Methods for Rapid Detection of Foodborne Pathogens: An Overview

Mandal, Prabhat Kumar; Biswas, Ashim Kumar; Choi, Kyoung-Hee; Pal, U.K.

doi:10.3923/ajft.2011.87.102

Cited by 366 publications

(200 citation statements)

References 7 publications

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“…Biological tests with luminescent bacteria may solve this problem (Kaiser and Esterby, 1991), they often exceed well-known testing systems in rate of action, accuracy, response and relative simplicity, allow simultaneous control of significant number of toxic agents (Mandal et al, 2011;Henriques et al, 2014;Bu et al, 2013;Warawa and Lawrenz, 2014). Laboratory strains of Escherichia coli were transformed by methods of genetic engineering and molecular biology using developed recombinant plasmids with lux-genes of natural microorganisms, which allowed to obtain sensory bacterial strains with a high background level of luminescence, which showed a decrease in luminescence intensity in response to the toxic effects of environmental factors.…”

Section: Introductionmentioning

confidence: 99%

Study of Intercellular Interaction of Ruminal Microorganisms of Beef Cattle

Logachev¹,

Каримов²,

Дускаев³

et al. 2015

Asian J. of Animal Sciences

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Research objective is the assessment of toxicity level of ruminal fluid of cattle with use of indicator luminescent strains of bacteria developed on the basis of E. coli. According to the research results systemic toxic action of ruminal fluid with use of sensory bacterial strains with constitutive luminescence (E. coli K12 TG1 with clone luxCDABE genes Photobacterium leiognathi 54D10) were assessed. During the contact of native ruminal fluid with sensory luminescent bacteria E. coli dose-dependent inhibition of luminescence in the first seconds of contact was registered. However, by the second minute of contact quenching was up to 5.20±0.59% in baseline environment and decreased after two-time dilution procedure up to 102.56±4.83% with dilution 1:1024). After 30 min of incubation of mixture level of luminescence in the sample with native ruminal fluid reached the control level and over time it increased to 190.59±22.15% after 60 min of contact. Strain E. coli katG::lux, where the cloned promoter is responsible for the synthesis of catalase providing the neutralization of hydrogen peroxide demonstrated luminescence induction of 3.0 relative units at the 60th minute of contact. Study of genotoxic effects based on activation of repair proteins RecA/LexA using strain E. coli recA::lux, allowed to register luminescence inducing effects of the bacterial cells in contact with native rumen fluid.

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Section: Introductionmentioning

confidence: 99%

Study of Intercellular Interaction of Ruminal Microorganisms of Beef Cattle

Logachev¹,

Каримов²,

Дускаев³

et al. 2015

Asian J. of Animal Sciences

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“…This method is widely used not only in human disease-related clinical detection (Rodu et al, 1991;Taylor et al, 2010), but also in the evaluation of microbial population (Fierer et al, 2005;Kolb et al, 2003;Mü hling et al, 2008). PCR-based detection and quantification are also convenient, fast and reliable methods for many other biological applications (Mandal et al, 2011;van Brouwershaven et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Quantification of Alternaria brassicicola infection in the Arabidopsis thaliana and Brassica rapa subsp. pekinensis

Su’udi

Park

et al. 2013

Microbiology

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Black spot caused by Alternaria brassicicola is an important fungal disease affecting cruciferous crops, including Korean cabbage (Brassica rapa subsp. pekinensis). The interaction between Arabidopsis thaliana and Alt. brassicicola is a representative model system, and objective estimation of disease progression is indispensable for accurate functional analyses. Five strains caused black spot symptom progression on Korean cabbage and Ara. thaliana ecotype Col-0. In particular, challenge with the strains Ab44877 and Ab44414 induced severe black spot progression on Korean cabbage. Ab44877 was also highly infective on Col-0; however, the virulence of Ab44414 and the remaining strains on Col-0 was lower. To unveil the relationship between mycelial growth in the infected tissues and symptom progression, we have established a reliable quantification method using real-time PCR that employs a primer pair and dual-labelled probe specific to a unigene encoding A. brassicicola SCYTALONE DEHYDRATASE1 (AbSCD1), which is involved in fungal melanin biosynthesis. Plotting the crossing point values from the infected tissue DNA on a standard curve revealed active fungal ramification of Ab44877 in both host species. In contrast, the proliferation rate of Ab44414 in Korean cabbage was 3.8 times lower than that of Ab44877. Massive infective mycelial growth of Ab44877 was evident in Col-0; however, inoculation with Ab44414 triggered epiphytic growth rather than actual in planta ramification. Mycelial growth did not always coincide with symptom development. Our quantitative evaluation system is applicable and reliable for the objective estimation of black spot disease severity.

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“…This highlights the importance of the complementary use of cultivation-dependent and -independent detection methods for the diagnosis and prevention of food contamination and foodborne diseases. In the recent decades, there have been increasing eforts to develop and improve molecular methods for the rapid detection and characterization of pathogens in animals and animal products [105][106][107][108]. These methods, which include immunological as well as biosensorand nucleic acid-based assays (e.g., ELISA, PCR, microarrays, next generation sequencing) have an improved sensitivity and speciicity but are also time-, cost-, and labor-demanding.…”

Section: Detection Characterization and Quantiication Of Salmonella mentioning

confidence: 99%

Interaction between Salmonella and Plants: Potential Hosts and Vectors for Human Infection

Fornefeld¹,

Schierstaedt²,

Jechalke³

et al. 2017

Current Topics in Salmonella and Salmonellosis

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Fruits and vegetables are important for a healthy diet. However, when eaten raw and contaminated with human pathogens (HPs) they may cause a disease outbreak. Contamination with HPs can occur along the entire farm-to-fork production chain and Salmonella enterica is one of the most common foodborne pathogens. A range of biotic and abiotic environmental factors can inluence the complex interactions between Salmonella and plants. Moreover, the outcome of experiments largely depends on the experimental design and parameters or methods employed, and on top, on the accompanying plant microbiome and the genetic equipment of the plant and the Salmonella strain. Particularly mobile genetic elements contribute to the diversiication and adaptation of Salmonella to the plant environment. So far, litle is known about the key processes and factors inluencing the atachment and potential internalization of Salmonella in plants and the plant speciic responses. It is therefore important to beter understand the ecology of Salmonella in the soil and plant environment, in order to propose practicable recommendations for prevention of foodborne diseases. This also requires improved sensitivity and speciicity of detection methods. In this chapter, we present the current knowledge, research needs, and methodology regarding the complex interactions between Salmonella and plants.

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Methods for Rapid Detection of Foodborne Pathogens: An Overview

Cited by 366 publications

References 7 publications

Study of Intercellular Interaction of Ruminal Microorganisms of Beef Cattle

Study of Intercellular Interaction of Ruminal Microorganisms of Beef Cattle

Quantification of Alternaria brassicicola infection in the Arabidopsis thaliana and Brassica rapa subsp. pekinensis

Interaction between Salmonella and Plants: Potential Hosts and Vectors for Human Infection

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