Methoxy polyethylene glycol modification promotes adipogenesis by inducing the production of regulatory T cells in xenogeneic acellular adipose matrix

Liu, Kaiyang; He, Yunfan; Yao, Yao; Zhang, Yuchen; Cai, Zihan; Ru, Jiangjiang; Zhang, Xiangdong; Jin, Xiaoxuan; Xu, Mimi; Li, Yibao; Ma, Qizhuan; Gao, Jianhua; Lu, Feng

doi:10.1016/j.mtbio.2021.100161

Cited by 14 publications

(17 citation statements)

References 66 publications

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“…Also, silver (Ag) NPs show proangiogenic properties and enhance the biocompatibility of the scaffolds ( Shevach et al, 2014 ; Saleh et al, 2019 ). When modified with the methoxy polyethylene glycol, an acellular adipose matrix decreases the immunogenicity and increases the adipogenicity of the scaffold ( Liu et al, 2021a ). Furthermore, polypropylene mesh coated with the ECM inhibits the M1 macrophages and increases the M1/M2 ratio ( Wolf et al, 2014 ).…”

Section: Pre-application Processingmentioning

confidence: 99%

Decellularization in Tissue Engineering and Regenerative Medicine: Evaluation, Modification, and Application Methods

Neishabouri

Khaboushan

Daghigh

et al. 2022

Front. Bioeng. Biotechnol.

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Reproduction of different tissues using scaffolds and materials is a major element in regenerative medicine. The regeneration of whole organs with decellularized extracellular matrix (dECM) has remained a goal despite the use of these materials for different purposes. Recently, decellularization techniques have been widely used in producing scaffolds that are appropriate for regenerating damaged organs and may be able to overcome the shortage of donor organs. Decellularized ECM offers several advantages over synthetic compounds, including the preserved natural microenvironment features. Different decellularization methods have been developed, each of which is appropriate for removing cells from specific tissues under certain conditions. A variety of methods have been advanced for evaluating the decellularization process in terms of cell removal efficiency, tissue ultrastructure preservation, toxicity, biocompatibility, biodegradability, and mechanical resistance in order to enhance the efficacy of decellularization methods. Modification techniques improve the characteristics of decellularized scaffolds, making them available for the regeneration of damaged tissues. Moreover, modification of scaffolds makes them appropriate options for drug delivery, disease modeling, and improving stem cells growth and proliferation. However, considering different challenges in the way of decellularization methods and application of decellularized scaffolds, this field is constantly developing and progressively moving forward. This review has outlined recent decellularization and sterilization strategies, evaluation tests for efficient decellularization, materials processing, application, and challenges and future outlooks of decellularization in regenerative medicine and tissue engineering.

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Section: Pre-application Processingmentioning

confidence: 99%

Decellularization in Tissue Engineering and Regenerative Medicine: Evaluation, Modification, and Application Methods

Neishabouri

Khaboushan

Daghigh

et al. 2022

Front. Bioeng. Biotechnol.

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“…This is the case of allo/xenograft rejection caused by the dysregulation of major histocompatibility complex (MHC), which is the most serious risk associated with transplantation ( Massaro et al, 2021 ), ( Wong and Griffiths, 2014 ). According to our previous study, immunocamouflage MHC by methoxy polyethylene glycol could effectively improve the regeneration properties of xenogeneic AAM ( Liu et al, 2021 ). On the other hand, orderly infiltration and transformation of host inflammatory cells are highly essential for adipose tissue regeneration in fat transplantation ( Cai et al, 2017a ; Cai et al, 2017b ; Cai et al, 2018 ; Liu et al, 2018 ).…”

Section: Introductionmentioning

confidence: 96%

“…On the other hand, orderly infiltration and transformation of host inflammatory cells are highly essential for adipose tissue regeneration in fat transplantation ( Cai et al, 2017a ; Cai et al, 2017b ; Cai et al, 2018 ; Liu et al, 2018 ). Appropriate infiltration of inflammatory cells (e.g., neutrophils and monocytes) at the early stage of transplantation and timely polarization of macrophages from M1 to M2 could promote adipogenic and retention rates in xenogeneic AAM ( Liu et al, 2021 ). Therefore, the reaction of host immune system to AAM can determine the regeneration outcome.…”

