Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer

Lapadula, Walter J.; Marcet, Paula L.; Mascotti, María Laura; Sanchez‐Puerta, M. Virginia; Ayub, Maximiliano Juri

doi:10.1038/s41598-017-01859-1

Cited by 17 publications

(26 citation statements)

References 55 publications

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“…This was the first description of the existence of RIPs in metazoans. Our research also evidenced that these genes are derived from a single HGT event from a bacterial donor species [14]. Recently, evidence has been accumulated on the role of Spiroplasma spp RIPs in the protective mechanisms generated by these endosymbiotic species against arthropod infection by natural enemies (see [15] for a review).…”

Section: Introductionmentioning

confidence: 52%

“…We have previously postulated that HGT from bacteria to mosquitoes could have been facilitated by the weakness of the Weisman barrier (the physical separation between somatic and germinal cells in multicellular organisms) at early developmental stages. Feeding of mosquitos' larva with bacterial species in their ecological niches is consistent with the acquisition of a RIP gene from prokaryotes [14].…”

Section: Btrips Are Derived From a Plant Genome Via A Single Hgt Eventmentioning

confidence: 63%

“…BLASTp and TBLASTN homology searches were performed under default parameters on metazoan databases using as query a previously reported set of RIP sequences [14]. Two automatically annotated protein sequences (GenBank: XP_018902206, XP_018902288) were retrieved from the B. tabaci MEAM 1 genome database.…”

Section: Homology Search and Sequence Analysesmentioning

confidence: 99%

“…The new B. tabaci RIP protein sequences were incorporated to our previous dataset [13]. The conserved region in the RIP domain (from Y14 to S196 residues, according to trichosanthin; number GenBank AAT91090) was selected for alignment, as it was reported [13,14,23]. MSA was constructed using MAFFT 7 online server [24] employing BLOSUM 30 as scoring matrix.…”

Section: Multiple Sequence Slignment and Phylogenetic Inferencesmentioning

confidence: 99%

“…Next, we performed phylogenetic inferences using the recently discovered BtRIPs along with a representative dataset of RIP sequences from our previous works [13,14]. The phylogenetic relationships among BtRIPs and previously characterized homologues are expected to shed light on the evolutionary origin and history of these genes and their possible relationship with homologous genes from dipterans.…”

Section: Btrip Genes Are Phylogenetically Closer To Plant Than To Mosmentioning

confidence: 99%

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Recurrent acquisition of alien ribotoxin encoding genes by insect genomes

Lapadula¹,

Mascotti

Ayub³

2019

Preprint

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Ribosome inactivating proteins (RIPs) are RNA Nglycosidases that depurinate a specific adenine residue in the conserved sarcin/ricin loop of 28S rRNA. These enzymes are widely distributed among plants and bacteria. Recently, we have described RIP genes in mosquitoes belonging to the Culicinae subfamily (Aedini and Culicini tribes).We have also shown that these genes are derived from a single event of horizontal gene transfer (HGT) from a prokaryotic donor. In the present work, we show the existence of two RIP encoding genes in the genome of the whitefly Bemisia tabaci (a hemiptera species belonging to the Aleyrodidae family, distantly related to mosquitoes). Contamination artifacts were ruled out analyzing three independent Bemisia tabaci genome databases. In contrast to mosquitoes RIPs, the whitefly genes harbor introns and, according to transcriptomic evidence, are transcribed and spliced. Interestingly, phylogenetic inference combined with taxonomic distribution strongly supports that whitefly RIP genes are derived from an independent HGT event from a plant source. Our results suggest that RIP genes fill a functional niche in insects.

