Method of Determination of 9-(Alkylthio)Acridines in Urine by Capillary Liquid Chromatography

Srbek, Jan; Bosáková, Zuzana; Tesařová, Eva; Suchánková, Jana; Coufal, Pavel; Němcová, I.

doi:10.1365/s10337-004-0207-y

Cited by 2 publications

(6 citation statements)

References 19 publications

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“…When basic analytes, such as acridines or pyridoquinolines [79,80], are analyzed in reversed phase liquid chromatography triethylamine (TEA) is often added to the mobile phase. TEA interacts strongly with the free acidic silanol groups of the stationary phase [81].…”

Section: System Peaks Observed With Eluents Containing Triethylaminementioning

confidence: 99%

System peaks and their positive and negative aspects in chromatographic techniques

Srbek

Coufal²,

Bosáková

et al. 2005

J of Separation Science

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Whenever a mobile phase contains more than one component, additional signals commonly called system peaks can appear. The origin of these signals is explained through loss of equilibrium in the separation column caused by injection of analyte dissolved in a different solvent than the mobile phase. The system peaks are then generated by a relaxation process started by the non-equilibrium state. An overview of the theory and applications of the system peaks in separation methods, mainly in liquid chromatography, is presented in this paper. Only a brief theoretical discussion of the system peak origin is given as the theoretical aspects of system peak formation have already been published in many papers. The main focus of this review is to summarize applications, in which system peaks were used to measure physical or physicochemical data. Signals of system peaks are often misinterpreted but they offer valuable information about thermodynamics and kinetics of the separation process that takes place in chromatographic column.

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Section: System Peaks Observed With Eluents Containing Triethylaminementioning

confidence: 99%

System peaks and their positive and negative aspects in chromatographic techniques

Srbek

Coufal²,

Bosáková

et al. 2005

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Regarding the strongly hydrophobic nature and week basic properties of the studied 9-(alkylthio)acridines and based on the separation conditions for thioacridines pub- lished in the literature [3,[23][24][25], RP CLC realized on a LiChrosorb RP-select B capillary column was selected. The LiChrosorb RP-select B stationary phase is recommended for separation of hydrophobic alkaline compounds.…”

Section: Optimization Of Clc Separationmentioning

confidence: 99%

“…The LiChrosorb RP-select B stationary phase is recommended for separation of hydrophobic alkaline compounds. In contrast to the separation conditions published elsewhere [3,[23][24][25], the eluent was chosen to consist of methanol and water without any other eluent modifier to keep the eluent composition as simple as possible. The methanol content within the range of 100-85% v/v in the eluent for separation of the thioacridine derivatives on the LiChrosorb RP-select B capillary column was investigated.…”

Section: Optimization Of Clc Separationmentioning

confidence: 99%

“…Infrared spectroscopy, electrochemistry, and NMR were utilized to get some principal physico-chemical properties of thioacridines related to their chemical structure [14][15][16]. For separation and quantification of acridine derivatives, HPLC [17][18][19][20], CZE [21,22], capillary LC (CLC) [3,[23][24][25] as well as GC and CLC with MS [26] were investigated. The highly hydrophobic properties of acridines make possible to use RP LC.…”

Section: Introductionmentioning

confidence: 99%

“…Based on the experimental results published in the literature [3,[21][22][23][24][25], CLC and CZE were successfully employed for separation and quantification of acridine derivatives. Actually, regarding 9-(alkylthio)acridines these two techniques suffer from some disadvantages.…”

Section: Introductionmentioning

confidence: 99%

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Separation and quantification of 9‐(alkylthio)acridines by capillary micellar electrokinetic chromatography and capillary liquid chromatography

Nejmanová

Coufal²,

Bosáková

et al. 2007

J of Separation Science

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Various thioacridine derivatives are potential chemotherapeutics against various diseases which are intensively synthesized, characterized, and investigated by many research groups. Efficient, fast, and reliable separation and quantification methods for their analysis are still to be developed. MEKC and capillary LC (CLC) were applied for the separation and quantification of five highly hydrophobic, weakly basic, and structurally similar 9-(alkylthio)acridines. Since the common anionic and cationic surfactants failed to separate the strongly hydrophobic thioacridines by MEKC, sodium cholate was used in an alkaline BGE and successfully employed for their fast separation. In CLC, the weakly basic nature of the thioacridines necessitated use of LiChrosorb RP-select B sorbent as the stationary phase, which combined with a very simple mobile phase methanol/water yielded an efficient chromatographic separation system. Both, the MEKC and CLC optimized separation methods were then applied to quantify the thioacridines within a concentration range of 1.0 x 10(-5)-1.0 x 10(-3) mol/L and the obtained experimental results were critically compared. In practical terms, the MEKC analytical method can quantify the analytes much faster but with a lower reliability while the CLC method performs slower analysis with a higher repeatability of the experimental results.

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Method of Determination of 9-(Alkylthio)Acridines in Urine by Capillary Liquid Chromatography

Cited by 2 publications

References 19 publications

System peaks and their positive and negative aspects in chromatographic techniques

System peaks and their positive and negative aspects in chromatographic techniques

Separation and quantification of 9‐(alkylthio)acridines by capillary micellar electrokinetic chromatography and capillary liquid chromatography

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