Microbial Control of Black Flies (Diptera: Simuliidae) With Bacillus thuringiensis subsp. israelensis

Gray, Elmer W.; Fusco, Robert A.

doi:10.1016/b978-0-12-803527-6.00025-1

Cited by 4 publications

(6 citation statements)

References 43 publications

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“…Наші дані не суперечать результатам інших дослідників про те, що найефективнішою та екологічно прийнятною технікою пригнічення популяцій мошок є застосування ларвіцидів Bacillus thuringiensis subsp. israelensis (Bti) у річках і струмках, де розвиваються паразити [4]. Застосування ларвіцидів здійснюється в рамках інтегрованої боротьби з кровососами, а результати спостереження за личинками та імаго використовуються для максимально ефективного впливу на популяцію ектопаразитів.…”

Section: обговоренняunclassified

Bacterial infection of black flies larvae and bacterial preparations application for quantity control of Simuliidae

Сухомлін,

Зінченко,

Теплюк

et al. 2024

NCBio

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Considerable attention is paid to the biological method of controlling the number of blood-sucking insects, as it is highly selective, effective and environmentally safe. 340 reservoirs were examined within Volyn Polissia, among which 86 (14,9%) contained Simuliidae larvae with signs of bacterial lesion. Damaged larvae were recorded in small rivers and ameliorative canals, most of which, 71,5%, were mesosaprobic in terms of water quality. Bacterial infection was detected in the larvae of 10 species: S. pusillum, S. nigrum, S. equinum, S. erythrocephalum, S. ornatum, S. dolini, S. noelleri S. paramorsitans, S. promorsitans, S. morsitans. Оn Volyn Polissia a study was conducted on the effectiveness of reservoirs delarvation with three preparations based on Bacillus thuringiensis H14 bacteria - bactoculicide, bactolarvicide and BLP-2477. Experiments were carried out in ameliorative canals with a bed up to 2 m wide, 0.5 m deep and with a current speed of 0.7 m/s, which were densely populated by preimaginal phases of black flies. Treatment of the reservoir with preparations was carried out in two ways. The first consists in a one-time introduction of the entire dose, the second - in a gradual introduction of the dose over 6 and 24 hours. It was established that a single application of the entire dose of the preparation ensures only partial death (up to 75%) of black flies. An uneven death of larvae was registered in the entire area of treatment. The method of gradual introduction of the preparation, when it is washed out over 6 or 24 hours, is more effective (death of larvae up to 100%). To the quantity control of the black flies pre-imaginal phases development, preparations BLP-2477 and bactolarvicide are recommended as the most effective. The optimal dose for the death of black flies larvae at a distance of up to 1.5 km in ameliorative canals with width of up to 2 m, depth of 0.5 m and a flow speed of 0.7 m/s should be considered 400 g of the preparation. The deaths of non-target hydrobionts, caddisflies, mayflies, molluscs, and fishes were not registered. Key words: Simuliidae, Bacillus thuringiensis, reservoirs delarvation, bactoculicide, bactolarvicide, BLP-2477.

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Section: обговоренняunclassified

Bacterial infection of black flies larvae and bacterial preparations application for quantity control of Simuliidae

Сухомлін,

Зінченко,

Теплюк

et al. 2024

NCBio

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“…Bacillus thuringiensis serovar israelensis (Bti) is one of the subspecies' most effective larvicides for mosquito control, being recommended by the World Health Organization (WHO) [4,5]. Bti produces Cry and Cyt crystals (Cry4Aa, Cry4Ba, Cry10Aa, Cry11Aa, Cyt1Aa, and Cyt2Ba) that exhibit toxicity against mosquito species from the genus Aedes, Anopheles, and Culex, and for the black fly (Simuliidae) [4,[6][7][8]. These proteins synergistically interact and can decrease the incidence of resistance in insect populations [4].…”

Section: Introductionmentioning

confidence: 99%

Isolation, Genomic, and Proteomic Characterization of a Novel Neotropical Strain of Bacillus thuringiensis with Mosquitocidal Activities

et al. 2023

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The combination of genomic and proteomic analyses is a useful tool for the study of novel Bacillus thuringiensis (Bt) strains, as these approaches allow the accurate identification of pesticidal proteins and virulence factors produced. Here, we isolated and evaluated the potential of a novel Neotropical Bt strain (TOD651) for controlling larvae of Aedes aegypti and Culex quinquefasciatus mosquitoes. Aiming for the full comprehension of the TOD651 larvicidal potential, we further evaluated the whole TOD651 genome and conducted the proteomic analysis of the TOD651 spore–crystal mixtures. Our results showed that Bt TOD651 similarly killed both A. aegypti (0.011 µg/mL) and C. quinquefasciatus (0.023 µg/mL) larvae, exhibiting similar potency to the commercial Bt strain. The genome sequence revealed that Bt TOD651 harbors cry11Aa3, cry10Aa4, cry4Aa4, cry4Ba5, cyt1Aa5, cyt1Ca1, cyt2Ba13, mpp60Aa3, and mpp60Ba3. The proteomic analysis revealed no expression of Mpp60Aa3, while all the other pesticidal proteins were expressed (Cry4Ba5 was more abundant than Cyt1Aa5). The expression of the Mppe showed the major proportions between proteases. The virulent factor neutral protease B and spore coat proteins were also expressed. The expression of relevant pesticidal proteins (e.g., Cry, Cyt, Mpp, and other pathogenic factors), whose actions can occur in a synergic relation, indicates that the biocontrol using Bt TOD651 may contribute to delaying the selection of resistant individuals.

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“…pest management (IPM), he was one of the first to investigate Bacillus thuringiensis (Bt) as a lesstoxic alternative to chemical mosquito and black fly control 7,10 -a method that is now widespread and continues to be recommended (Gray & Fusco 2017;Lawler 2017).…”

mentioning

confidence: 99%

Professor Emeritus James W. Amrine Jr.: advancing the study of Varroa destructor and eriophyoid mites for over 30 years

Sarratt

Zhao²,

Ochoa³

2022

saa

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As the world’s foremost taxonomist of eriophyoid mites (Eriophyoidea, Acariformes) (Xue et al. 2017), Dr. James (Jim) Wesley Amrine Jr.’s name is nearly synonymous with the group. His experiments with Phyllocoptes fructiphilus (Eriophyidae) as well as Varroa destructor (Varroidae, Mesostigmata, Parasitiformes) (Zhang et al. 2011) demonstrate Jim’s commitment to developing practical applications of Acari research. In recognition of these and other acarological impacts, we nominate Dr. Amrine for the James A. McMurtry Award. Here we review highlights of his remarkable career, and celebrate his scientific as well as personal contributions.

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Microbial Control of Black Flies (Diptera: Simuliidae) With Bacillus thuringiensis subsp. israelensis

Cited by 4 publications

References 43 publications

Bacterial infection of black flies larvae and bacterial preparations application for quantity control of Simuliidae

Bacterial infection of black flies larvae and bacterial preparations application for quantity control of Simuliidae

Isolation, Genomic, and Proteomic Characterization of a Novel Neotropical Strain of Bacillus thuringiensis with Mosquitocidal Activities

Professor Emeritus James W. Amrine Jr.: advancing the study of Varroa destructor and eriophyoid mites for over 30 years

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