacks and the U.S. government's inability to forensically investigate the evidence for attribution purposes (66). This resulted in the birth of the field of microbial forensics.…”

Section: Microbial Forensics and High-throughput Sequencingmentioning

confidence: 99%

Extracting evidence from forensic DNA analyses: future molecular biology directions

Budowle

Daal

2009

BioTechniques

Self Cite

View full text Add to dashboard Cite

Molecular biology tools have enhanced the capability of the forensic scientist to characterize biological evidence to the point where it is feasible to analyze minute samples and achieve high levels of individualization. Even with the forensic DNA field's maturity, there still are a number of areas where improvements can be made. These include: enabling the typing of samples of limited quantity and quality; using genetic information and novel markers to provide investigative leads; enhancing automation with robotics, different chemistries, and better software tools; employing alternate platforms for typing DNA samples; developing integrated microfluidic/microfabrication devices to process DNA samples with higher throughput, faster turnaround times, lower risk of contamination, reduced labor, and less consumption of evidentiary samples; and exploiting high-throughput sequencing, particularly for attribution in microbial forensics cases. Knowledge gaps and new directions have been identified where molecular biology will likely guide the field of forensics. This review aims to provide a roadmap to guide those interested in contributing to the further development of forensic genetics.

show abstract

“…Si realizzò, così, un'infrastruttura caratterizzata da tools analitici e conoscenze che ebbero come scopo l'esecuzione di un'indagine sugli eventi più rapida ed 6,7 . Denominatore comune di questo network di competenze fu una nuova disciplina, definita microbial forensics, che si poneva l'obiettivo di fornire gli elementi necessari all'indagine giudiziaria per attribuire a uno specifico colpevole [7][8][9] il crimine perpetrato attraverso l'utilizzo di un agente biologico. In realtà l'FBI aveva già sfruttato questo tipo di approccio in seguito all'emergenza dei reati sessuali sorta a metà degli anni Novanta, istituendo un gruppo di lavoro specializzato.…”

Section: La Nuova Frontiera Dei Microbial Forensics: Un Network DI Counclassified