Microarray-based identification of clinically relevant vaginal bacteria in relation to bacterial vaginosis

Dols, Joke; Smit, Pieter W.; Kort, Remco; Reid, Gregor; Schuren, Frank; Tempelman, Hugo; Bontekoe, Tj. Romke; Korporaal, Hans; Boon, Mathilde E.

doi:10.1016/j.ajog.2010.11.012

Cited by 48 publications

(47 citation statements)

References 33 publications

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“…A similar trend was found for HIV-1 RNA shedding in the genital tract of HIV-positive women. These findings are in agreement with the majority of studies that have also investigated these associations (Spear et al, 2008;Mitchell et al, 2009;Dols et al, 2011;Pépin et al, 2011;Schellenberg et al, 2011;Dols et al, 2012;Mitchell et al, 2013), but we are the first to report these associations for multiple STI pathogens and to demonstrate a dose-response relationship. It is important to note that the L. crispatus cluster only contained 11 women, and that the temporality of our findings is unclear because our study was cross-sectional.…”

Section: Discussionsupporting

confidence: 81%

“…Microarray sample preparation, labeling, amplification and hybridization were described previously (Dols et al, 2011;Supplementary Information).…”

Section: Microarray Designmentioning

confidence: 99%

“…The microarray contained 461 DNA hybridization probes targeting microorganisms and 164 positive (16S conserved regions) and negative controls (Supplementary Information) (Dols et al, 2011(Dols et al, , 2012. Of the 251 probes that generated a consistent signal with a signal/background (S/B) ratio 45, 66 16S probes were species-specific, 56 16S probes targeted multiple bacterial species within one genus, 36 16S probes were specific at family or order level, 69 targeted higher taxonomic levels, 5 were groEL probes, 16 were 18S probes and 3 were viral probes.…”

Section: Microarray Designmentioning

confidence: 99%

“…A few exploratory studies have described differences between HIV-positive and HIV-negative women, and most found trends toward decreased lactobacilli and increased bacterial diversity (Spear et al, 2008;Mitchell et al, 2009;Dols et al, 2011;Pépin et al, 2011;Schellenberg et al, 2011;Dols et al, 2012;Mitchell et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Lactobacillus-dominated cervicovaginal microbiota associated with reduced HIV/STI prevalence and genital HIV viral load in African women

Tsivtsivadze²,

et al. 2014

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Cervicovaginal microbiota not dominated by lactobacilli may facilitate transmission of HIV and other sexually transmitted infections (STIs), as well as miscarriages, preterm births and sepsis in pregnant women. However, little is known about the exact nature of the microbiological changes that cause these adverse outcomes. In this study, cervical samples of 174 Rwandan female sex workers were analyzed cross-sectionally using a phylogenetic microarray. Furthermore, HIV-1 RNA concentrations were measured in cervicovaginal lavages of 58 HIV-positive women among them. We identified six microbiome clusters, representing a gradient from low semi-quantitative abundance and diversity dominated by Lactobacillus crispatus (cluster R-I, with R denoting 'Rwanda') and L. iners (R-II) to intermediate (R-V) and high abundance and diversity (R-III, R-IV and R-VI) dominated by a mixture of anaerobes, including Gardnerella, Atopobium and Prevotella species. Women in cluster R-I were less likely to have HIV (P ¼ 0.03), herpes simplex virus type 2 (HSV-2; Po0.01), and high-risk human papillomavirus (HPV; Po0.01) and had no bacterial STIs (P ¼ 0.15). Statistically significant trends in prevalence of viral STIs were found from low prevalence in cluster R-I, to higher prevalence in clusters R-II and R-V, and highest prevalence in clusters R-III/R-IV/R-VI. Furthermore, only 10% of HIV-positive women in clusters R-I/R-II, compared with 40% in cluster R-V, and 42% in clusters R-III/ R-IV/R-VI had detectable cervicovaginal HIV-1 RNA (P trend ¼ 0.03). We conclude that L. crispatusdominated, and to a lesser extent L. iners-dominated, cervicovaginal microbiota are associated with a lower prevalence of HIV/STIs and a lower likelihood of genital HIV-1 RNA shedding.

