Microbial community analysis in the denitrification process of saline-wastewater by denaturing gradient gel electrophoresis of PCR-amplified 16S rDNA and the cultivation method

Yoshie, Sachiko; Noda, Naohiro; Miyano, Tomoko; Tsuneda, Satoshi; Hirata, Akira; Inamori, Yuhei

doi:10.1016/s1389-1723(01)80238-3

Cited by 38 publications

(22 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T-RFLP) profiles, and clone analysis based on 16S rRNA-encoding genes have been studied for the sludge of the denitrification system with 10% salinity (Yoshie et al, 2001). They investigated that the g-proteobacteria, particularly Halomonas spp., were predominant species for the high-rate denitrification of saline industrial wastewater and the 4-10% salinity resulted in the highest denitrification rate.…”

Section: Nutrient Removalmentioning

confidence: 99%

Progress in decontamination by halophilic microorganisms in saline wastewater and soil

Zhuang

Han

Zhang

et al. 2010

Environmental Pollution

145

View full text Add to dashboard Cite

Review on the decontaminative capabilities of halophilic microorganisms in saline wastewater and soil. a r t i c l e i n f o a b s t r a c tEnvironments with high-salt concentrations are often populated by dense microbial communities. Halophilic microorganisms can be isolated from different saline environments and different strains even belonging to the same genus have various applications. Wastewater and soil rich in both organic matter and salt are difficult to treat using conventional microorganisms typically found in wastewater treatment and soil bioremediation facilities. Studies on decontaminative capabilities and decontamination pathways of organic contaminants (i.e., aromatic compounds benzoate, cinnamate, 3-phenylpropionate, 4-hydroxybenzoic acid), heavy metals (i.e., tellurium, vanadium), and nutrients in the biological treatment of saline wastewater and soil by halophilic microorganisms are discussed in this review.

show abstract

Section: Nutrient Removalmentioning

confidence: 99%

Progress in decontamination by halophilic microorganisms in saline wastewater and soil

Zhuang

Han

Zhang

et al. 2010

Environmental Pollution

145

View full text Add to dashboard Cite

show abstract

“…Although we have developed a nitrogen removal system for saline wastewater, nitrite often accumulates, particularly under relatively high-saline conditions (32). Therefore, it is important to understand the ecology of nitrite-reducing bacterial communities to determine stable operational conditions for denitrification processes.A few researchers and we have studied microbial communities in anaerobic reactors for the denitrification of saline wastewater (13,31,32) and used an approach based on taxonomic analysis of the 16S rRNA gene to identify all of the bacterial species within a community. These studies suggested that members of the gamma subdivision of the class Proteobacteria are important in such wastewater treatment systems.…”

mentioning

confidence: 99%

“…A few researchers and we have studied microbial communities in anaerobic reactors for the denitrification of saline wastewater (13,31,32) and used an approach based on taxonomic analysis of the 16S rRNA gene to identify all of the bacterial species within a community. These studies suggested that members of the gamma subdivision of the class Proteobacteria are important in such wastewater treatment systems.…”

mentioning

confidence: 99%

Salinity Decreases Nitrite Reductase Gene Diversity in Denitrifying Bacteria of Wastewater Treatment Systems

