Metalloproteinases and tumor necrosis factor-alpha activities in synovial fluids of horses: 
correlation with articular cartilage alterations

Jouglin, Maggy; Robert, Céline; Valette, Jean-Paul; Gavard, Francoise; Quintin-Colonna, Françoise; Denoix, Jean‐Marie

doi:10.1051/vetres:2000136

Cited by 37 publications

(40 citation statements)

References 15 publications

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“…In LPS joints, the concentration of PGE2 in SF elevated within the first 24 h and returned to the baseline level after one week, concurring with the results of previous studies (15). Models of inflammation in horses have also demonstrated a rise in IL-1β and TNF-α concentrations (6). In addition, LPS induces IL-1β and TNF-α release, factors strongly implicated in naturally occurring arthritis (17,23).…”

Section: Discussionsupporting

confidence: 87%

Changes in Synovial Fluid Inflammatory Mediators and Cartilage Biomarkers After Experimental Acute Equine Synovitis

Wang

Jiang

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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The purpose of the study was to define transient changes in the concentration of inflammatory biomarkers and cartilage biomarkers in the synovial fluid of joints following experimentally induced acute equine synovitis. Acute synovitis was induced in eight skeletally mature mares by a sterile intra-articular injection of 1 mL of phosphate-buffered saline (PBS) containing 0.5 ng of lipopolysaccharide (LPS). The solution was injected into the right middle carpal joint. One mL of sterile PBS was injected into the left control joint. Synovial fluid was obtained at the baseline level and at 8, 24, and 168 h after injection. The levels of inflammatory biomarkers-prostaglandin E2 (PGE2), interleukin 1β (IL-1β), and tumour necrosis factor-α (TNF-α), and cartilage turnover biomarkers-collagenase-cleavage neoepitope of type II collagen (C2C) and C-terminal crosslinked telopeptide type II collagen (CTX-II) were detected with proper assays. Single injections of LPS raised the number of synovial white blood cells and concentrations of total protein, PGE2, IL-1β, TNF-α, C2C, and CTX-II. PGE2 and IL-1β rose sharply at 8 h, while TNF-α increased steadily through 8 h and 24 h, at that point; these three factors returned to the baseline level by 168 h. The time course of C2C and CTX-II concentrations peaked sharply at 24 h, and continued to be significantly elevated over the baseline level even at 168 h. Injections of LPS into the joints led to a temporal inflammatory response, which in turn increased local release of inflammatory biomarkers and significantly altered the concentrations of cartilage markers in the synovial fluid.

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Section: Discussionsupporting

confidence: 87%

Changes in Synovial Fluid Inflammatory Mediators and Cartilage Biomarkers After Experimental Acute Equine Synovitis

Wang

Jiang

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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“…Os estudos realizados para avaliar a influência das citocinas no metabolismo articular, concluíram que, principalmente, a interleucina-1 (IL-1) e o fator de necrose tumoral-alfa (TNF-a) modulam a síntese de metaloproteinases pelos condrócitos e células sinoviais, interferindo na síntese e degradação das moléculas de proteoglicanos e colágeno da matriz, e alteram o equilíbrio bioquímico necessário para a manutenção da integridade da cartilagem articular (Jouglin et al 2000, Fernandes et al 2002, Ottonello et al 2002, Schuerwegh et al 2003. Este desequilíbrio bioquímico leva a alterações degenerativas na cartilagem e, com isso, uma menor resistência às cargas compressivas impostas durante o exercício, sendo um fator predisponente para a ocorrência da osteoartrite, principalmente nos animais atletas (Chrisman et al 1981, Van de Lest et al 2000.…”

Section: Introductionunclassified

Influência do exercício na indução da apoptose e necrose das células do líquido sinovial de eqüinos atletas

Rasera

Massoco²,

Landgraf

et al. 2008

Pesq. Vet. Bras.

