Microbial activity on the microstructure of bacteria modified mortar

Ghosh, Sujan; Biswas, Mukul; Chattopadhyay, Brajadulal; Mandal, Saroj

doi:10.1016/j.cemconcomp.2009.01.001

Cited by 167 publications

(77 citation statements)

References 10 publications

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“…The bacterium was found to secrete bioremediase-like protein which similarly possessed the property of biosilicification as found in BKH1 and BKH2 bacterial species described earlier [13] [15]. It might, therefore, be inferred that the bioremediase like protein similarly produced new Gehlenite phase within the pores of the mortar matrices which took active role in enhancement of compressive strength and other properties of as prepared bio-concrete material as elaborately described in the previous publications [12] [15] [16]. Due to the variation of composition of the ingredients in concrete/mortar mixture, the compressive or tensile strengths vary to a certain extent (10% maximum).…”

Section: Resultsmentioning

confidence: 82%

Phylogenetic Characterization of BKH3 Bacterium Isolated from a Hot Spring Consortium of Bakreshwar (India) and Its Application

Chaudhuri¹,

Alam²,

Sarkar³

et al. 2016

AiM

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Background: The silica leaching attribute of some of the mystifying bacteria present in the cluster of hot springs (temperatures range 35˚C -80˚C) at Bakreshwar (West Bengal, India, 23˚52'48"N; 87˚22'40") has provided some significant advancements in construction technology when incorporated to the concrete/mortar specimens. The present investigation was designed to isolate other novel bacterial strains from 65˚C hot spring that could have similar or better performance in construction technology. Methods: Soil sample collected from the 65˚C hot spring was inoculated to the culture vials (incubated at 65˚C) containing a specific synthetic growth medium (pH 8.0) to grow the bacterial population anaerobically by degassing the medium with CO2 gas. Subsequent serial dilution techniques were employed to isolate pure culture of a specific bacterial strain. 16S rRNA gene sequence and phylogenetic analysis was carried out to identify the novelty of the isolated bacterial strain. The isolated bacteria were incorporated to the cement sand mixture at various cell concentrations to evaluate the efficacy of the strain in construction technology. Results: The work revealed the presence of a novel bacterial strain (BKH3; GenBank Accession No.: KP 890928) within the same hot spring consortium whose 16S rRNA gene sequence data showed 96% identity with Citrobacter freundii bacterial species. The newly isolated bacteria when incorporated at different cell concentration to the cement/cement-sand mixture were found to possess the similar compressive strength increment property, the cracks repairing ability and the water ingression resistivity. It also reduced the permeability of sulphate ions to the cementitious matrix reflecting the increment of durability of the incorporated material. Conclusions: The enhancement of compressive strength and durability of the as prepared bio-concrete material by using the isolated bacterial strain (BKH3) was due to the silica leaching activity of the bioremediase like pro-* Corresponding author. B. Chaudhuri et al. 454tein secreted by the bacterium. This may open up another vista of utilization of hot spring bacterium for beneficial purposes in construction technology.

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Section: Resultsmentioning

confidence: 82%

Phylogenetic Characterization of BKH3 Bacterium Isolated from a Hot Spring Consortium of Bakreshwar (India) and Its Application

Chaudhuri¹,

Alam²,

Sarkar³

et al. 2016

AiM

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“…Extensive research has been carried out with different species of bacteria in improving the strength and durability of concrete (De Muynck et al 2008;Ghosh et al 2005;Ghosh et al 2009;Yang and Cheng 2013;Jonkers and Schlangen 2007;Jonkers and Schlangen 2008;Wang et al 2012;Chahal et al 2012). Microbiologically induced mineral precipitation improves the strength by about 25% (Ghosh et al 2005) to 33% (Abo-El-Enein et al 2013) according to the reported literature.…”

Section: Introductionmentioning

confidence: 82%

“…Microbiologically induced mineral precipitation improves the strength by about 25% (Ghosh et al 2005) to 33% (Abo-El-Enein et al 2013) according to the reported literature. The reasons for strength improvement as reported (Ghosh et al 2009) are due to a) the growth of filler material within the pores of the cement mortar b) the formation of new phases of silicates and c) uniform distribution of silicate phases and increased Ca/Si ratio within the C-S-H gel of the matrices due to bacterial treatment.…”

Section: Introductionmentioning

confidence: 99%

Investigation of Bacterial Activity on Compressive Strength of Cement Mortar in Different Curing Media

Thiyagarajan

Maheswaran

Mapa

et al. 2016

ACT

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Recently, bacterial concrete is gaining popularity due to the advantages such as self healing property, increased durability, enhanced strength etc. When living organisms are introduced into cementitious materials, many factors are expected to influence their activity. A conducive environment such as temperature, nutrients, pH etc. is necessary for the survival and intended activity of bacteria in concrete. Among all the influential factors, nutrients for the survival of bacteria in cement mortar is considered in this study. In order to supply nutrients for the bacterial growth, three different curing media such as normal water, Luria Bertania broth (controlled nutrients) and wastewater (uncontrolled nutrients) are chosen. Compressive strength are determined at various stages of curing to study the influence of bacterial activity in cement mortar. Further, X ray diffraction and thermo gravimetric analysis are carried out to understand the transformation in hydration phases with the incorporation of bacteria. From the strength study conducted, cubes cured in waste water showed more strength which indicates that the bacterial activity is more in waste water.

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“…Several authors have mentioned increases of 28 d compressive strength with 9 to 25% when mixing bacterial cells into mortar or concrete [19][20][21]. Others mention both positive and negative effects, depending on the bacterial strain, cell concentration or concrete age [22][23].…”

Section: Microbial Caco 3 For Strength Improvementmentioning

confidence: 99%

Application of bacteria in concrete: a critical evaluation of the current status

Belie

2016

RILEM Tech Lett

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Microbially induced carbonate precipitation has been tested over more than a decade as a technique to enhance concrete properties. Mainly bacteria following the pathways of urea decomposition, oxidation of organic acids, or nitrate reduction have been studied for this purpose. For bacteria mixed into fresh concrete, it is difficult to prove that they actively contribute to calcium carbonate precipitation and the effects on concrete strength are variable. Application of bacteria for surface consolidation has been shown to reduce water absorption and increase durability. Microbial self-healing of cracks in concrete shows promising results at the laboratory scale. Especially the use of self-protected mixed cultures opens perspectives for practical application. However, their self-healing efficiency needs to be further proven in larger concrete elements, and under non-ideal conditions. The use of denitrifying cultures for concurrent self-healing and production of corrosion inhibiting nitrites is a promising new strategy.

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Microbial activity on the microstructure of bacteria modified mortar

Cited by 167 publications

References 10 publications

Phylogenetic Characterization of BKH3 Bacterium Isolated from a Hot Spring Consortium of Bakreshwar (India) and Its Application

Phylogenetic Characterization of BKH3 Bacterium Isolated from a Hot Spring Consortium of Bakreshwar (India) and Its Application

Investigation of Bacterial Activity on Compressive Strength of Cement Mortar in Different Curing Media

Application of bacteria in concrete: a critical evaluation of the current status

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