Microarray-based gene expression profiles of silkworm brains

Gan, Ling; Liu, Xilong; Zhang, Xiang; He, Ningjia

doi:10.1186/1471-2202-12-8

Cited by 11 publications

(9 citation statements)

References 53 publications

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“…Neuropeptides are a kind of bioactive substance that exist in nervous tissue and play key roles in regulating the function of the nervous system. It has been reported that 51 neuropeptides are expressed in the silkworm brain, such as neuropeptide F1, short neuropeptide F, bombyxin, PTTH, corazonin, and so on . PTTH is a homodimeric glycoprotein; our qRT-PCR results showed that its coding gene was highly expressed at wandering stage (Figure F), which was consistent with previous results .…”

Section: Discussionsupporting

confidence: 92%

Integrative Proteomics and Metabolomics Analysis of Insect Larva Brain: Novel Insights into the Molecular Mechanism of Insect Wandering Behavior

Wang

Hou

et al. 2015

J. Proteome Res.

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Before metamorphosis, most holometabolous insects, such as the silkworm studied here, undergo a special phase called the wandering stage. Insects in this stage often display enhanced locomotor activity (ELA). ELA is vital because it ensures that the insect finds a safe and suitable place to live through the pupal stage. The physiological mechanisms of wandering behavior are still unclear. Here, we integrated proteomics and metabolomics approaches to analyze the brain of the lepidopteran insect, silkworm, at the feeding and wandering stages. Using LC-MS/MS and GC-MS, in all we identified 3004 proteins and 37 metabolites at these two stages. Among them, 465 proteins and 22 metabolites were changed. Neural signal transduction proteins and metabolites, such as neurofilament, dopaminergic synapse related proteins, and glutamic acid, were significantly altered, which suggested that active neural conduction occurred in the brain at the wandering stage. We also found decreased dopamine degradation at the wandering stage. The proposed changes in active neural conduction and increased dopamine concentration might induce ELA. In addition, proteins involved in the ubiquitin proteasome system and lysosome pathway were upregulated, revealing that the brain experiences morphological remodeling during metamorphosis. These findings yielded novel insights into the molecular mechanism underlying insect wandering behavior.

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Section: Discussionsupporting

confidence: 92%

Integrative Proteomics and Metabolomics Analysis of Insect Larva Brain: Novel Insights into the Molecular Mechanism of Insect Wandering Behavior

Wang

Hou

et al. 2015

J. Proteome Res.

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“…Totally, 15 neuropeptides and 66 neuropeptide receptors were identified from head of adult P. flammans , which was different with other lepidopteran species 44 – 47 and should partly be relevant with their differences in sample physiological status. For example, in B. mori , 32 neuropeptide genes and 6 neuropeptide-like precursor genes were identified from larval and pupal brain 45 . In C. suppressalis , 43 neuropeptide precursors and 51 putative neuropeptide G protein-coupled receptors were identified the fifth instar larval central nervous system including brain, suboeophageal ganglion, thoracic ganglion, and the abdominal ganglion 46 .…”

Section: Discussionmentioning

confidence: 99%

Identification of neuropeptides and neuropeptide receptor genes in Phauda flammans (Walker)

Wang

et al. 2022

Sci Rep

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Neuropeptides and neuropeptide receptors are crucial regulators to insect physiological processes. The 21.0 Gb bases were obtained from Illumina sequencing of two libraries representing the female and male heads of Phauda flammans (Walker) (Lepidoptera: Phaudidae), which is a diurnal defoliator of ficus plants and usually outbreaks in the south and south-east Asia, to identify differentially expressed genes, neuropeptides and neuropeptide receptor whose tissue expressions were also evaluated. In total, 99,386 unigenes were obtained, in which 156 up-regulated and 61 down-regulated genes were detected. Fifteen neuropeptides (i.e., F1b, Ast, NP1, IMF, Y, BbA1, CAP2b, NPLP1, SIF, CCH2, NP28, NP3, PDP3, ARF2 and SNPF) and 66 neuropeptide receptor genes (e.g., A2-1, FRL2, A32-1, A32-2, FRL3, etc.) were identified and well-clustered with other lepidopteron. This is the first sequencing, identification neuropeptides and neuropeptide receptor genes from P. flammans which provides valuable information regarding the molecular basis of P. flammans.

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“…A burgeoning literature has linked changes in brain gene expression to changes in behavior in several species (20,21,23,24,39,45,74,79,91,100,103,117,122), but further work is needed.…”

Section: Is a Close Relationship Between Neurogenomic State And Behavmentioning

confidence: 99%

Understanding the Relationship Between Brain Gene Expression and Social Behavior: Lessons from the Honey Bee

2012

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Behavior is a complex phenotype that is plastic and evolutionarily labile. The advent of genomics has revolutionized the field of behavioral genetics by providing tools to quantify the dynamic nature of brain gene expression in relation to behavioral output. The honey bee Apis mellifera provides an excellent platform for investigating the relationship between brain gene expression and behavior given both the remarkable behavioral repertoire expressed by members of its intricate society and the degree to which behavior is influenced by heredity and the social environment. Here, we review a linked series of studies that assayed changes in honey bee brain transcriptomes associated with natural and experimentally induced changes in behavioral state. These experiments demonstrate that brain gene expression is closely linked with behavior, that changes in brain gene expression mediate changes in behavior, and that the association between specific genes and behavior exists over multiple timescales, from physiological to evolutionary.

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Microarray-based gene expression profiles of silkworm brains

Cited by 11 publications

References 53 publications

Integrative Proteomics and Metabolomics Analysis of Insect Larva Brain: Novel Insights into the Molecular Mechanism of Insect Wandering Behavior

Integrative Proteomics and Metabolomics Analysis of Insect Larva Brain: Novel Insights into the Molecular Mechanism of Insect Wandering Behavior

Identification of neuropeptides and neuropeptide receptor genes in Phauda flammans (Walker)

Understanding the Relationship Between Brain Gene Expression and Social Behavior: Lessons from the Honey Bee

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