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Pesq. Vet. Bras. 28(5): 231-236, maio 2008 RESUMO.-Examinaram-se os efeitos do estresse mecâ-nico na resposta inflamatória e adaptativa dos tecidos articulares de cavalos atletas. O líquido sinovial foi colhido das articulações metacarpofalangeanas de eqüinos atletas antes, 3, 6 e 24 horas após o exercício, assim como de um grupo controle (cavalos não exercitados). A porcentagem de apoptose/necrose, o TNF-a e a PGE2 foram determinados pelo ensaio de AnexinaV/Iodeto de Propídeo, bioensaio (L929) e ELISA, respectivamente. Os resultados mostraram que a contagem total de células nucleadas foi sempre menor no grupo controle em relação ao grupo atleta (P<0,05). Observaram-se aumentos na porcentagem de células em apoptose (P<0,05) e necrose (P<0,05), concentração de PGE2 (P<0,05) e proteína sinovial (P<0,05), e diminuição da concentração de TNF-a (P<0,05) após 3 horas do término do exercício. O grupo atleta apresentou grau moderado de inflamação articular após o exercício intenso. Esta resposta dos tecidos articulares frente ao insulto mecânico do exercício, com maior intensidade às 3 horas após término da atividade esportiva e retornando à normalidade 24 horas após, revela a capacidade da adaptação articular ao estresse físico, em eqüinos atletas. The effects of biomechanical stress on inflammatory and adaptative responses of articular tissues in athletic horses were investigated. Synovial fluid was collected from the metacarpophalangeal joints of athletic horses before exercise and 3, 6, 24 hours after exercise, and as well as from the control group (without exercise). Apoptosis/necrosis percentage, TNF-a and PGE2 were determined by annexin V/PI assay, bioassay (L929) and ELISA, respectively. The results showed that total leukocyte count was higher in the athletic group when is compared with the control group. Three hours after the exercise was done there were increases of cellular apoptosis (P>0.05) and necrosis (P<0.05) percentage, PGE2 concentration (P<0.05) and protein concentration (P<0.05), and the TNF-a level has dropped. The athletic group showed moderate level of joint inflammation after the strenuous exercise. This articular tissue response to biomechanical insult due to the exercise, with high intensity after 3 hours after training associated with normality after 24 hours, reveals the articular adaptation to physical stress in athletic horses.

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“…In adult horses, MMP activity of synovial fluid was independent of age but was nearly twofold higher in osteoarthritis (OA) than in normal joints (Brama et al, 1998). In adult horses, a significant increase of gelatinase (MMP-9) activity was found in synovial fluids of osteoarthritic joints with severe cartilage lesions (Jouglin et al, 2000). Direct biomarkers of cartilage metabolism include markers of the anabolic process such as carboxypeptide type II (CPII), chondroitin sulphate and markers of the catabolic process such as glycosaminoglycans, keratan sulphate (KS), cartilage oligomeric matrix protein (COMP) and type II collagen fragments.…”

Section: Introductionmentioning

confidence: 99%

Plasma Concentrations of a Type II Collagen-derived Peptide and Its Nitrated Form in Growing Ardenner Sound Horses and in Horses Suffering from Juvenile Digital Degenerative Osteoarthropathy

Lejeune¹,

Serteyn

Gangl

et al. 2007

Vet Res Commun

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Several breeds of draft horses suffer from degenerative digital osteoarthropathy, resulting in a reduced active lifespan. A group of 30 Ardenner horses was followed, in standardized conditions, from 15 to 28 months of age to detect the early manifestations of the disease. The severity of the disease was assessed according to a personal grading system including clinical and radiographic items. Coll 2-1, a peptide of the helical region of type II collagen, and its nitrated form (Coll 2-1 NO 2 ) were assayed in blood plasma collected at 452 ± 18 days, 504 ± 20 days, 558 ± 18 days, 613 ± 19 days, 675 ± 19 days, 752 ± 21 days and 852 ± 19 days of age. At the end of the follow-up period, 53.3% of Ardenner horses were affected by a degenerative digital osteoarthropathy. A significant effect (p < 0.05) of time, sex and pathology was observed for Coll 2-1 NO 2 . Variations of Coll 2-1 were not significant except for the time effect. The elevation of Coll 2-1 NO 2 in the pathological group could indicate an inflammatory process during the growth of the affected horses, as nitration of tyrosine is mediated through reactive oxygen/nitrogen species and/or myeloperoxidase activity. Coll 2-1 NO 2 appears to be an interesting early marker of cartilage degradation and oxidation in degenerative osteoarthropathy.

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Metalloproteinases and tumor necrosis factor-alpha activities in synovial fluids of horses: correlation with articular cartilage alterations

Cited by 37 publications

References 15 publications

Changes in Synovial Fluid Inflammatory Mediators and Cartilage Biomarkers After Experimental Acute Equine Synovitis

Changes in Synovial Fluid Inflammatory Mediators and Cartilage Biomarkers After Experimental Acute Equine Synovitis

Influência do exercício na indução da apoptose e necrose das células do líquido sinovial de eqüinos atletas

Plasma Concentrations of a Type II Collagen-derived Peptide and Its Nitrated Form in Growing Ardenner Sound Horses and in Horses Suffering from Juvenile Digital Degenerative Osteoarthropathy

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