Methanogen Levels Are Significantly Associated with Fecal Microbiota Composition and Alpha Diversity in Healthy Adults and Irritable Bowel Syndrome Patients

Wang, Taojun; Dijk, Leander van; Rijnaarts, Iris; Hermes, Gerben D. A.; Roos, N.M. de; Witteman, Ben J.M.; Wit, Nicole de; Govers, Coen; Smidt, Hauke; Zoetendal, Erwin G.

doi:10.1128/spectrum.01653-22

Cited by 6 publications

(5 citation statements)

References 52 publications

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“…This is supported by a humanized gnotobiotic mouse model colonized with Bacteroides thetaiotaomicron and M. smithii, which revealed that co-colonisation with these two microbial species led to higher population densities than when singly inoculated, and that M. smithii enhanced B. thetaiotaomicron to degrade polyfructose-containing glycans [15]. This is supported by the ndings that methanogen levels were positively associated with microbiota diversity in humans as well [20]. However, during the incubations in this study, inhibiting methane production in MEs or adding methanogens into the microbiota of NMEs did not signi cantly alter overall microbiota composition and diversity compared to the control incubations.…”

Section: Discussionmentioning

confidence: 66%

“…These so-called methane excretors (MEs) have a signi cantly higher number of methanogens in the gut with approximately 10 9 CFU/g in stool, whereas individuals who are nonmethane excretors (NMEs) harbor less than 10 4 CFU/g [17,19]. A recent study by Wang et al [20] indicated that methanogen levels were signi cantly associated with overall microbiota composition and diversity, which implies that hydrogenotrophic microbes play an important role in determining the gut microbiota composition and function. However, to what extend the presence of different hydrogen consuming microbes determine the overall microbiota composition and fermentation in MEs and NMEs remains unknown.…”

Section: Introductionmentioning

confidence: 99%

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In vitro incubation reveals the gut microbiota is resilient to changes in hydrogenotrophic activities in which methanogenesis is an optional niche

Wang

Smidt

Zoetendal

2022

Preprint

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Background: Hydrogen metabolism plays a central role in microbial fermentation. However, how hydrogenotrophic microbes impact microbiota composition and metabolite production in gut ecosystems remains largely unknown. Hence, this study investigates the impact of altering two of the key hydrogenotrophic activities, namely methanogenesis and sulphate reduction, on human gut microbiota composition and metabolite production. Fecal slurries from three methane excretors (MEs) and three non-methane excretors (NMEs) were inoculated into basal medium with pectin or a carbohydrate mixture as substrates. Methanogenesis was inhibited by adding 2-bromoethanesulfonate to ME incubations, or stimulated by adding Methanobrevibacter smithii to NME incubations. Sulphate reduction was stimulated by adding sodium sulphate to both incubations. Results: Our observations revealed that microbial richness and composition, and propionate and methane production differed significantly between MEs and NMEs. Lower hydrogen concentrations were observed in MEs compared to NMEs in the incubations with pectin, but not with the carbohydrate mixture. Remarkably, sulphate was not consumed in either ME or NME incubations. Adding M. smithii to the NME inocula resulted in its persistence in the community and methane production during incubations. The addition of 2-bromoethanesulfonate inhibited methane production in the ME incubations, accompanied with a lower relative abundance of methanogens when pectin was used as substrate. However, altering methanogenesis did not significantly change overall microbiota composition and short chain fat acid production in MEs and NMEs. Conclusions: Thesefindings suggest that methanogens can occupy a niche in a microbiota that originally lacks methanogens, but that modulating methanogenesis has a minor effect on overall microbiota composition and activity.

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Section: Discussionmentioning

confidence: 66%

Section: Introductionmentioning

confidence: 99%

In vitro incubation reveals the gut microbiota is resilient to changes in hydrogenotrophic activities in which methanogenesis is an optional niche

Wang

Smidt

Zoetendal

2022

Preprint

Self Cite

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“…Interestingly, the literature shows that SCFAs produced by microbial fermentation induce the same type of effects as increased plasma glucagon-like peptide-1 (GLP-1) and peptide YY (PYY) levels and also stimulate the secretion of gut peptides by acting on specific G-proteincoupled receptors expressed in cells abundant in the colon and terminal ileum [35][36][37][38][39][40][41].…”

Section: Introductionmentioning

confidence: 99%

The Beneficial Effects of Dietary Interventions on Gut Microbiota—An Up-to-Date Critical Review and Future Perspectives

Purdel,

Margină,

Adam-Dima

et al. 2023

Nutrients

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Different dietary interventions, especially intermittent fasting, are widely used and promoted by physicians; these regimens have been studied lately for their impact on the gut microbiota composition/function and, consequently, on the general physiopathological processes of the host. Studies are showing that dietary components modulate the microbiota, and, at the same time, the host metabolism is deeply influenced by the different products resulting from nutrient transformation in the microbiota compartment. This reciprocal relationship can potentially influence even drug metabolism for chronic drug regimens, significantly impacting human health/disease. Recently, the influence of various dietary restrictions on the gut microbiota and the differences between the effects were investigated. In this review, we explored the current knowledge of different dietary restrictions on animal and human gut microbiota and the impact of these changes on human health.

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“…Using a feedback artificial neural network designed for a certain problem and giving full play to the high-speed computing ability of the computer, the optimal solution may be found quickly. This technology can be applied in clinical medicine to detect the pathogenesis of gastroenterologyneuroscience [1][2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

From the Perspective of Computer Engineering: Research on Digestive Diseases based on Bioinformatics and Genetic Genetics

Qin¹,

Lin²,

Liu³

et al. 2023

HSET

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Background: Irritable bowel syndrome needs long-term neuroscience detection after medical engineering modeling, during which time, computer engineering is needed to complete database collection. Research methods: The research team designed this model after constructing the illusion of intervention on different sweeteners and sugars. At the same time, the artificial intelligence neural system combined with data to predict which combination of sweeteners and sugars is likely to cause disease, which combination of sugar and glycogen may lead to irritable bowel syndrome in patients, and elaborate the neural network mechanism from the perspective of calculation. Results: Through experimental data processing and analysis, the research team completed the design of the model and established a complete computer network path. Conclusion: The model can be popularized in Europe, Russia, Belarus, China, Malaysia and other regions. After cooperation with Chifeng University, supported by the Autonomous University of Barcelona in Spain and the National University of Belarus, a more in-depth comparative experiment will be carried out to find a more accurate molecular mechanism.

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Methanogen Levels Are Significantly Associated with Fecal Microbiota Composition and Alpha Diversity in Healthy Adults and Irritable Bowel Syndrome Patients

Abstract: Hydrogenotrophic microbes play an essential role in the disposal of hydrogen and the maintenance of the hydrogen balance in gut ecosystems. Their abundances vary between individuals and have been reported to be associated with human gut disorders such as irritable bowel disease.

Cited by 6 publications

References 52 publications

In vitro incubation reveals the gut microbiota is resilient to changes in hydrogenotrophic activities in which methanogenesis is an optional niche

In vitro incubation reveals the gut microbiota is resilient to changes in hydrogenotrophic activities in which methanogenesis is an optional niche

The Beneficial Effects of Dietary Interventions on Gut Microbiota—An Up-to-Date Critical Review and Future Perspectives

From the Perspective of Computer Engineering: Research on Digestive Diseases based on Bioinformatics and Genetic Genetics

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