Miconazole and terbinafine induced reactive oxygen species accumulation and topical toxicity in human keratinocytes

Lam, P. L.; Wong, Megan; Hung, Ling-Chien; Yung, Lai Hang; Tang, Johnny; Lam, Kim Hung; Chung, Po-yee; Wong, Wai Yeung; Ho, Y.-W.; Wong, Raymond Pui On; Gambari, Roberto; Chui, Chung‐Hin

doi:10.1080/01480545.2020.1778019

Cited by 18 publications

(13 citation statements)

References 28 publications

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“…FLZ may also induce an increase in ROS and generate DNA damage [ 28 ]. In turn, MCZ (12.5 µg/mL) stimulates the production of ROS in human keratinocytes (HaCaT), which may induce oxidative stress and cause cell death [ 29 ]. In addition, MCZ inhibits the growth of bladder cancer cells by activating extrinsic and intrinsic apoptotic pathways [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Nanocarriers of Miconazole or Fluconazole: Effects on Three-Species Candida Biofilms and Cytotoxic Effects In Vitro

Caldeirão

Araujo

Arias

et al. 2021

JoF

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The contribution of different Candida species in oral fungal infections has stimulated the search for more effective therapies. This study assessed the antibiofilm effects of nanocarriers of miconazole (MCZ) or fluconazole (FLZ) on Candida biofilms, and their cytotoxic effects on murine fibroblasts. Three-species biofilms (Candida albicans/Candida glabrata/Candida tropicalis) were formed on 96-well plates, and they were treated with nanocarriers (iron oxide nanoparticles coated with chitosan—“IONPs-CS”) of MCZ or FLZ at 39/78/156 µg/mL; antifungals alone at 156 µg/mL and artificial saliva were tested as positive and negative controls, respectively. Biofilms were analyzed by colony forming units (CFU), biomass, metabolic activity, and structure/viability. The cytotoxicity (L929 cells) of all treatments was determined via 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) reduction assay. Data were submitted to one- or two-way ANOVA, followed by Tukey’s or Fisher LSD’s tests (p < 0.05). IONPs-CS-MCZ at 78 µg/mL promoted similar antibiofilm and cytotoxic effects compared with MCZ at 156 µg/mL. In turn, IONPs-CS-FLZ at 156 µg/mL was overall the most effective FLZ antibiofilm treatment, surpassing the effects of FLZ alone; this nanocarrier was also less cytotoxic compared with FLZ alone. It can be concluded that both nanocarriers are more effective alternatives to fight Candida biofilms compared with their respective positive controls in vitro, being a promising alternative for the treatment of oral fungal infections.

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Section: Discussionmentioning

confidence: 99%

Nanocarriers of Miconazole or Fluconazole: Effects on Three-Species Candida Biofilms and Cytotoxic Effects In Vitro

Caldeirão

Araujo

Arias

et al. 2021

JoF

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“…•− ), hydrogen peroxide (H 2 O 2 ) and hydroxyl radicals ( • OH) [25]. In insects, ROS are involved in immune defense reactions against a broad range of pathogens.…”

Section: Discussionmentioning

confidence: 99%

“…The primary function of NADPH oxidase is to produce ROS by transferring an electron from NADPH to oxygen. ROS are a collection of highly reactive molecules containing molecular oxygen (O 2 ), superoxide anion (O 2 •− ), hydrogen peroxide (H 2 O 2 ) and hydroxyl radicals ( • OH) [ 25 ]. In insects, ROS are involved in immune defense reactions against a broad range of pathogens.…”

Section: Discussionmentioning

confidence: 99%

NADPH Oxidase 5 Is Essential for Molting and Oviposition in a Rice Planthopper Nilaparvata lugens

Peng

Dai

Yang

et al. 2020

Insects

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The brown planthopper Nilaparvata lugens is a typical monophagous insect herbivore that feeds exclusively on rice sap. This insect pest causes serious damage to rice crops throughout East Asian countries. Chemical control remains the first choice for managing N. lugens populations; however, the use of insecticides has given rise to planthopper resurgence and additional environmental risks. Nilaparvata lugens is a model insect of Hemiptera because its whole genome sequence has been elucidated and is susceptible to RNA interference. In this study, our findings revealed that a superoxide-generating gene, NADPH oxidase 5 (Nox5), is essential for molting and oviposition in a Hemipteran insect Nilaparvata lugens. Knockdown of Nox5 transcript levels by RNA interference in 2nd–5th-instar nymphs results in significantly lethal deficits in the molting transitions from nymph–nymph and nymph–adult. Nox5 knockdown leads to a reduction of hydrogen peroxide in female ovaries and failure of oviposition from the insect ovipositor into the rice leaf sheath. Here, we provide in vivo evidence demonstrating that Nox5 is a key enzyme for regulating molting and oviposition in this insect species.

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“…Acting as a structural analogue of squalene, terbinafine causes the accumulation of this unsaturated hydrocarbon, and a decrease in ergosterol in the fungal cell membrane. The accumulation of toxic amounts of squalene result in the death of the fungal cell [10].…”

Section: Antimetabolitementioning

confidence: 99%

A short review on liquisolid technology in anti-fungal drugs

Sharma¹,

Kaur²,

Singh³

et al. 2022

WJPS

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This article is based on the mode of action of antifungals and their mechanisms of action and approach for the treatment of fungal diseases like Aspergillosis, Candidemia, Fusariosis, and coccidioidomycosis. There are many different types of mechanisms contribute to the development of resistance to antifungals. The mechanisms include alteration in the drug target, sterol biosynthesis, the reduction in the intercellular concentration of target enzyme, and to make too many copies of the substance of the antifungal drug target.

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Miconazole and terbinafine induced reactive oxygen species accumulation and topical toxicity in human keratinocytes

Cited by 18 publications

References 28 publications

Nanocarriers of Miconazole or Fluconazole: Effects on Three-Species Candida Biofilms and Cytotoxic Effects In Vitro

Nanocarriers of Miconazole or Fluconazole: Effects on Three-Species Candida Biofilms and Cytotoxic Effects In Vitro

NADPH Oxidase 5 Is Essential for Molting and Oviposition in a Rice Planthopper Nilaparvata lugens

A short review on liquisolid technology in anti-fungal drugs

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