Methicillin-resistant Staphylococcus aureus: epidemiology and antimicrobial susceptibility experiences from the University Hospital ‘Luigi Vanvitelli’ of Naples

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the important pathogens worldwide showing resistance to several widely used antibiotics. This has made the treatment of MRSA infections harder, especially due to their prevalence in the hospital setting. We evaluated the antibiotic susceptibility patterns of healthcare-associated MRSA infections with a focus on Vancomycin Intermediate S. Aureus (VISA) and macrolide-licosamide-streptogramin B (MLS B ) phenotypes. A total of 417 Staphylococcus aureus (S. aureus) cases were isolated between January 2017 and December 2018, through several clinical specimens collected from the University Hospital 'Luigi Vanvitelli' of Naples. We identified bacterial strains using Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) and antimicrobial susceptibility using Phoenix BD (Becton Dickinson, NJ, USA). Out of the total 417 S. aureus cases, 140 were MRSA (33.6%) and of these, 50% were soft tissue infections. All MRSA and Methicillin sensible S.aureus MSSA isolates were susceptible to linezolid and daptomycin. Two MRSA cases exhibited intermediate resistance to vancomycin and were of constitutive MLS B phenotype. Among the MRSA strains, 11.4% were constitutive and 43.6% were inducible MLS B phenotypes and 8.6% were macrolide-streptogramin B phenotype. This study characterized the epidemiological status, antibiotic resistance patterns, and current prevalent phenotypes of healthcareassociated MRSA. This knowledge can aid clinicians in improving the antimicrobial stewardship program by adapting appropriate guidelines for the proper use of MRSA antibacterial agents.

Epidemiology and antimicrobial susceptibility of Staphylococcus aureus in children in a tertiary care pediatric hospital in Milan, Italy, 2017—2021

“…We found a high proportion of MRSA (30%). The prevalence of MRSA is similar to that reported in an Italian study on the adult population (33.6%) [ 21 ] and to the one recently estimated by the Antimicrobial Resistance Collaborators in Italy in 2019 (between 30 and 40%) [ 22 ], but higher than the one reported in a Turkish pediatric study (18.2%) [ 23 ]. The stable trend in the proportion of MRSA in our pediatric sample is consistent with the stable/increasing trend in the Italian general population (from 34.1% in 2015 to 35.6% in 2019) reported by the European Centre for Disease Prevention and Control (ECDC) in the Annual Epidemiological Report for 2019 [ 24 ].…”

Sepsis—A Retrospective Cohort Study of Bloodstream Infections

“…This species showed high resistance to Oxacillin (43.7%), Erythromycin (47.8%), and Levofloxacin (38.2%). The spread of methicillin-resistant S. aureus (MRSA) is a major public health problem [36]. To date, the most suitable treatment for resolving infections caused by MRSA is represented by to use of Glycopeptides, in particular, Vancomycin.…”

Grape Canes from Typical Cultivars of Campania (Southern Italy) as a Source of High-Value Bioactive Compounds: Phenolic Profile, Antioxidant and Antimicrobial Activities

“…Each well was inoculated with 50 μL of the standardized bacterial suspension, corresponding to a final test concentration of about 5 × 10 5 CFU/mL. The antibacterial activity was expressed as the relative percentage (%) of bacterial growth after 20 h of incubation at 37 °C [ 54 , 55 ]. All experiments were performed in triplicate, and results were expressed as mean ± standard deviation (SD).…”