Microbial Community Composition, Functions, and Activities in the Gulf of Mexico 1 Year after the Deepwater Horizon Accident

Yergeau, Étienne; Maynard, Christine; Sanschagrin, Sylvie; Champagne, Julie; Juck, David; Lee, Kenneth; Greer, Charles W.

doi:10.1128/aem.01470-15

Cited by 57 publications

(58 citation statements)

References 41 publications

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“…Interestingly, Cycloclasticus spp. were often reported as one of the predominant PAH degraders in seawater (37, 38), whereas Mycobacterium are typical soil PAH degraders (39) and Massilia were found to degrade PAHs in soils or associated to roots (40, 41). This diversity of putative PAH degraders associated with the rhizosphere of Spartina is probably linked to the nature of its habitat, at the interface of terrestrial, plant and maritime ecosystems.…”

Section: Discussionmentioning

confidence: 99%

Phenanthrene contamination and ploidy level influence the rhizosphere microbiome of Spartina

Cavé‐Radet¹,

Monard²,

Amrani³

et al. 2019

Preprint

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14Spartina spp. are widely distributed salt marsh plants that have a recent history of 15 hybridization and polyploidization. These evolutionary events have resulted in species with a 16 heightened resilience to hydrocarbon contamination, which could make them an ideal model 17 plant for the phytoremediation/reclamation of contaminated coastal ecosystems. However, it 18 is still unknown if allopolyploidization events also resulted in differences in the plant 19 rhizosphere-associated microbial communities, and if this could improve the plant 20 phytoremediation potential. Here, we grew two parental Spartina species, their hybrid and the 21 resulting allopolyploid in salt marsh sediments that were contaminated or not with 22 phenanthrene, a model tricyclic PAH. The DNA from the rhizosphere soil was extracted and 23 the bacterial 16S rRNA gene and ITS region were amplified and sequenced. Generally, both 24 the presence of phenanthrene and the identity of the plant species had significant influences 25 on the bacterial and fungal community structure, composition and diversity. In particular, the 26 allopolyploid S. anglica, harbored a more diverse bacterial community in its rhizosphere, and 27 relatively higher abundance of various bacterial and fungal taxa. Putative hydrocarbon 28 degraders were significantly more abundant in the rhizosphere soil contaminated with 29 phenanthrene, with the Nocardia genus being significantly more abundant in the rhizosphere 30 of S. anglica. Overall our results are showing that the recent polyploidization events in the 31 Spartina did influence the rhizosphere microbiome, both under normal and contaminated 32 conditions, but more work will be necessary to confirm if these differences result in a higher 33 phytoremediation potential. 34 35 36 3 Importance 37Salt marshes are at the forefront of coastal contamination events caused by marine oil spills. 38Microbes in these environments play a key role in the natural attenuation of these 39 contamination events, often in association with plant roots. One such plant is the Spartina, 40 which are widely distributed salt marsh plants. Intriguingly, some species of the Spartina 41 show heightened resistance to contamination, which we hypothesized to be due to differences 42 in their microbiota. This was indeed the case, with the most resistant Spartina also showing 43 the most different microbiota. A better understanding of the relationships between the 44 Spartina and their microbiota could improve the coastal oil spill clean-up strategies and 45 provide green alternatives to more traditional physico-chemical approaches.46 47 48 49 Polycyclic aromatic hydrocarbons (PAHs) are a group of ubiquitous organic pollutants, that 50 are highly concerning in view of their potentially severe impact on natural ecosystems and 51 public health. The remediation of sites contaminated by these compounds due to human 52 activity, such as oil spill, is mainly carried out through excavation, soil leaching or various 53 techniques based on microbial degra...

