Método para avaliação e pesquisa da atividade antimicrobiana de produtos de origem natural

Packer, Janaina F.; Luz, Marisa M.S. da

doi:10.1590/s0102-695x2007000100019

Cited by 77 publications

(54 citation statements)

References 30 publications

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“…Moreover, consumers have become interested in using natural products that are less aggressive than synthetic ones. Thus, the cosmetic industry is seeking alternative products of natural origin and avoiding the use of synthetic ingredients (Packer and Da Luz, 2007). In this context, it is important to search for new active substances with potential cosmetic value.…”

Section: Introductionmentioning

confidence: 99%

“…The huge Brazilian biodiversity furnishes a wide range of plant secondary metabolites that possess activities sought by the cosmetic industry. The flavonoids are among the secondary metabolites extracted from plants with great cosmetic potential because they have antioxidant, anti-inflammatory, antibacterial and other activities (Packer and Da Luz, 2007;Perruchon, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Development of a Topical Formulation Containing S. Lutea Extract: Stability, In Vitro Studies and Cutaneous Permeation

Isaac¹,

Chiari²,

Miglioli³

et al. 2012

J. App. Pharm. Sci.

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Flavonoids have been widely incorporated into cosmetic and dermatological formulations, affording benefits such as antioxidant action, improved skin tone and fewer lines and wrinkles. Brazil has a huge biodiversity, with one of the richest flora in the world, and existing studies justify the quest for greater research efforts in this area. The cajazeira (Spondias lutea L.), a plant of the Anacardiaceae family from tropical America, is widely disseminated in Brazil. This plant was chosen because of the presence of flavonoids that exhibit antioxidant activity. The purpose of this research was to develop a stable topical formulation containing Spondias lutea extract with the aim of preventing skin diseases caused by UV radiation. Hydro ethanolic extract of Spondias lutea fruit was prepared and assayed for its the flavonoids content. The antioxidant activity was estimated by DPPH and superoxide assay. The physicochemical stability and skin permeation of the cream containing 8% (w/w) of extract were assessed. The results showed that the S. lutea extract possessed antioxidant activity, and that it is possible to obtain a stable cosmetic containing the extract, which is able to penetrate the skin. Thus, it is possible to use this extract to produce an anti-aging cosmetic.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a Topical Formulation Containing S. Lutea Extract: Stability, In Vitro Studies and Cutaneous Permeation

Isaac¹,

Chiari²,

Miglioli³

et al. 2012

J. App. Pharm. Sci.

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“…Australian natives believe that oil of Melaleuca has antiseptic activities (Schulz, 2000). Oil of Melaleuca or Tea Tree Oil (TTO) has been reported to have anti-bacterial (Carson et al, 2006;Luz;Janaina, 2007), antifungic (Hammer et al, 1997, Oliva et al, 2003, antiviral (Bishop, 1995), anti-tumor activity (Calcabrini et al, 2004) and anti-infl ammatory properties in vitro (Brand et al, 2001;Hart et al, 2000). Oil of Melaleuca has a wide spectrum of activity that is mainly attributed to terpeno-4-ol.…”

Section: Introductionmentioning

confidence: 99%

The influence of the essential oil of Melaleuca alternifolia on the healing of infected dental alveoli: a histological study in rats

Kreuger¹,

Ternes²,

Mello³

et al. 2007

Rev. bras. farmacogn.