Yoshie

Noda

Tsuneda

et al. 2004

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

Investigation of the diversity of nirK and nirS in denitrifying bacteria revealed that salinity decreased the diversity in a nitrate-containing saline wastewater treatment system. The predominant nirS clone was related to nirS derived from marine bacteria, and the predominant nirK clone was related to nirK of the genus Alcaligenes.Nitrogen removal from wastewater is accomplished by bacterial denitrification. Some types of industrial wastewater, such as metallurgic wastewater, contain large amounts of nitrate and saline (11). Although we have developed a nitrogen removal system for saline wastewater, nitrite often accumulates, particularly under relatively high-saline conditions (32). Therefore, it is important to understand the ecology of nitrite-reducing bacterial communities to determine stable operational conditions for denitrification processes.A few researchers and we have studied microbial communities in anaerobic reactors for the denitrification of saline wastewater (13,31,32) and used an approach based on taxonomic analysis of the 16S rRNA gene to identify all of the bacterial species within a community. These studies suggested that members of the gamma subdivision of the class Proteobacteria are important in such wastewater treatment systems. However, 16S rRNA gene-based approaches are unable to detect denitrifying bacteria particularly in such systems because bacterial groups possessing denitrifying abilities are phylogenetically diverse (33). Furthermore, although aerobic halophilic species have been reported (7,29), the microbial ecology of moderately halophilic denitrifying bacteria has hardly been reported.Recently, primer sets specific for functional genes involved in denitrification, namely, nirK, nirS (3, 9), and nosZ (22), have been developed. Thus, PCR-based approaches revealed denitrifying microbial communities in activated sludge (9, 27), marine sediment (4, 5, 15), and soil (17, 19) on the basis of nirK and nirS and in marine sediment on the basis of nosZ (23, 24). These approaches have contributed to the improvement of the complete or partial sequences of nitrite reductase genes and nitrous oxide reductase genes in the database. We expect that the information on these genes detected from various environments will contribute to further studies on the ecophysiology of denitrifying communities.In this study, we focused on the microbial ecology of nitritereducing bacteria in two series of metallurgic wastewater treatment systems (MWTSs) with different fluidity conditions; one of the MWTSs was composed of an anaerobic packed bed, and the other was composed of an anaerobic fluidized bed (31). The nirK and nirS heterogeneity in the anaerobic reactors of MWTSs was investigated by cloning, sequencing, and phylogenetic analysis to determine the actual denitrifying bacterial community. Furthermore, the anaerobic packed bed and the fluidized bed were compared for nirK and nirS diversity to investigate the influence of fluidity conditions on the denitrifying microbial community.Sludge samples and isola...

show abstract

“…We used a DNA microarray to analyze activated sludge samples of saline industrial wastewater, which had been studied previously [22][23][24][25] . Samples were taken at 21 and 130 days from the start of the reactor's operation 22) .…”

Section: Hybridization With Environmental Samplesmentioning

confidence: 99%

“…Then, hybridization and washing conditions were optimized using cultured reference microorganisms. Furthermore, samples of saline industrial wastewater, which were previously characterized using molecular-based approaches [22][23][24][25] , were examined with the small-scale oligonucleotide microarray.…”

mentioning

confidence: 99%

Direct Profiling of rRNA in Saline Wastewater Treatment Samples Using an Oligonucleotide Microarray

et al. 2007

Self Cite

View full text Add to dashboard Cite

Fifteen oligonucleotide probes, targeting a broad range of microorganisms, were spotted on a DNA microarray. Specificity and reproducibility were evaluated using fluorescently labeled rRNA from 16 bacterial and eukaryal strains. The oligonucleotide microarray was then used to analyze activated sludge samples of saline industrial wastewater in which microbial communities had been characterized previously using 16S rRNA clone libraries. The results obtained were partially consistent with the results of cloning, and demonstrate the possibility of using oligonucleotide microarrays for the monitoring and characterization of saline industrial wastewater populations.

show abstract

Microbial community analysis in the denitrification process of saline-wastewater by denaturing gradient gel electrophoresis of PCR-amplified 16S rDNA and the cultivation method

Cited by 38 publications

References 21 publications

Progress in decontamination by halophilic microorganisms in saline wastewater and soil

Progress in decontamination by halophilic microorganisms in saline wastewater and soil

Salinity Decreases Nitrite Reductase Gene Diversity in Denitrifying Bacteria of Wastewater Treatment Systems

Direct Profiling of rRNA in Saline Wastewater Treatment Samples Using an Oligonucleotide Microarray

Contact Info

Product

Resources

About