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Section: Discussionsupporting

confidence: 81%

“…Microarray sample preparation, labeling, amplification and hybridization were described previously (Dols et al, 2011;Supplementary Information).…”

Section: Microarray Designmentioning

confidence: 99%

Section: Microarray Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Lactobacillus-dominated cervicovaginal microbiota associated with reduced HIV/STI prevalence and genital HIV viral load in African women

Tsivtsivadze²,

et al. 2014

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“…Although hybridization studies have targeted only a few well-known pathogens, the results are consistent with those of other studies that showed that the presence of one or more specific pathogens correlates well with a diagnosis of BV. The search for molecular tools for the diagnosis of BV has also included the use of microarray technology (48), although the utility of such assays for routine diagnostic purposes remains to be proven. Commercially available, FDA-approved assays that utilize molecular methods of detection are usually not directed at BV alone but include probes for other known causes of vaginitis, such as N. gonorrhoeae, Trichomonas vaginalis, Chlamydia trachomatis, and G. vaginalis.…”

Section: Non-culture-based Methods For Diagnosis Of Bvmentioning

confidence: 99%

The Human Microbiome during Bacterial Vaginosis

2016

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SUMMARYBacterial vaginosis (BV) is the most commonly reported microbiological syndrome among women of childbearing age. BV is characterized by a shift in the vaginal flora from the dominantLactobacillusto a polymicrobial flora. BV has been associated with a wide array of health issues, including preterm births, pelvic inflammatory disease, increased susceptibility to HIV infection, and other chronic health problems. A number of potential microbial pathogens, singly and in combinations, have been implicated in the disease process. The list of possible agents continues to expand and includes members of a number of genera, includingGardnerella,Atopobium,Prevotella,Peptostreptococcus,Mobiluncus,Sneathia,Leptotrichia,Mycoplasma, and BV-associated bacterium 1 (BVAB1) to BVAB3. Efforts to characterize BV using epidemiological, microscopic, microbiological culture, and sequenced-based methods have all failed to reveal an etiology that can be consistently documented in all women with BV. A careful analysis of the available data suggests that what we term BV is, in fact, a set of common clinical signs and symptoms that can be provoked by a plethora of bacterial species with proinflammatory characteristics, coupled to an immune response driven by variability in host immune function.

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PCR‐based identification of eight lactobacillus species and 18 hr‐HPV genotypes in fixed cervical samples of south african women at risk of HIV and BV

Dols

Reid

Kort³

et al. 2011

Diagnostic Cytopathology

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Vaginal lactobacilli assessed by PCR-based microarray and PCR-based genotyping of HPV in South African women at risk for HIV and BV. Vaginal lactobacilli can be defined by microarray techniques in fixed cervical samples of South African women. Cervical brush samples suspended in the coagulant fixative BoonFix of one hundred women attending a health centre for HIV testing in South Africa were available for this study. In the Ndlovu Medical Centre in Elandsdoorn, South Africa, identification of 18 hr-HPV genotypes was done using the INNO-LiPA method. An inventory of lactobacilli organisms was performed using microarray technology. On the basis of the Lactobacillus and Lactobacillus biofilm scoring, the cases were identified as Leiden bacterial vaginosis (BV) negative (BV-; n = 41), Leiden BV intermediate (BV±; n = 25), and Leiden BV positive (BV+; n = 34). Fifty-one women were HIV positive and 49 HIV negative. Out of the 51 HIV positive women, 35 were HPV infected. These 51 HIV positive women were frequently infected with HPV16 and HPV18. In addition, HPV35, HPV52, HPV33, and HPV66 were often detected in these samples. Lactobacillus salivarius and Lactobacillus iners were the most prevalent lactobacilli as established by the microarray technique. In women with HPV infection, the prevalence of Lactobacillus crispatus was significantly reduced. In both HIV and HPV infection, a similar (but not identical) shift in the composition of the lactobacillus flora was observed. We conclude that there is a shift in the composition of vaginal lactobacilli in HIV-infected women. Because of the prominence of HPV35, HPV52, HPV33, and HPV66, vaccination for exclusively HPV16 and HPV18 might be insufficient in South African HIV+ women.

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Microarray-based identification of clinically relevant vaginal bacteria in relation to bacterial vaginosis

Cited by 48 publications

References 33 publications

Lactobacillus-dominated cervicovaginal microbiota associated with reduced HIV/STI prevalence and genital HIV viral load in African women

Lactobacillus-dominated cervicovaginal microbiota associated with reduced HIV/STI prevalence and genital HIV viral load in African women

The Human Microbiome during Bacterial Vaginosis

PCR‐based identification of eight lactobacillus species and 18 hr‐HPV genotypes in fixed cervical samples of south african women at risk of HIV and BV

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