Section: Introductionmentioning

confidence: 99%

“…The fundamental mechanism by which an AAM maintains a dynamic balance between M1 and M2 macrophages is currently unknown ( Spiller et al, 2015 ; Swinehart and Badylak, 2016 ). The timely transformation of M1 into M2 macrophages is conducive to the adipogenesis of the graft in xenogeneic AAM transplantation model ( Liu et al, 2021 ). Optimization of the decellularization process can generate specific ECM peptides in the bio-scaffold, which leads to cell migration and integration with the host tissue.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, future studies on infiltrated macrophages in AAM should refine the classification of M2 macrophages, rather than categorizing them as M2 phenotype. The resolution phase of inflammation begins after 15 days of transplantation, while macrophage infiltration and adipogenesis are still visible 12 weeks after transplantation ( He et al, 2018 ; Lin et al, 2019 ; Liu et al, 2021 ). Therefore, it is required to further extend the terminal observation time point of the AAM graft in future studies.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Research Progress on the Immunogenicity and Regeneration of Acellular Adipose Matrix: A Mini Review

Liu

He²,

Lu³

2022

Front. Bioeng. Biotechnol.

Self Cite

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Acellular adipose matrix (AAM) has received increasing attention for soft tissue reconstruction, due to its abundant source, high long-term retention rate and in vivo adipogenic induction ability. However, the current decellularization methods inevitably affect native extracellular matrix (ECM) properties, and the residual antigens can trigger adverse immune reactions after transplantation. The behavior of host inflammatory cells mainly decides the regeneration of AAM after transplantation. In this review, recent knowledge of inflammatory cells for acellular matrix regeneration will be discussed. These advancements will inform further development of AAM products with better properties.

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Hydrophilic Components as Key Active Ingredients in Adipose‐Derived Matrix Bioscaffolds for Inducing Fat Regeneration

Ma,

Yue,

Wang

et al. 2024

Adv Healthcare Materials

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Decellularized adipose‐derived matrix (DAM) has emerged as a promising biomaterial for soft tissue reconstruction. However, due to a lack of research on its complex composition, the understanding of the key components in DAM remains limited, leading to inconsistent adipogenic properties and challenges in optimizing preparation methods purposefully. In this study, it is proposed for the first time that DAM comprises two distinct components: hydrophilic (H‐DAM) and lipophilic (L‐DAM), each with markedly different effects on fat regeneration. It is confirmed that H‐DAM is the key component for inducing fat regeneration due to its enhanced cell–cell and cell–scaffold interactions, primarily mediated by the Hedgehog signaling pathway. In contrast, L‐DAM exhibits poor cell adhesion and contains more antigenic components, leading to a higher immunoinflammatory response and reduced adipogenesis. In addition, it is found that intracellular proteins, which are more abundant in H‐DAM, can be retained as beneficial components due to their hydrophilicity, contrary to the conventional view that they shall be removed. Accordingly, a purified bioscaffold with unprecedented efficacy is proposed for fat regeneration and reduced immunogenicity. This finding provides insights for developing scaffolds for fat regeneration and promotes the realization of xenotransplantation.

show abstract

Methoxy polyethylene glycol modification promotes adipogenesis by inducing the production of regulatory T cells in xenogeneic acellular adipose matrix

Cited by 14 publications

References 66 publications

Decellularization in Tissue Engineering and Regenerative Medicine: Evaluation, Modification, and Application Methods

Decellularization in Tissue Engineering and Regenerative Medicine: Evaluation, Modification, and Application Methods

Research Progress on the Immunogenicity and Regeneration of Acellular Adipose Matrix: A Mini Review

Hydrophilic Components as Key Active Ingredients in Adipose‐Derived Matrix Bioscaffolds for Inducing Fat Regeneration

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