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Section: Discussionmentioning

confidence: 99%

Phenanthrene contamination and ploidy level influence the rhizosphere microbiome of Spartina

Cavé‐Radet¹,

Monard²,

Amrani³

et al. 2019

Preprint

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“…2013) and more recently, Yergeau et al. (2015) reported this community to be dominant in the polluted area they studied.…”

Section: Microbial Ecology Of Pah-polluted Marine Ecosystemsmentioning

confidence: 91%

Role of environmental factors and microorganisms in determining the fate of polycyclic aromatic hydrocarbons in the marine environment

Duran¹,

Cravo‐Laureau²

2016

FEMS Microbiology Reviews

205

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Polycyclic aromatic hydrocarbons (PAHs) are widespread in marine ecosystems and originate from natural sources and anthropogenic activities. PAHs enter the marine environment in two main ways, corresponding to chronic pollution or acute pollution by oil spills. The global PAH fluxes in marine environments are controlled by the microbial degradation and the biological pump, which plays a role in particle settling and in sequestration through bioaccumulation. Due to their low water solubility and hydrophobic nature, PAHs tightly adhere to sediments leading to accumulation in coastal and deep sediments. Microbial assemblages play an important role in determining the fate of PAHs in water and sediments, supporting the functioning of biogeochemical cycles and the microbial loop. This review summarises the knowledge recently acquired in terms of both chronic and acute PAH pollution. The importance of the microbial ecology in PAH-polluted marine ecosystems is highlighted as well as the importance of gaining further in-depth knowledge of the environmental services provided by microorganisms.

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“…Only a few studies regarding bacteria in marine oil‐contaminated environments under high‐pressure conditions have been performed (Grossi et al., ; Schedler, Hiessl, Valladares Juárez, Gust, & Müller, ). Oil biodegradation has been extensively investigated at atmospheric pressure of 0.1 MPa, including studies performed using DWH samples (Atlas & Razen, ; Gutierrez et al., ; Joye, Teske, & Kostka, ; Redmond & Valentine, ; Scoma et al., ; Tapilatu et al., ; Yergeau et al., ; and many others), probably because of the lack of specialized high‐pressure lab technology. Using high‐pressure reactors, a progressive decrease in the degradation rate of hexadecane was observed in two strains ( Rhodococcus qingshengii and Sphingobium yanoikuyae ) as pressure increased (Schedler et al., ).…”

Section: Introductionmentioning

confidence: 99%

Response of marine bacteria to oil contamination and to high pressure and low temperature deep sea conditions

Fasca

Castilho

et al. 2017

MicrobiologyOpen

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The effect of pressure and temperature on microbial communities of marine environments contaminated with petroleum hydrocarbons is understudied. This study aims to reveal the responses of marine bacterial communities to low temperature, high pressure, and contamination with petroleum hydrocarbons using seawater samples collected near an offshore Brazilian platform. Microcosms containing only seawater and those containing seawater contaminated with 1% crude oil were subjected to three different treatments of temperature and pressure as follows: (1) 22°C/0.1 MPa; (2) 4°C/0.1 MPa; and (3) 4°C/22 MPa. The effect of depressurization followed by repressurization on bacterial communities was also evaluated (4°C/22 MPaD). The structure and composition of the bacterial communities in the different microcosms were analyzed by PCR‐DGGE and DNA sequencing, respectively. Contamination with oil influenced the structure of the bacterial communities in microcosms incubated either at 4°C or 22°C and at low pressure. Incubation at low temperature and high pressure greatly influenced the structure of bacterial communities even in the absence of oil contamination. The 4°C/22 MPa and 4°C/22 MPaD treatments resulted in similar DGGE profiles. DNA sequencing (after 40 days of incubation) revealed that the diversity and relative abundance of bacterial genera were related to the presence or absence of oil contamination in the nonpressurized treatments. In contrast, the variation in the relative abundances of bacterial genera in the 4°C/22 MPa‐microcosms either contaminated or not with crude oil was less evident. The highest relative abundance of the phylum Bacteroidetes was observed in the 4°C/22 MPa treatment.

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Microbial Community Composition, Functions, and Activities in the Gulf of Mexico 1 Year after the Deepwater Horizon Accident

Cited by 57 publications

References 41 publications

Phenanthrene contamination and ploidy level influence the rhizosphere microbiome of Spartina

Phenanthrene contamination and ploidy level influence the rhizosphere microbiome of Spartina

Role of environmental factors and microorganisms in determining the fate of polycyclic aromatic hydrocarbons in the marine environment

Response of marine bacteria to oil contamination and to high pressure and low temperature deep sea conditions

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