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RESUMO: "Infl uência do óleo essencial de Melaleuca alternifolia na cicatrização de alveolite dental infectada: um estudo histológico em ratos". A planta Melaleuca alternifolia é nativa da Austrália e a destilação de suas folhas produz um óleo essencial conhecido por óleo de Melaleuca, ou Tea tree oil, usado como antimicrobiano. Nesse estudo, foi verifi cado a atividade deste óleo no processo de reparo de alvéolos dentais infectados. 48 ratos foram utilizados (Rattus novergicus albinus, Wistar) e após a extração do dente e posterior infecção do alvéolo com Staphylococcus aureus, os animais foram separados em três grupos: Grupo I: curetagem e irrigação do alvéolo com soro fi siológico; Grupo II: curetagem e irrigação com soro fi siológico e tratamento com Rifocina 25 mg; e Grupo III: curetagem e irrigação com soro fi siológico e aplicação tópica de óleo de Melaleuca 20%. Os animais foram sacrifi cados 24 horas, 7, 14 e 21 dias após o tratamento e o processo de reparo do alvéolo dental foi analisado por microscopia ótica. Os resultados foram submetidos à análise quantitativa e qualitativa e foi possível concluir que o óleo a 20% causou um retardo no processo de reparo dos alvéolos dentais infectados dos ratos, demonstrado por maior área de necrose e menor osteogênese. Unitermos: Melaleuca alternifolia, Staphylococcus aureus, alvéolo dental. ABSTRACT:The plant Melaleuca alternifolia is native to Australia. The distillation of its leaves produces an essential oil, commonly known as oil of Melaleuca, or Tea tree oil, which present antimicrobial activity. This study investigates the action of this oil on the repair process of infected dental alveoli. 48 rats were used (Rattus novergicus albinus, Wistar). After tooth extraction and posterior infection of the dental alveoli with Staphylococcus aureus, the animals were separated into three groups: Group I: curettage and irrigation with physiologic saline solution; Group II: curettage and irrigation with physiologic saline solution and topical application of rifamycin diethylamide B 25 mg; and Group III: curettage and irrigation with physiologic saline solution and topical application of oil of Melaleuca 20%. The animals were sacrifi ced 24 hours, 7, 14 and 21 days after the treatment with powder and the repair process of the dental alveoli was analyzed using an optical microscope. The results were submitted to qualitative and quantitative analysis and it was concluded that tea tree oil at 20% caused a delay in the repair process of infected dental alveoli in rats, as demonstrated by the presence of more necrosis area and less osteogenesis.

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“…Values of inhibition halo equal to or greater than 8 mm or 10 mm are indicative of the presence of inhibitory activity (Fenner et al 2006, Lima et al 2006, Aibinu et al 2007, Dutta et al 2007, Nascimento et al 2007, Packer & Luz, 2007, Scorzoni et al 2007, Pozzati et al 2008, Menezes et al 2009, Almeida et al 2011, Cavalcanti et al 2012. These values do not predict clinical response, but are associated to products with pharmacological potential in studies of drug screening (Cavalcanti et al 2012).…”

Section: Antifungal Activitymentioning

confidence: 99%

MDGC-MS analysis of essential oils from Protium heptaphyllum (Aubl.) and their antifungal activity against Candida specie

Mobin

Lima

Almeida

et al. 2016

Rev. bras. plantas med.

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Protium heptaphyllum is found in the Amazon region, and in various Brazilian states and South American countries. Also Known as almecega, it produces an oil resin used in traditional medicine as analgesic, anti-inflammatory, cicatrizant and expectorant, it is rich in pentacyclic triterpenes and essential oil. The main objective of this study was to analyze the chemical composition of P. heptaphyllumresin (OEPh) over different extraction times and to evaluate their antifungal activity against Candida species, obtained from gardeners with onychomycosis, using the disk diffusion method. The OEPh was obtained by hydrodistillation and analyzed by Multidimensional Gas Chromatography coupled with Mass Spectrometry (MDGC / MS). Candida species were obtained from lesions on the nails of horticulturist from a community garden in the city of Teresina, Piauí, Brazil. The antifungal activity in concentrations of 1000 µg/L, 500 µg/L and 250 µg/L, PROTOCOL M44-A2 (CLSI 2009) OEPh was tested. The main constituents identified were: l-limonene, α-terpineol, p-cineol, o-cymene and α-phellandrene, however, its composition varies significantly with extraction time. All species, except C. rugosa, were inhibited with halo (≥ 14 mm) at 1000 μg / L. C. krusei is naturally resistant to the drug fluconazole, but when tested with OEPh the clinical species (case 9) demonstrated sensitivity in three dilutions (halo ≤ 10 ≥ 14) and the standard strain was inhibited at concentration of 1000 μg/Lg / L (halo 14mm). A similar situation also occurred with the standard strain of C. parapsilosis (halo ≥ 11mm). OEPh has considerable antifungal activity, which merits further investigation for alternative clinical applications, since this species is widely distributed in our community, and it presents good yields, and also has important therapeutic applications.

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Método para avaliação e pesquisa da atividade antimicrobiana de produtos de origem natural

Cited by 77 publications

References 30 publications

Development of a Topical Formulation Containing S. Lutea Extract: Stability, In Vitro Studies and Cutaneous Permeation

Development of a Topical Formulation Containing S. Lutea Extract: Stability, In Vitro Studies and Cutaneous Permeation

The influence of the essential oil of Melaleuca alternifolia on the healing of infected dental alveoli: a histological study in rats

MDGC-MS analysis of essential oils from Protium heptaphyllum (Aubl.) and their antifungal activity against Candida specie

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