Methanopyrus kandleri, gen. and sp. nov. represents a novel group of hyperthermophilic methanogens, growing at 110�C

Kurr, M.; Huber, Robert; König, Helmut; Jannasch, Holger W.; Fricke, Hans; Trincone, Antonio; Kristjánsson, Jakob K.; Stetter, Karl O.

doi:10.1007/bf00262992

Cited by 357 publications

(173 citation statements)

References 40 publications

Supporting

Mentioning

169

Contrasting

Unclassified

Order By: Relevance

“…Numerous thermophilic, strictly lithoautotrophic prokaryotes have been isolated from this extreme environment (Prieur et al, 1995). Most of them are hyperthermophilic archaea such as methanogens of the genera Methanopyrus (Kurr et al, 1991) and Methanococcus (Jones et al, 1983(Jones et al, , 1989Jeanthon et al, 1998Jeanthon et al, , 1999aZhao et al, 1988), hydrogenoxidizers of the genus Pyrolobus (Blo$ chl et al, 1997) and sulfur-reducers of the genus Ignicoccus (Huber et al, 2000b). In the domain Bacteria, extremely thermophilic sulfur-reducing representatives of Desulfurobacterium thermolithotrophum L'Haridon & Jeanthon, 2001) and the recently described sulfate-reducer Thermodesulfobacterium hydrogeniphilum (Jeanthon et al, 2002) complete the list of strictly thermophilic lithotrophs thriving at deep-sea vents.…”

Section: Discussionmentioning

confidence: 99%

Nautilia lithotrophica gen. nov., sp. nov., a thermophilic sulfur-reducing epsilon-proteobacterium isolated from a deep-sea hydrothermal vent.

Miroshnichenko¹,

Kostrikina²,

L’Haridon

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Nautilia lithotrophica gen. nov., sp. nov., a thermophilic sulfur-reducing ε-proteobacterium isolated from a deep-sea hydrothermal vent where it forms a deep cluster with recently isolated relatives. On the basis of phenotypic and phylogenetic differences between strain 525 T and its closest phylogenetic relatives, it is proposed that the new isolate should be described as a member of a new genus, Nautilia, for which the name Nautilia lithotrophica gen. nov., sp. nov. is proposed. The type strain is strain 525 T (l DSM 13520 T ).

show abstract

Section: Discussionmentioning

confidence: 99%

Nautilia lithotrophica gen. nov., sp. nov., a thermophilic sulfur-reducing epsilon-proteobacterium isolated from a deep-sea hydrothermal vent.

Miroshnichenko¹,

Kostrikina²,

L’Haridon

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

show abstract

“…The debate about thermophilic origins started in the wake of Karl Stetter's work, who discovered and cultured many hyperthermophiles, findings that changed the way people think about habitable environments (Fiala and Stetter 1986;Huber et al 1986;Kurr et al 1991). At the same time, John Baross was exploring the possibility that life arose at hydrothermal vents (Baross and Hoffman 1985), while Norm Pace (1991) was confronting the origin of life community with the concept of thermophilic origins, which made eminent sense to many microbiologists and which furthermore fit well with the older notion that FeS proteins held a clue to life's origins (Eck and Dayhoff 1966;Hall et al 1971), a concept that Günter Wächtershäuser (1992) developed into a more general origins theory in an early evolution context.…”

Section: Hot Debates I: Temperaturementioning

confidence: 99%

Early Microbial Evolution: The Age of Anaerobes

Martin

Sousa

2015

Cold Spring Harb Perspect Biol

View full text Add to dashboard Cite

In this article, the term "early microbial evolution" refers to the phase of biological history from the emergence of life to the diversification of the first microbial lineages. In the modern era (since we knew about archaea), three debates have emerged on the subject that deserve discussion: (1) thermophilic origins versus mesophilic origins, (2) autotrophic origins versus heterotrophic origins, and (3) how do eukaryotes figure into early evolution. Here, we revisit those debates from the standpoint of newer data. We also consider the perhaps more pressing issue that molecular phylogenies need to recover anaerobic lineages at the base of prokaryotic trees, because O 2 is a product of biological evolution; hence, the first microbes had to be anaerobes. If molecular phylogenies do not recover anaerobes basal, something is wrong. Among the anaerobes, hydrogen-dependent autotrophs-acetogens and methanogenslook like good candidates for the ancestral state of physiology in the bacteria and archaea, respectively. New trees tend to indicate that eukaryote cytosolic ribosomes branch within their archaeal homologs, not as sisters to them and, furthermore tend to root archaea within the methanogens. These are major changes in the tree of life, and open up new avenues of thought. Geochemical methane synthesis occurs as a spontaneous, abiotic exergonic reaction at hydrothermal vents. The overall similarity between that reaction and biological methanogenesis fits well with the concept of a methanogenic root for archaea and an autotrophic origin of microbial physiology.

show abstract

“…These organisms live under the most extreme conditions yet found for living organisms. Within the Archaea, some forms are active above 110 "C (Stetter et al, 1983;Kurr et al, 1991), which may be compared to the 50-70 "C temperature ranges for "classical" thermophiles such as 7: aquaticus. In addition, it is generally believed that hyperthermophiles are ancestral to organisms that grow at room temperature.…”

Section: Advantages Of the Study Of Hyperthermophilic Proteinsmentioning

confidence: 99%

The sequence of a subtilisin‐type protease (aerolysin) from the hyperthermophilic archaeum Pyrobaculum aerophilum reveals sites important to thermostability

et al. 1994

Self Cite

View full text Add to dashboard Cite

The hyperthermophilic archaeum Pyrobaculum aerophilum grows optimally at 100 "C and pH 7.0. Cell homogenates exhibit strong proteolytic activity within a temperature range of 80-130 "C. During an analysis of cDNA and genomic sequence tags, a genomic clone was recovered showing strong sequence homology to alkaline subtilisins of Bacihs sp. The total DNA sequence of the gene encoding the protease (named "aerolysin") was determined. Multiple sequence alignment with 15 different serine-type proteases showed greatest homology with subtilisins from gram-positive bacteria rather than archaeal or eukaryal serine proteases.Models of secondary and tertiary structure based on sequence alignments and the tertiary structures of subtilisin Carlsberg, BPN', thermitase, and protease K were generated for P. aerophilurn subtilisin. This allowed identification of sites potentially contributing to the thermostability of the protein. One common transition put alanines at the beginning and end of surface alpha-helices. Aspartic acids were found at the N-terminus of several surface helices, possibly increasing stability by interacting with the helix dipole. Several of the substitutions in regions expected to form surface loops were adjacent to each other in the tertiary structure model.

show abstract

Methanopyrus kandleri, gen. and sp. nov. represents a novel group of hyperthermophilic methanogens, growing at 110�C

Cited by 357 publications

References 40 publications

Nautilia lithotrophica gen. nov., sp. nov., a thermophilic sulfur-reducing epsilon-proteobacterium isolated from a deep-sea hydrothermal vent.

Nautilia lithotrophica gen. nov., sp. nov., a thermophilic sulfur-reducing epsilon-proteobacterium isolated from a deep-sea hydrothermal vent.

Early Microbial Evolution: The Age of Anaerobes

The sequence of a subtilisin‐type protease (aerolysin) from the hyperthermophilic archaeum Pyrobaculum aerophilum reveals sites important to thermostability

Contact Info

Product

Resources

About