Methods for Assessing Oocyte Quality: A Review of Literature

Lemseffer, Yassir; Terret, Marie-Émilie; Campillo, Clément; Labrune, Elsa

doi:10.3390/biomedicines10092184

Cited by 17 publications

(7 citation statements)

References 18 publications

(53 reference statements)

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“…Currently, the maturity of ova is primarily assessed based on the morphological features of its polar body morphology 25 26…”

Section: Methods and Analysismentioning

confidence: 99%

Efficacy and safety of recombinant human granulocyte colony-stimulating factor in patients with poor ovarian response: protocol for a multicentre, randomised, controlled trial

Zeng,

Du,

Yang

et al. 2023

BMJ Open

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IntroductionApproximately 17.5% of couples of reproductive age have experienced infertility. Women with a poor ovarian response (POR) must undergo in vitro fertilisation (IVF) and embryo transfer to achieve pregnancy. However, studies have reported a poor response to gonadotropin stimulation in women undergoing IVF-ET. Results from animal experiments suggest that granulocyte colony-stimulating factor (G-CSF) has a curative effect by protecting ovarian function, reducing follicle loss and delaying premature ovarian failure in rats caused by chemotherapy. Clinical studies on the therapeutic effect of G-CSF are deficient; therefore, this study will investigate its ability to improve ovum quality and maturity among women with POR undergoing IVF/intracytoplasmic sperm injection (ICSI).Methods and analysisThis clinical, multicentre, triple-blind, placebo-controlled trial will employ balanced randomisation (1:1). The planned sample size is 312 participants (156 subjects in each group). A total of 312 patients with infertility undergoing IVF/ICSI will be recruited from four public hospitals in China. The participants will be randomly divided into an experimental group (G-CSF) and a placebo group (control). All patients will be treated by using the progestin-primed ovarian stimulation superovulation protocol. The primary outcome will be D3 embryo quality. The number of ova obtained, MII ova and transferable embryos will be inclusive in the secondary outcome.Ethics and disseminationTo our knowledge, this is the first clinical trial to investigate the effect of G-CSF treatment timing and stimulation protocol in patients with POR in China. This study will provide new information about G-CSF efficacy among patients with POR undergoing IVF/ICSI and can help improve ovum quality and maturity. Clinical trials on this subject are deficient in China; therefore, a well-designed randomised trial is needed to determine the ability of G-CSF to improve ovum quality and maturity to facilitate conception among women with POR treated with IVF/ICSI.Trial registration numberChiCTR2200062412.

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“…Currently, the maturity of ova is primarily assessed based on the morphological features of its polar body morphology 25 26…”

Section: Methods and Analysismentioning

confidence: 99%

Efficacy and safety of recombinant human granulocyte colony-stimulating factor in patients with poor ovarian response: protocol for a multicentre, randomised, controlled trial

Zeng,

Du,

Yang

et al. 2023

BMJ Open

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“…Immature oocytes have a germinal vesicle (GV) nucleus. As they mature, the nucleus progresses through various stages, such as germinal vesicle breakdown (GVBD), metaphase I (MI), and metaphase II (MII) where it becomes fully mature [11].…”

Section: Nuclear Morphologymentioning

confidence: 99%

Unveiling maturation biomarker dynamics: A comprehensive review on In Vitro embryo production in cattle

Shilpa Doultani,

Prachi Sharma,

SP Patil

et al. 2024

World J. Adv. Res. Rev.

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In vitro embryo production (IVEP) is a revolutionary reproductive biotechnology in bovine species which enables rapid genetic gain and conservation of genetic resources. IVEP has numerous applications in field of animal breeding, conservation and research, making it a powerful tool for enhancing reproductive efficiency and genetic progress in livestock industry. The process involves manipulating gametes, including oocytes and sperm, in a laboratory setting. Success of IVEP largely relies on optimization of each step, including gamete quality, sperm preparation techniques, fertilization conditions and culture medium. Advancements in culture media formulations by additions of growth factors, antioxidants along with co-culture systems and advanced imaging techniques have improved efficiency and success rates. In this article, the authors have reviewed the importance as well as biomarkers associated with in vitro maturation for a more successful outcome of IVEP in bovines.

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“…Although very promising, assisted reproductive technologies (ART) are still inefficient in terms of overall application [ 5 ], and their outcomes are hardly predictable [ 5 , 6 ]. Although different variables need to be considered to define the success of ART, oocyte quality and selection are considered crucial, limiting embryo overproduction and shortening the time for take-home baby rates [ 5 , 7 ]. Furthermore, oocyte evaluation based on morphological criteria is still a challenge [ 7 , 8 , 9 , 10 ], although some non-invasive objective biomarkers of oocyte quality have been described [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…Although different variables need to be considered to define the success of ART, oocyte quality and selection are considered crucial, limiting embryo overproduction and shortening the time for take-home baby rates [5,7]. Furthermore, oocyte evaluation based on morphological criteria is still a challenge [7][8][9][10], although some non-invasive objective biomarkers of oocyte quality have been described [11,12].…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial Dysfunction in Advanced Maternal Aged Cumulus Cells: A Possible Link to ATP Synthase Impairment?

Almeida-Reis,

Carvalho,

Dias

et al. 2024

Biomolecules

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Age-related changes in the mitochondrial status of human cumulus cells (hCCs) impact oocyte quality; however, the relationship between hCC mitochondrial (dys)function and reproductive aging remains poorly understood. This study aimed to establish the interplay between hCC mitochondrial dysfunction and women’s reproductive potential. In this investigation, 266 women were enrolled and categorized into two groups based on their age: a young group (<35 years old) and an advanced maternal age (AMA) group (≥35 years old). Comprehensive analysis of reproductive outcomes was conducted in our population. Various mitochondrial-related parameters were analyzed across distinct subsets. Specifically, mitochondrial membrane potential (∆Ψm) and mitochondrial mass were examined in 53 samples, mtDNA content in 25 samples, protein levels in 23 samples, bioenergetic profiles using an XF24 Extracellular Flux Analyzer in 6 samples, and levels of reactive oxygen species (ROS) and adenosine triphosphate (ATP) in 39 and 43 samples, respectively. In our study, the reproductive potential of AMA women sharply decreased, as expected. Additionally, an impairment in the mitochondrial function of hCCs in older women was observed; however, no differences were found in terms of mitochondrial content. Regarding oxidative phosphorylation, metabolic profiling of hCCs from AMA women indicated a decrease in respiratory capacity, which was correlated with an age-dependent decrease in the ATP synthase (ATP5A1) protein level. However, intracellular ROS and ATP levels did not differ between groups. In conclusion, our study indicates that age-related dysfunction in hCCs is associated with impaired mitochondrial function, and, although further studies are required, ATP synthase could be relevant in this impairment.

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Methods for Assessing Oocyte Quality: A Review of Literature

Cited by 17 publications

References 18 publications

Efficacy and safety of recombinant human granulocyte colony-stimulating factor in patients with poor ovarian response: protocol for a multicentre, randomised, controlled trial

Efficacy and safety of recombinant human granulocyte colony-stimulating factor in patients with poor ovarian response: protocol for a multicentre, randomised, controlled trial

Unveiling maturation biomarker dynamics: A comprehensive review on In Vitro embryo production in cattle

Mitochondrial Dysfunction in Advanced Maternal Aged Cumulus Cells: A Possible Link to ATP Synthase Impairment?

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