Methylobacterium and Its Role in Health Care-Associated Infection

Kovaleva, Julia; Degener, John E.; Mei, Henny C. van der

doi:10.1128/jcm.03561-13

Cited by 98 publications

(72 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Many of the major bacterial groups observed in this dataset are commonly isolated from aquatic and terrestrial sources, and are therefore expected to be environmentally derived in both herbivorous and carnivorous species. Alicyclobacillus, Methylobacterium, Acinetobacter, and Agrobacterium represent some of the most abundant bacterial genera in our dataset, and all are common in soil and leaf surfaces (Vorholt, 2012; Kovaleva et al, 2014; Tianli et al, 2014). …”

Section: Discussionmentioning

confidence: 99%

Microbial Communities of Lycaenid Butterflies Do Not Correlate with Larval Diet

et al. 2016

View full text Add to dashboard Cite

Herbivores possess many counteradaptations to plant defenses, and a growing body of research describes the role of symbiotic gut bacteria in mediating herbivorous diets among insects. However, persistent bacterial symbioses have not been found in Lepidoptera, despite the fact that perhaps 99% of the species in this order are herbivorous. We surveyed bacterial communities in the guts of larvae from 31 species of lycaenid butterflies whose caterpillars had diets ranging from obligate carnivory to strict herbivory. Contrary to our expectations, we found that the bacterial communities of carnivorous and herbivorous caterpillars do not differ in richness, diversity, or composition. Many of the observed bacterial genera are commonly found in soil and plant surfaces, and we detected known homopteran endosymbionts in the guts of homopterophagous species, suggesting that larvae acquire gut bacteria from their food and environment. These results indicate that lycaenid butterflies do not rely on specific bacterial symbioses to mediate their diverse diets, and provide further evidence of taxonomically depauperate bacterial communities among Lepidoptera.

show abstract

Section: Discussionmentioning

confidence: 99%

Microbial Communities of Lycaenid Butterflies Do Not Correlate with Larval Diet

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The decontamination procedures currently employed within sterile service departments (SSDs) for the reprocessing of reusable surgical instruments are very efficient against bacterial contamination although not always as effective in tissue protein decontamination or in bacterial decontamination of endoscope channels (Lipscomb et al 2006a;Kovaleva et al 2014;Peaper et al 2015). However, highly hydrophobic proteins such as prions may remain adsorbed to surgical stainless steel (SS) surfaces (Gibbs et al 1994;Lipscomb et al 2006b;Murdoch et al 2006;Herve et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Efficacy of humidity retention bags for the reduced adsorption and improved cleaning of tissue proteins including prion-associated amyloid to surgical stainless steel surfaces

et al. 2015

View full text Add to dashboard Cite

Increasing drying time adversely affects attachment of tissue proteins and prion-associated amyloid to surgical stainless steel, and reduces the efficacy of commercial cleaning chemistries. This study tested the efficacy of commercial humidity retention bags to reduce biofouling on surgical stainless steel and to improve subsequent cleaning. Surgical stainless steel surfaces were contaminated with ME7-infected brain homogenates and left to dry for 15 to 1,440 min either in air, in dry polythene bags or within humidity retention bags. Residual contamination pre/post cleaning was analysed using Thioflavin T/SYPRO Ruby dual staining and microscope analysis. An increase in biofouling was observed with increased drying time in air or in sealed dry bags. Humidity retention bags kept both protein and prion-associated amyloid minimal across the drying times both pre- and post-cleaning. Therefore, humidity bags demonstrate a cheap, easy to implement solution to improve surgical instrument reprocessing and to potentially reduce associated hospital acquired infections.

show abstract

“…However, we found that Methylobacterium strains isolated from bathrooms were tolerant to various stresses, including surfactants like benzalkonium chloride (BAC) (8,11). Some isolates even survived in 5.0% BAC after 5.0 min of exposure, indicating that cleaners need to act with more immediacy and versatility.…”

mentioning

confidence: 99%

Pentanol and Benzyl Alcohol Attack Bacterial Surface Structures Differently

Yano

Miyahara

Morii

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

bThe genus Methylobacterium tolerates hygiene agents like benzalkonium chloride (BAC), and infection with this organism is an important public health issue. Here, we found that the combination of BAC with particular alcohols at nonlethal concentrations in terms of their solitary uses significantly reduced bacterial viability after only 5 min of exposure. Among the alcohols, Raman spectroscopic analyses showed that pentanol (pentyl alcohol [PeA]) and benzyl alcohol (BzA) accelerated the cellular accumulation of BAC. Fluorescence spectroscopic assays and morphological assays with giant vesicles indicated that PeA rarely attacked membrane structures, while BzA increased the membrane fluidity and destabilized the structures. Other fluorescent spectroscopic assays indicated that PeA and BzA inactivate bacterial membrane proteins, including an efflux pump for BAC transportation. These findings suggested that the inactivation of membrane proteins by PeA and BzA led to the cellular accumulation but that only BzA also enhanced BAC penetration by membrane fluidization at nonlethal concentrations.

show abstract

Methylobacterium and Its Role in Health Care-Associated Infection

Cited by 98 publications

References 25 publications

Microbial Communities of Lycaenid Butterflies Do Not Correlate with Larval Diet

Microbial Communities of Lycaenid Butterflies Do Not Correlate with Larval Diet

Efficacy of humidity retention bags for the reduced adsorption and improved cleaning of tissue proteins including prion-associated amyloid to surgical stainless steel surfaces

Pentanol and Benzyl Alcohol Attack Bacterial Surface Structures Differently

Contact Info

Product

Resources

About