Micro and ultrastructural changes monitoring during decellularization for the generation of a biocompatible liver

Ahmed, Ebtehal; Saleh, Tarek M.; Yu, Lejun; Kwak, Hyung Woo; Kim, Byeong–Moo; Park, Kyung-Mee; Lee, Yun‐Suk; Kang, Byung‐Jae; Choi, Ki‐Young; Kang, Kyung‐Sun; Woo, Heung‐Myong

doi:10.1016/j.jbiosc.2019.02.007

Cited by 27 publications

(22 citation statements)

References 30 publications

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“…We principally used Tx detergent for decellularization, since it is a proven gentle decellularizing agent (Gilbert, Sellaro, & Badylak, 2006). However, it must be used in conjunction with other detergents to produce efficiently decellularized scaffolds (Ahmed et al, 2019). Our results confirmed that the Tx/DNase protocol after step 5 is very effective for decellularizing rat liver by removing all cellular remnants microscopically and ultrastructurally without destroying the native ECM architecture or depleting the ECM components.…”

Section: Discussionmentioning

confidence: 99%

Decellularized extracellular matrix‐rich hydrogel–silver nanoparticle mixture as a potential treatment for acute liver failure model

Ahmed

Saleh

et al. 2020

J Biomedical Materials Res

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Acute liver failure (ALF) occurs due to severe liver damage that triggers rapid loss of normal liver function. Here, we investigate the usefulness of an injectable liver extracellular matrix (LECM)-rich hydrogel generated from an optimized decellularization protocol incorporated with silver nanoparticles (AgNPs) as a promising therapy for ALF. First, we optimized a non-destructive protocol for rat liver decellularization to obtain ECM-rich well-preserved scaffold. Then, LECM hydrogel generated from two commonly used decellularization protocols were compared by LECM hydrogel obtained from our optimized protocol. The ALF model was induced by an intraperitoneal (IP) thioacetamide (TAA) injection followed by the IP injection of LECM hydrogel, collagen-AgNP mixture, or LECM hydrogel-AgNP mixture. LECM-rich scaffold and hydrogel were successfully obtained using our optimized decellularization protocol. Use of the LECM hydrogel-AgNP mixture to treat TAA-induced ALF greatly improved liver injury and histological liver regeneration. Interleukin-6 and transforming growth factor-beta expressions were significantly reduced, while albumin, hepatocyte growth factor, and Ki67-positive cells were highly expressed. Moreover, aspartate transaminase and alanine transaminase plasma levels and liver homogenate nitric oxide level were significantly lowered. In conclusion, the LECM hydrogel-AgNP mixture has potential efficient therapeutic and regenerative effects on TAA-induced liver injury.

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Section: Discussionmentioning

confidence: 99%

Decellularized extracellular matrix‐rich hydrogel–silver nanoparticle mixture as a potential treatment for acute liver failure model

Ahmed

Saleh

et al. 2020

J Biomedical Materials Res

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“…The most promising and interesting source of liver scaffolds is native hepatic tissue [87][88][89]. Decellularization of the native livers of lab animals, pigs, and humans has been tested to determine the most suitable and applicable way to generate transplantable bioengineered liver scaffolds [85,[90][91][92]. Lab animal liver decellularization was studied as a preliminary step to evaluate the gentlest decellularization protocol by characterizing their biochemical components and structural properties as compared with native tissues before testing in larger animals or human tissue [90] (Fig.…”

Section: Bioengineered Liver Scaffolds and Their Transplantationmentioning

confidence: 99%

“…Various decellularization protocols have been described for generating a cellular hepatic scaffold. Washing out of cells is mainly performed using chemical detergents such as sodium dodecyl sulfate (SDS) and Triton-X-100, endonuclease enzymes such as DNases, or different combinations of chemicals and enzymes with agitation for liver slices, or by either continuous or pressure-controlled whole liver perfusions [82,85,90,92,[97][98][99]. Decellularization under oscillating pressure has shown better results in terms of perfusion homogeneity and acellular tissue integrity [85,92,98,100].…”

Section: Bioengineered Liver Scaffolds and Their Transplantationmentioning

confidence: 99%

Cell Therapy and Bioengineering in Experimental Liver Regenerative Medicine: In Vivo Injury Models and Grafting Strategies

et al. 2021

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Purpose of Review To describe experimental liver injury models used in regenerative medicine, cell therapy strategies to repopulate damaged livers and the efficacy of liver bioengineering. Recent Findings Several animal models have been developed to study different liver conditions. Multiple strategies and modified protocols of cell delivery have been also reported. Furthermore, using bioengineered liver scaffolds has shown promising results that could help in generating a highly functional cell delivery system and/or a whole transplantable liver. Summary To optimize the most effective strategies for liver cell therapy, further studies are required to compare among the performed strategies in the literature and/or innovate a novel modifying technique to overcome the potential limitations. Coating of cells with polymers, decellularized scaffolds, or microbeads could be the most appropriate solution to improve cellular efficacy. Besides, overcoming the problems of liver bioengineering may offer a radical treatment for end-stage liver diseases.

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“…[8][9][10] For developing scaffolds, both proper materials and suitable techniques, according to which they are fabricated, should be implemented so that they have essential properties. 11 Regarding this, three types of scaffolds, including implanted scaffolds, [12][13][14] injected hydrogels, [15][16][17] and acellular scaffolds, [18][19][20][21][22] are employed. The first and second types of scaffolds have benefits and drawbacks such as desirable mechanical strength and inadequate biological properties, respectively.…”

Section: Introductionmentioning

confidence: 99%

Cardiac tissue engineering, biomaterial scaffolds, and their fabrication techniques

Roshandel

Dorkoosh

2021

Polymers for Advanced Techs

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Cardiovascular diseases are the first cause of mortality around the globe. One of the promising treatment procedures is regenerating or repairing the heart tissues.Regarding this, in tissue engineering, there are variety of methods that researchers could use for scaffolds fabrication. In this review paper, some of the biomaterials and techniques, by means of which biomaterial scaffolds can be generated, are explained. Furthermore, some recent studies, in which scaffolds have been used for the cardiac tissues, have been presented, which can lead researchers to pursue more precise studies.

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Micro and ultrastructural changes monitoring during decellularization for the generation of a biocompatible liver

Cited by 27 publications

References 30 publications

Decellularized extracellular matrix‐rich hydrogel–silver nanoparticle mixture as a potential treatment for acute liver failure model

Decellularized extracellular matrix‐rich hydrogel–silver nanoparticle mixture as a potential treatment for acute liver failure model

Cell Therapy and Bioengineering in Experimental Liver Regenerative Medicine: In Vivo Injury Models and Grafting Strategies

Cardiac tissue engineering, biomaterial scaffolds, and their fabrication techniques

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