Microbial Contamination of Bivalve Mollusks Used for Human Consumption

Roldán, Elena Moreno; Rodríguez, Elena Espigares; Vicente, Carmen Navarro; Navajas, Milagros Fernández-Crehuet; Abril, Obdulia Moreno

doi:10.1111/j.1745-4565.2010.00294.x

Cited by 13 publications

(8 citation statements)

References 6 publications

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“…Bivalve molluscs are especially prone to act as transmitters of human disease-causing pathogens [29] . The waters they inhabit are often exposed to contamination by faecal matter from sewer drains or from infected individuals.…”

Section: Resultsmentioning

confidence: 99%

“…The waters they inhabit are often exposed to contamination by faecal matter from sewer drains or from infected individuals. It has been widely shown that not only the consumption of contaminated seafood can cause diseases in humans, but this can likely represent a possible cause of molluscs high mortality events [29] . The pathogenic species more often reported in C. gallina are Marteilia refringens (Rhizaria, Canadian Food Inspection Agency, http://www.inspection.gc.ca ), Cryptosporidium spp.…”

Section: Resultsmentioning

confidence: 99%

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Sequencing and Characterization of Striped Venus Transcriptome Expand Resources for Clam Fishery Genetics

Coppe¹,

Bortoluzzi²,

Murari³

et al. 2012

PLoS ONE

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BackgroundThe striped venus Chamelea gallina clam fishery is among the oldest and the largest in the Mediterranean Sea, particularly in the inshore waters of northern Adriatic Sea. The high fishing pressure has lead to a strong stock abundance decline, enhanced by several irregular mortality events. The nearly complete lack of molecular characterization limits the available genetic resources for C. gallina. We achieved the first transcriptome of this species with the aim of identifying an informative set of expressed genes, potential markers to assess genetic structure of natural populations and molecular resources for pathogenic contamination detection.Methodology/Principal FindingsThe 454-pyrosequencing of a normalized cDNA library of a pool C. gallina adult individuals yielded 298,494 raw reads. Different steps of reads assembly and filtering produced 36,422 contigs of high quality, one half of which (18,196) were annotated by similarity. A total of 111 microsatellites and 20,377 putative SNPs were identified. A panel of 13 polymorphic transcript-linked microsatellites was developed and their variability assessed in 12 individuals. Remarkably, a scan to search for contamination sequences of infectious origin indicated the presence of several Vibrionales species reported to be among the most frequent clam pathogen's species. Results reported in this study were included in a dedicated database available at http://compgen.bio.unipd.it/chameleabase.Conclusions/SignificanceThis study represents the first attempt to sequence and de novo annotate the transcriptome of the clam C. gallina. The availability of this transcriptome opens new perspectives in the study of biochemical and physiological role of gene products and their responses to large and small-scale environmental stress in C. gallina, with high throughput experiments such as custom microarray or targeted re-sequencing. Molecular markers, such as the already optimized EST-linked microsatellites and the discovered SNPs will be useful to estimate effects of demographic processes and to detect minute levels of population structuring.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Sequencing and Characterization of Striped Venus Transcriptome Expand Resources for Clam Fishery Genetics

Coppe¹,

Bortoluzzi²,

Murari³

et al. 2012

PLoS ONE

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“…Therefore, KA and its derivatives have attracted much attention for their variety of biological activities.In aquatic product preservation, the most common challenges are water retention and bacterial inhibition. Foodborne pathogens and spoilage bacteria such as Staphylococcus aureus, Shewanella putrefaciens, Escherichia coli, and Salmonella enterica, which are common strains found in aquatic products [20][21][22]. Staphylococcus epidermidis is a bacterium that breeds on the epidermis of organisms.…”

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confidence: 99%

Synthesis, Antimicrobial, Moisture Absorption and Retention Activities of Kojic Acid-Grafted Konjac Glucomannan Oligosaccharides

