2014
DOI: 10.18632/aging.100650
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Abstract: Methylation of N-terminal arginines of the shelterin component TRF2 is important for cellular proliferation. While TRF2 is found at telomeres, where it plays an essential role in maintaining telomere integrity, little is known about the cellular localization of methylated TRF2. Here we report that the majority of methylated TRF2 is resistant to extraction by high salt buffer and DNase I treatment, indicating that methylated TRF2 is tightly associated with the nuclear matrix. We show that methylated TRF2 drasti… Show more

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Cited by 13 publications
(13 citation statements)
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References 54 publications
(102 reference statements)
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“…The strand-specific bias toward the loss of the leading strand telomere in TRF2 ΔB cells 21 (and this study), together with other studies pointing to a particular role of TRF2 in the protection of the leading telomere, 41,42 suggests that an excessive processing of a stalled fork or a t-loop by structure specific endonucleases causes the deletion of the leading strand, while allowing the lagging strand to be fully replicated. This mechanism together with previous findings indicating that the B domain of TRF2 regulates telomere replication through arginine methylation [43][44][45] as well as the activity of WRN helicase, 46 FEN-1 flap-endonuclease 47,48 and polymerase β 47,49 predict that this TRF2 domain is particularly important for cells experiencing an elevated rate of telomere replication problems. 50 This could be particularly true in the case of supertelomerase HT1080 cells, which harbors abnormally long telomeres.…”
Section: Discussionsupporting
confidence: 72%
“…The strand-specific bias toward the loss of the leading strand telomere in TRF2 ΔB cells 21 (and this study), together with other studies pointing to a particular role of TRF2 in the protection of the leading telomere, 41,42 suggests that an excessive processing of a stalled fork or a t-loop by structure specific endonucleases causes the deletion of the leading strand, while allowing the lagging strand to be fully replicated. This mechanism together with previous findings indicating that the B domain of TRF2 regulates telomere replication through arginine methylation [43][44][45] as well as the activity of WRN helicase, 46 FEN-1 flap-endonuclease 47,48 and polymerase β 47,49 predict that this TRF2 domain is particularly important for cells experiencing an elevated rate of telomere replication problems. 50 This could be particularly true in the case of supertelomerase HT1080 cells, which harbors abnormally long telomeres.…”
Section: Discussionsupporting
confidence: 72%
“…Nonetheless, we found that high hTERT expression showed only a moderate correlation with TL (R=0.48), suggesting that the unfavorable prognosis of NB patients with high hTERT expression may be related to telomerase functions other than telomere elongation [ 6 ], such as i) transcriptional modulation of Wnt/β-catenin signaling pathway [ 45 ]; ii) enhancement of cell proliferation and/or resistance to apoptosis [ 45 ]; iii) involvement in DNA-damage repair [ 46 ]; iv) activity as RNA-dependent RNA polymerase [ 47 ]. Moreover, when telomeres become critically short, they activate a DNA damage response and trigger the induction of replicative cellular senescence that can be suppressed by over-expression of hTERT [ 3 ].…”
Section: Discussionmentioning
confidence: 99%
“…Telomeres are specific DNA regions at the ends of chromosomes that prevent DNA damage and promote genomic stability [ 1 , 2 ]. Telomeric DNA consists of tandem repeats of TTAGGG and is bound to a six subunit protein complex, referred to as shelterin or telosome, composed of TRF1, TRF2, TIN2, POT1, TPP1 and hRap1 [ 3 ]. Telomeres shorten with each round of DNA replication until a critical phase when they become dysfunctional, resulting in genomic instability [ 1 - 6 ].…”
Section: Introductionmentioning
confidence: 99%
“…Immunofluorescence was performed as described (Mitchell and Zhu, 2014;Zhu et al, 2003). FISH analysis was carried out essentially as described (McKerlie et al, 2012).…”
Section: If-fishmentioning
confidence: 99%