Methods for characterization of selectivity in reversed-phase liquid chromatography

Characterization and comparison of HPLC columns for gradient elution

“…The validity of Eqs (6A) and (6B) was verified earlier in many chromatographic systems for various homologous and oligomeric series [21 23]. (The constant ml was not included as a similarity parameter, as it is generally directly proportional to the constant al [20,21,23]). (The constant ml was not included as a similarity parameter, as it is generally directly proportional to the constant al [20,21,23]).…”

Characterization and comparison of HPLC columns for gradient elution

“…The validity of Eqs (6A) and (6B) was verified earlier in many chromatographic systems for various homologous and oligomeric series [21 23]. (The constant ml was not included as a similarity parameter, as it is generally directly proportional to the constant al [20,21,23]). (The constant ml was not included as a similarity parameter, as it is generally directly proportional to the constant al [20,21,23]).…”

Molar mass effects in reversed-phase gradient polymer-elution chromatography of oligomers

“…The much larger values of al and ml obtained for PE1 than for PS can, presumably, be explained by the larger molar volume of the repeat unit for PE1 [25,26]. The much larger values of al and ml obtained for PE1 than for PS can, presumably, be explained by the larger molar volume of the repeat unit for PE1 [25,26].…”

Determination of alcohols by high-performance liquid chromatography with fluorimetric detection after pre-column derivatisation with carbazole-9-N-acetic acid and chromatographic behaviour of alcoholic derivatives

“…It is worth noting that a more general quadratic equation must usually be applied for analysis the dependence of log k9 vs. X for a wide variation of the aliphatic chain causes a non-specific increase Therefore, most subsequent derivatisation temperain retention which may be distinguished from the ture selected in experiments was 608C. Increasing the contribution of the specific terms given by the 25 reagent concentration to more than 4.5?10 mol / l interaction of the functional group of the series with (already a large excess of reagent to alcohol) did not mobile and stationary phases [31]. Here, a linear significantly alter the time and temperature needed relationship has also been observed between log k9 for derivatisation reaction to be completed.…”

Characterization and comparison of HPLC columns for gradient elution

“…The validity of Eqs (6A) and (6B) was verified earlier in many chromatographic systems for various homologous and oligomeric series [21 23]. (The constant ml was not included as a similarity parameter, as it is generally directly proportional to the constant al [20,21,23]). (The constant ml was not included as a similarity parameter, as it is generally directly proportional to the constant al [20,21,23]).…”

Molar mass effects in reversed-phase gradient polymer-elution chromatography of oligomers

“…The much larger values of al and ml obtained for PE1 than for PS can, presumably, be explained by the larger molar volume of the repeat unit for PE1 [25,26]. The much larger values of al and ml obtained for PE1 than for PS can, presumably, be explained by the larger molar volume of the repeat unit for PE1 [25,26].…”

Determination of alcohols by high-performance liquid chromatography with fluorimetric detection after pre-column derivatisation with carbazole-9-N-acetic acid and chromatographic behaviour of alcoholic derivatives

“…It is worth noting that a more general quadratic equation must usually be applied for analysis the dependence of log k9 vs. X for a wide variation of the aliphatic chain causes a non-specific increase Therefore, most subsequent derivatisation temperain retention which may be distinguished from the ture selected in experiments was 608C. Increasing the contribution of the specific terms given by the 25 reagent concentration to more than 4.5?10 mol / l interaction of the functional group of the series with (already a large excess of reagent to alcohol) did not mobile and stationary phases [31]. Here, a linear significantly alter the time and temperature needed relationship has also been observed between log k9 for derivatisation reaction to be completed.…”

Characterization and comparison of HPLC columns for gradient elution

“…The validity of Eqs (6A) and (6B) was verified earlier in many chromatographic systems for various homologous and oligomeric series [21 23]. (The constant ml was not included as a similarity parameter, as it is generally directly proportional to the constant al [20,21,23]). (The constant ml was not included as a similarity parameter, as it is generally directly proportional to the constant al [20,21,23]).…”

Molar mass effects in reversed-phase gradient polymer-elution chromatography of oligomers

“…The much larger values of al and ml obtained for PE1 than for PS can, presumably, be explained by the larger molar volume of the repeat unit for PE1 [25,26]. The much larger values of al and ml obtained for PE1 than for PS can, presumably, be explained by the larger molar volume of the repeat unit for PE1 [25,26].…”

Determination of alcohols by high-performance liquid chromatography with fluorimetric detection after pre-column derivatisation with carbazole-9-N-acetic acid and chromatographic behaviour of alcoholic derivatives

“…It is worth noting that a more general quadratic equation must usually be applied for analysis the dependence of log k9 vs. X for a wide variation of the aliphatic chain causes a non-specific increase Therefore, most subsequent derivatisation temperain retention which may be distinguished from the ture selected in experiments was 608C. Increasing the contribution of the specific terms given by the 25 reagent concentration to more than 4.5?10 mol / l interaction of the functional group of the series with (already a large excess of reagent to alcohol) did not mobile and stationary phases [31]. Here, a linear significantly alter the time and temperature needed relationship has also been observed between log k9 for derivatisation reaction to be completed.…”