Microbial abundance and community composition in biofilms on in-pipe sensors in a drinking water distribution system

Abstract

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Factors Shaping Young and Mature Bacterial Biofilm Communities in Two Drinking Water Distribution Networks

“…Similarly, the family Nitrosomonadaceae showed higher relative abundance under high monochloramine conditions on Campus B (7.30% for YSB and 9.56% for MPB) when compared to Campus A (0.02% for YSB_A and 2.40% for MPB_A). Nitrosomonadaceae (betaproteobacteria class) are ammonia oxidizers that play an important role in drinking water networks (Cruz et al 2020, Kitajima et al 2020, Pérez et al 2019).…”

“…Traditionally, biofilm formation has been associated with the detection of a deposit on a surface using differential turbidity measurement (DTM) between the clean and colonized probe (Flemming et al 1998). The online WaterWise system was initially developed to detect leakages but has also enabled additional real-time monitoring of water quality parameters, including pH, ORP, temperature, conductivity and turbidity, for the whole DWDS in Singapore (Allen et al 2013, Kitajima et al 2020, PUB 2016). Kitajima et al (2020) suggested that water age and monochloramine drive the community of both biofilm and bulk water samples.…”

“…The online WaterWise system was initially developed to detect leakages but has also enabled additional real-time monitoring of water quality parameters, including pH, ORP, temperature, conductivity and turbidity, for the whole DWDS in Singapore (Allen et al 2013, Kitajima et al 2020, PUB 2016). Kitajima et al (2020) suggested that water age and monochloramine drive the community of both biofilm and bulk water samples. Here we report on the use of these sensors to observe the microbial community dynamics in biofilms from two operational campus drinking water networks, referred to as Testbeds A and B.…”

Nascent nitrifying community response to monochloramine exposure in drinking water

“…The design of functional gene primers to quantify nitrifiers using ddPCR was necessary to overcome the limitations of qPCR in terms of a higher detection limit and sensitivity to amplification. In the survey of bulk water and biofilm communities in the local DWDS network with in-pipe sensors, a discrepancy was observed between the quantitation of total Archaea with ddPCR and the detection of archaeal amoA with qPCR (Kitajima, M. et al 2020).…”

“…In that study, an archaeal 16S rRNA gene primer was used for ddPCR quantification (Yu, Y. et al 2005, Kitajima, M. et al 2020, while the full-length archaeal amoA primer was used for qPCR quantification (Francis, C.A. et al 2005, Kitajima, M. et al 2020.…”