Methemoglobin determination by multi-component analysis in coho salmon (Oncorhynchus kisutch) possessing unstable hemoglobin

Blair, Stephanie; Barlow, Clyde H.; Martin, E. E.; Schumaker, Ruth; McIntyre, Jenifer K.

doi:10.1016/j.mex.2020.100836

Cited by 8 publications

(2 citation statements)

References 32 publications

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“…To examine hematological changes in symptomatic runofftreated coho salmon (n = 5) relative to paired controls, we measured Hct immediately after blood collection by centrifugation (12 000g for 6 min) in capillary tubes (Hesser 1960). THb, percent methemoglobin, and percent oxyhemoglobin were determined using coho spectroscopic multicomponent analysis following red cell lysis in reverse osmosis water (Blair et al 2020). MCHC was calculated by dividing THb by the Hct (Wells and Baldwin 1990).…”

Section: Hematology Experimentsmentioning

confidence: 99%

Acute cerebrovascular effects in juvenile coho salmon exposed to roadway runoff

Blair

Barlow

McIntyre

2021

Can. J. Fish. Aquat. Sci.

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Urban stormwater is responsible for recurrent mortality events of coho salmon (Oncorhynchus kisutch) documented in the US Pacific Northwest. Currently, the toxic mode of action is unknown; however, mortality is often associated with large increases in hematocrit (Hct). We found that hemoconcentration and red cell swelling may offer a partial explanation for increased Hct, based on increased total hemoglobin and reduced mean cell hemoglobin concentration of the blood in runoff-exposed juvenile coho salmon, relative to controls. No significant changes were seen in plasma total thiols or antioxidant power, discounting osmoregulatory dysfunction and plasma dehydration as a driver of hemoconcentration. To test for plasma leakage, we injected Evans blue dye complexed with bovine serum albumin (EBD–BSA) intracardially and circulated for 5 min, followed by a 15 min washout with saline. Coho salmon exposed to roadway runoff showed significant accumulation of EBD–BSA in brain and olfactory rosette regions compared with controls, demonstrating plasma leakage from the cerebrovasculature. Our results suggest blood–brain barrier disruption underlies symptom development in coho salmon exposed to roadway runoff.

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Section: Hematology Experimentsmentioning

confidence: 99%

Acute cerebrovascular effects in juvenile coho salmon exposed to roadway runoff

Blair

Barlow

McIntyre

2021

Can. J. Fish. Aquat. Sci.

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“…Furthermore, RBC senescence may be also associated with methemoglobinemia. Respiratory pigments of teleostean RBCs are more vulnerable to oxidation comparing to hemoglobin of higher vertebrates (Maestre et al, 2009;Blair et al, 2020). RBCs possess NADH-diaphorase that is responsible to maintaining hemoglobin in ferry-form (Schoore et al, 1995;Saleh, McConkey, 2012).…”

Section: Discussionmentioning

confidence: 99%

Annual variation of red blood cell number and composition of erythroid cells in circulating blood and head kidney of golden grey mullet (Chelon auratus Risso, 1810)

Солдатов

Rychkova

Кухарева

et al. 2023

Preprint

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The changes in the number of erythrocytes in the blood and the production of erythrocytes by the hematopoietic tissue of the golden grey mullet (Chelon auratus Risso, 1810) during the annual cycle were studied. Catching and delivery of fish to the aquarium was carried out monthly. The content of immature erythroid forms was determined: pronormoblasts, basophilic and polychromatophilic normoblasts, in the head kidney (pronephros) and circulating blood. It has been established that the processes of erythropoiesis in the hematopoietic tissue of the golden grey mullet proceed irregularly. The active production of erythroid mass is mainly confined to the post-spawning period. This is evidenced by an increase in the content of immature erythroid forms in the head kidney and blood. This coincides with a general increase in the number of red blood cells in the circulation system and indicates a shift in the erythrocyte balance in favor of production processes. In the rest of the time, the processes are opposite. It is assumed that this is due to the peculiarities of the organization of the red blood system, which excludes the regular production of erythropoietin in the kidneys.

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