micrIO: an open-source autosampler and fraction collector for automated microfluidic input–output

Longwell, Scott A.; Fordyce, Polly M.

doi:10.1039/c9lc00512a

Cited by 25 publications

(23 citation statements)

References 57 publications

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“…To reduce cost and facilitate custom applications, a number of open-source fraction collectors have been developed, e.g. (Caputo et al, 2020;Longwell and Fordyce, 2020) . These devices, while less expensive, continue to rely on complex engineering designs and parts that may be difficult to source and manufacture, thus driving costs higher, lengthening the assembly process, and complicating operation.…”

Section: Introductionmentioning

confidence: 99%

“…The colosseum fraction collector can be assembled in less than an hour and costs $67.02. Unlike the micrIO (Longwell and Fordyce, 2020) , which is built from parts of a salvaged Illumina Genome Analyzer that costs $1500, the colosseum fraction collector uses off-the-shelf and 3D-printed parts (Supplementary Table 2). The LEGO MINDSTORM fraction collector (Caputo et al, 2020) costs $500, and while it uses more commonly available components, it still requires cutting and bending of steel C-channel.…”

Section: Introductionmentioning

confidence: 99%

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Low-cost, scalable, and automated fluid sampling for fluidics applications

et al. 2021

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Low-cost, scalable, and automated fluid sampling for fluidics applications

et al. 2021

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“…2b), similar to manual pipetting and other robots. 7,10,[27][28][29][30] The resulting volumes varied from the target volume by +/-2 μ L regardless of the target volume, suggesting droplet retention at the pipet tip rather than pump inaccuracies as the source of this variation (see below). When testing the impact of pumping speed on accuracy, resulting volumes were within 1-2% of the target volume for 10, 25, and 50 µL/s.…”

Section: Robust Media Exchange In Multi-well Plates Via Automated Pipettingmentioning

confidence: 98%

Open-source personal pipetting robots with live-cell incubation and microscopy compatibility

Dettinger

Kull

Arekatla

et al. 2021

Preprint

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Liquid handling robots have the potential to automate many procedures in life sciences. However, they are not in widespread use in academic settings, where funding, space and maintenance specialists are usually limiting. In addition, current robots require lengthy programming by specialists and are incompatible with most academic laboratories with constantly changing small-scale projects. Here, we present the Pipetting Helper Imaging Lid (PHIL), an inexpensive, small, open-source personal liquid handling robot. It is designed for inexperienced users, with self-production from cheap commercial and 3D-printable components and custom control software. PHIL successfully automated pipetting for e.g. tissue immunostainings and stimulations of live stem and progenitor cells during time-lapse microscopy. PHIL is cheap enough for any laboratory member to have their own personal pipetting robot(s), and enables users without programming skills to easily automate a large range of experiments.

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“…So far, there have been no reports of 3D printed autosamplers. In many currently available autosamplers, each sample probe/needle is driven by three orthogonal linear motion stages to obtain translational freedom in all three dimensions [11]. Such autosamplers can be used to access large number of vials, but are expensive and have large footprints.…”

Section: Introductionmentioning

confidence: 99%

3-D Printed Analytical Platform for Automation of Fluid Manipulation Applied to Leaky Waveguide Biosensors

Goddard

2021

IEEE Trans. Instrum. Meas.

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Automation of fluid manipulation is needed to increase throughput and reproducibility of experiments while reducing the time spent by researchers on performing repetitive tasks. Current solutions are however bulky and expensive, and hence not suited to enable automation in academic laboratories. This work therefore reports a low cost, modular and programmable analytical platform comprising of 3D printed autosampler and peristaltic pump with footprints of 248 mm by 243 mm and 104 mm by 112 mm, respectively. The autosampler consisted of four samples probes/needles that can be driven independently to any of the available solution vials placed along the circumference of a circle. The 3D printed autosampler developed in this work can access higher number of solution vials independently and is lower cost, but accommodates fewer number of total solution vials, than commercial devices. The autosampler and peristaltic pump were applied to determine (1) refractive index sensitivity, (2) porosity, and (3) sensing capability of leaky waveguides (LWs) where the readout instrument was also 3D printed. We demonstrated that the automation of fluids enabled by the analytical platform improved the accuracy of results by separating the effect of temperature drifts from analyte solutions from the output of LWs. This work shows that 3D printed instrumentation can provide results comparable with standard laboratory versions at much lower cost, making it ideal for use in resource-limited settings, and with the possibility of customization as experimental needs change.

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micrIO: an open-source autosampler and fraction collector for automated microfluidic input–output

Abstract: MicrIO is a low-cost, open-source hardware and software solution for automated sample input/output, bridging the gap between microfluidic devices and standard multiwell plates.

Cited by 25 publications

References 57 publications

Low-cost, scalable, and automated fluid sampling for fluidics applications

Low-cost, scalable, and automated fluid sampling for fluidics applications

Open-source personal pipetting robots with live-cell incubation and microscopy compatibility

3-D Printed Analytical Platform for Automation of Fluid Manipulation Applied to Leaky Waveguide Biosensors

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