Micro-Optical Sectioning Tomography to Obtain a High-Resolution Atlas of the Mouse Brain

Li, Anan; Gong, Hui; Zhang, Bin; Wang, Qingdi; Cheng, Yan; Wu, Jingpeng; Liu, Qian; Zeng, Shaoqun; Luo, Qingming

doi:10.1126/science.1191776

Cited by 458 publications

(402 citation statements)

References 15 publications

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“…Studies of cortical connection structure [47][48][49] and function could be extended to animals that model disease. Investigators have begun to develop hardware to stimulate circuits that were lost due to ischemic damage 50 .…”

Section: Relation Of Activity Propagation To Axonal Projectionsmentioning

confidence: 99%

Spontaneous cortical activity alternates between motifs defined by regional axonal projections

et al. 2013

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In lightly anaesthetized or awake adult mice using millisecond timescale voltage sensitive dye imaging, we show that a palette of sensory-evoked and hemisphere-wide activity motifs are represented in spontaneous activity. These motifs can reflect multiple modes of sensory processing including vision, audition, and touch. Similar cortical networks were found with direct cortical activation using channelrhodopsin-2. Regional analysis of activity spread indicated modality specific sources such as primary sensory areas, and a common posterior-medial cortical sink where sensory activity was extinguished within the parietal association area, and a secondary anterior medial sink within the cingulate/secondary motor cortices for visual stimuli. Correlation analysis between functional circuits and intracortical axonal projections indicated a common framework corresponding to long-range mono-synaptic connections between cortical regions. Maps of intracortical mono-synaptic structural connections predicted hemisphere-wide patterns of spontaneous and sensory-evoked depolarization. We suggest that an intracortical monosynaptic connectome shapes the ebb and flow of spontaneous cortical activity.

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Section: Relation Of Activity Propagation To Axonal Projectionsmentioning

confidence: 99%

Spontaneous cortical activity alternates between motifs defined by regional axonal projections

et al. 2013

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“…A three-dimensional structural data set of a Golgi-stained whole brain at the neurite level has been obtained to explore connectional groups and functional groups in the basic structure and function of neural circuitry [2] . The dominant view that a saliency map is generated in the parietal cortex has been challenged because a bottomup saliency map is created in V1 [1] .…”

Section: Neural Circuit Research In Emotion and Memorymentioning

confidence: 99%

Grand Research Plan for Neural Circuits of Emotion and Memory — Current status of neural circuit studies in China

2013

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During recent years, major advances have been made in neuroscience, i.e., asynchronous release, threedimensional structural data sets, saliency maps, magnesium in brain research, and new functional roles of long non-coding RNAs. Especially, the development of optogenetic technology provides access to important information about relevant neural circuits by allowing the activation of specific neurons in awake mammals and directly observing the resulting behavior. The Grand Research Plan for Neural Circuits of Emotion and Memory was launched by the National Natural Science Foundation of China. It takes emotion and memory as its main objects, making the best use of cutting-edge technologies from medical science, life science and information science. In this paper, we outline the current status of neural circuit studies in China and the technologies and methodologies being applied, as well as studies related to the impairments of emotion and memory. In this phase, we are making efforts to repair the current deficiencies by making adjustments, mainly involving four aspects of core scientific issues to investigate these circuits at multiple levels. Five research directions have been taken to solve important scientific problems while the Grand Research Plan is implemented. Future research into this area will be multimodal, incorporating a range of methods and sciences into each project. Addressing these issues will ensure a bright future, major discoveries, and a higher level of treatment for all affected by debilitating brain illnesses.

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“…Thus far, providing an image at organelle-level resolution of a whole organ has remained a challenge. Recently, optical microscopy assisted by chemical clearing or mechanical sectioning of tissue has been proven as a powerful technique to overcome this dilemma [1][2][3] . However, this type of techniques requires lengthy special preparation of the tissue specimen, hindering their applicability in life sciences.…”

Section: Introductionmentioning

confidence: 99%

Whole-organ atlas imaged by label-free high-resolution photoacoustic microscopy assisted by a microtome

Wong

Zhang

Hsu

et al. 2018

Photons Plus Ultrasound: Imaging and Sensing 2018

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In biomedical imaging, all optical techniques face a fundamental trade-off between spatial resolution and tissue penetration. Therefore, obtaining an organelle-level resolution image of a whole organ has remained a challenging and yet appealing scientific pursuit. Over the past decade, optical microscopy assisted by mechanical sectioning or chemical clearing of tissue has been demonstrated as a powerful technique to overcome this dilemma, one of particular use in imaging the neural network. However, this type of techniques needs lengthy special preparation of the tissue specimen, which hinders broad application in life sciences. Here, we propose a new label-free three-dimensional imaging technique, named microtomy-assisted photoacoustic microscopy (mPAM), for potentially imaging all biomolecules with 100% endogenous natural staining in whole organs with high fidelity. We demonstrate the first label-free mPAM, using UV light for label-free histology-like imaging, in whole organs (e.g., mouse brains), most of them formalin-fixed and paraffin-or agarose-embedded for minimal morphological deformation. Furthermore, mPAM with dual wavelength illuminations is also employed to image a mouse brain slice, demonstrating the potential for imaging of multiple biomolecules without staining. With visible light illumination, mPAM also shows its deep tissue imaging capability, which enables less slicing and hence reduces sectioning artifacts. mPAM could potentially provide a new insight for understanding complex biological organs.

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Micro-Optical Sectioning Tomography to Obtain a High-Resolution Atlas of the Mouse Brain

Cited by 458 publications

References 15 publications

Spontaneous cortical activity alternates between motifs defined by regional axonal projections

Spontaneous cortical activity alternates between motifs defined by regional axonal projections

Grand Research Plan for Neural Circuits of Emotion and Memory — Current status of neural circuit studies in China

Whole-organ atlas imaged by label-free high-resolution photoacoustic microscopy assisted by a microtome

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