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Section: Introductionmentioning

confidence: 52%

Section: Btrips Are Derived From a Plant Genome Via A Single Hgt Eventmentioning

confidence: 63%

Section: Homology Search and Sequence Analysesmentioning

confidence: 99%

Section: Multiple Sequence Slignment and Phylogenetic Inferencesmentioning

confidence: 99%

Section: Btrip Genes Are Phylogenetically Closer To Plant Than To Mosmentioning

confidence: 99%

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Recurrent acquisition of alien ribotoxin encoding genes by insect genomes

Lapadula¹,

Mascotti

Ayub³

2019

Preprint

Self Cite

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Isomorphic Fluorescent Nucleosides Facilitate Real‐Time Monitoring of RNA Depurination by Ribosome Inactivating Proteins

Cong

Ludford

et al. 2022

Chemistry A European J

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Ribosome‐inactivating proteins, a family of highly cytotoxic proteins, interfere with protein synthesis by depurinating a specific adenosine residue within the conserved α‐sarcin/ricin loop of eukaryotic ribosomal RNA. Besides being biological warfare agents, certain RIPs have been promoted as potential therapeutic tools. Monitoring their deglycosylation activity and their inhibition in real time have remained, however, elusive. Herein, we describe the enzymatic preparation and utility of consensus RIP hairpin substrates in which specific G residues, next to the depurination site, are surgically replaced with tzG and thG, fluorescent G analogs. By strategically modifying key positions with responsive fluorescent surrogate nucleotides, RIP‐mediated depurination can be monitored in real time by steady‐state fluorescence spectroscopy. Subtle differences observed in preferential depurination sites provide insight into the RNA folding as well as RIPs’ substrate recognition features.

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New ribosome-inactivating proteins and other proteins with protein synthesis–inhibiting activities

Wong

Bao

et al. 2020

Appl Microbiol Biotechnol

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Ribosome-inactivating proteins (RIPs) consist of three varieties. Type 1 RIPs are singlechained and approximately 30-kDa in molecular weight. Type 2 RIPs are double-chained and composed of a type 1 RIP chain and a lectin chain. Type III RIPs, such as maize b-32 barley and JIP60 which are produced as single-domain proenzymes, possess an N-terminal domain corresponding to the A domain of RIPs and fused to a C-terminal domain. In addition to the aforementioned three types of RIPs originating from flowering plants, there are recently discovered proteins and peptides with ribosome-inactivating and protein synthesis inhibitory activities but which are endowed with characteristics such as molecular weights distinctive from those of the regular RIPs. These new/unusual RIPs discussed in the present review encompass metazoan RIPs from Anopheles and Culex mosquitos, antimicrobial peptides derived from RIP of the pokeweed Phytolacca dioica, maize RIP (a type III RIP derived from a precursor form), RIPs from the garden pea and the kelp. In addition, RIPs with a molecular weight smaller than those of regular type 1 RIPs are produced by plants in the Cucurbitaceae family including the bitter gourd, bottle gourd, sponge gourd, ridge gourd, wax gourd, hairy gourd, pumpkin, and Chinese cucumber. A small type II RIP from camphor tree (Cinnamomum camphora) seeds and a snake gourd type II RIP with its catalytic chain cleaved into two have been reported. RIPs produced from mushrooms including the golden needle mushroom, king tuber mushroom, straw mushroom, and puffball mushroom are also discussed in addition to a type II RIP from the mushroom Polyporus umbellatus. Bacterial (Spiroplasma) RIPs associated with the fruitfly, Shiga toxin, and Streptomyces coelicolor RIP are also dealt with. The aforementioned proteins display a diversity of molecular weights, amino acid sequences, and mechanisms of action. Some of them are endowed with exploitable antipathogenic activities.

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Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer

Cited by 17 publications

References 55 publications

Recurrent acquisition of alien ribotoxin encoding genes by insect genomes

Recurrent acquisition of alien ribotoxin encoding genes by insect genomes

Isomorphic Fluorescent Nucleosides Facilitate Real‐Time Monitoring of RNA Depurination by Ribosome Inactivating Proteins

New ribosome-inactivating proteins and other proteins with protein synthesis–inhibiting activities

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