et al. 2019

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Kojic acid (KA) with antibacterial activities produced by fermentation was grafted onto konjac glucomannan oligosaccharide (KGO) composed of glucose and mannose linked by β-1,4 glycosidic bonds. A novel KGO derivative, konjac glucomannan oligosaccharide kojic acid (KGOK) possessing both moisture retention and antibacterial activities was synthesized. The structure of KGOK was characterized and analyzed by thermogravimetric analysis (TG), XRD, UV-vis absorption, FTIR, and 1 H NMR. The analysis results suggest that KA was linked to the KGO molecular chain through a covalent bond, and the reaction site of KA was the methylol group. The studies demonstrate that KGOK maintained the excellent moisture absorption and retention properties of KGO and the good antibacterial activities of KA. The minimum inhibitory concentration (MIC) of KGOK is 2 mg/mL for Staphylococcus aureus, Staphylococcus epidermidis, Shewanella putrefaciens, and Salmonella enterica, while its MIC is 3 mg/mL for Escherichia coli. The multi-functionality of the KGOK synthesized from natural sources provides a theoretical foundation for their potential applications in the preservation of food, beverage, aquatic, and cosmetic products.Polymers 2019, 11, 1979 2 of 12 and KGO have many good properties, these functionalities can be further improved through certain molecular modifications. Various forms of KGM derivatives obtained by physical, chemical, and enzymatical modifications have been used in a range of different applications, including biodegradable films, medical and pharmaceutical materials, functional foods, and aerogel [10]. Thus, the modification of KGM and KGO gains much interest from numerous researchers because of their better performance in food applications.Kojic acid (KA), 5-hydroxy-2-hydroxymethyl-1,4-pyrone, is a weakly acidic secondary metabolite produced by aerobic fermentation of Aspergillus, Acetobacter, and Penicillium. KA has a variety of biological activities, including antibacterial, antifungal, antiviral, and antioxidant activities [16][17][18][19]. Therefore, KA and its derivatives have attracted much attention for their variety of biological activities.In aquatic product preservation, the most common challenges are water retention and bacterial inhibition. Foodborne pathogens and spoilage bacteria such as Staphylococcus aureus, Shewanella putrefaciens, Escherichia coli, and Salmonella enterica, which are common strains found in aquatic products [20][21][22]. Staphylococcus epidermidis is a bacterium that breeds on the epidermis of organisms. Most of them are non-pathogenic bacteria and a few can cause diseases. S. aureus is a commensal and opportunistic pathogen that can cause contamination of food products during preparation and processing [23]. S. putrefaciens is a marine, gram-negative bacterium which plays a role in the food industry as a spoilage bacterium of marine fish and chicken due to its ability to produce volatile sulfides, amines, and the fishy-smelling compound trimethylamine [24]. As one of the oligosaccharides...

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“…Twenty-five grams of the mollusc body (stomach and digestive diverticulum) were homogenized with 200 mL of 0.1% peptone water (pH 7.0 -0.2) in a Stomacher blender (Stomacher 400 Circulator, Seward Limited, Norfolk, UK) for 2 min at 150 rpm (Moreno Roldán et al, 2011;CEN, 2003). The supernatant was divided into 8-mL aliquots, and 2 mL of glycerol was added to each aliquot.…”

Section: Methodsmentioning

confidence: 99%

Prevalence of Hepatitis A Virus in Bivalve Molluscs Sold in Granada (Spain) Fish Markets

Roldán

Rodríguez²,

García³

et al. 2013

Foodborne Pathogens and Disease

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Viruses are the leading cause of foodborne illness associated with the consumption of raw or slightly cooked contaminated shellfish. The aim of this study was to evaluate the prevalence of hepatitis A virus in molluscs. Standard and real-time reverse transcription-polymerase chain reaction procedures were used to monitor bivalve molluscs from the Granada fish markets (southern Spain) for this human enteric virus. Between February 2009 and October 2010, we collected a total of 329 samples of different types of bivalve molluscs (mussels, smooth clams, striped venus, and grooved clams). The results showed the presence of hepatitis A virus in 8.5% of the 329 samples analyzed. We can therefore confirm that conventional fecal indicators are unreliable for demonstrating the presence or absence of viruses. The presence of hepatitis A virus in molluscs destined for human consumption is a potential health risk in southern Spain.

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Microbial Contamination of Bivalve Mollusks Used for Human Consumption

Cited by 13 publications

References 6 publications

Sequencing and Characterization of Striped Venus Transcriptome Expand Resources for Clam Fishery Genetics

Sequencing and Characterization of Striped Venus Transcriptome Expand Resources for Clam Fishery Genetics

Synthesis, Antimicrobial, Moisture Absorption and Retention Activities of Kojic Acid-Grafted Konjac Glucomannan Oligosaccharides

Prevalence of Hepatitis A Virus in Bivalve Molluscs Sold in Granada (Spain) Fish Markets

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