Metal cation saturation on montmorillonites facilitates the adsorption of DNA via cation bridging

Sheng, Xiang; Qin, Chao; Yang, Bing; Hu, Xiaojie; Liu, Cun; Waigi, Michael Gatheru; Li, Xuelin; Ling, Wanting

doi:10.1016/j.chemosphere.2019.06.159

Cited by 49 publications

(37 citation statements)

References 55 publications

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“…The observed somewhat higher sorption of substances containing several phosphate groups such as ATP, dATP and polynucleotides and no sorption of uridine which does not bear any phosphates supports this mechanism of binding. A complex of methods can be used to further explore the binding mechanism and cation bridge formation, such as specular neutron reflection (NR) and attenuated total internal reflection infrared spectroscopy (ATRIR) [52] or a combination of X-ray diffraction (XRD), inductively coupled plasma optical emission spectrometry (ICP-OES), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FTIR) [53].…”

Section: Discussionmentioning

confidence: 99%

Facile Fabrication of Natural Polyelectrolyte-Nanoclay Composites: Halloysite Nanotubes, Nucleotides and DNA Study

et al. 2020

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Complexation of biopolymers with halloysite nanotubes (HNTs) can greatly affect their applicability as materials building blocks. Here we have performed a systematic investigation of fabrication of halloysite nanotubes complexes with nucleotides and genomic DNA. The binding of DNA and various nucleotide species (polyAU, UMP Na2, ADP Na3, dATP Na, AMP, uridine, ATP Mg) by halloysite nanotubes was tested using UV-spectroscopy. The study revealed that binding of different nucleotides to the nanoclay varied but was low both in the presence and absence of MgCl2, while MgCl2 facilitated significantly the binding of longer molecules such as DNA and polyAU. Modification of the nanotubes with DNA and nucleotide species was further confirmed by measurements of ζ-potentials. DNA-Mg-modified nanotubes were characterized using transmission electron (TEM), atomic force (AFM) and hyperspectral microscopies. Thermogravimetric analysis corroborated the sorption of DNA by the nanotubes, and the presence of DNA on the nanotube surface was indicated by changes in the surface adhesion force measured by AFM. DNA bound by halloysite in the presence of MgCl2 could be partially released after addition of phosphate buffered saline. DNA binding and release from halloysite nanotubes was tested in the range of MgCl2 concentrations (10–100 mM). Even low MgCl2 concentrations significantly increased DNA sorption to halloysite, and the binding was leveled off at about 60 mM. DNA-Mg-modified halloysite nanotubes were used for obtaining a regular pattern on a glass surface by evaporation induced self-assembly process. The obtained spiral-like pattern was highly stable and resisted dissolution after water addition. Our results encompassing modification of non-toxic clay nanotubes with a natural polyanion DNA will find applications for construction of gene delivery vehicles and for halloysite self-assembly on various surfaces (such as skin or hair).

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Section: Discussionmentioning

confidence: 99%

Facile Fabrication of Natural Polyelectrolyte-Nanoclay Composites: Halloysite Nanotubes, Nucleotides and DNA Study

et al. 2020

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“…We notice that the threshold point of lake water conductivity (approximately 500 μS cm −1 ) corresponds to a pH value of 9 due to their positive linear correlation ( R = 0.65, p < 0.001, n = 219). Previous studies have shown that the negative charge of DNA and soil or sediment particles at pH ≥ 9 can highly reduce the efficiency of cations to mediate adsorption, and DNA can be desorbed almost completely from the negatively charged surface of clay minerals (Cai et al, 2006; Jia, 2020; Khanna & Stotzky, 1992; Levy‐Booth et al, 2007; Ogram et al, 1988; Romanowski et al, 1991) even if their surface cations are saturated (Sheng et al, 2019). Another possible reason worthy of consideration is competitive adsorption.…”

Section: Discussionmentioning

confidence: 99%

Preservation of sedimentary plant DNA is related to lake water chemistry

et al. 2021

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Little is currently known about preservation of plant DNA in lake sediments. Most prior information originates from laboratory experiments while systematic field‐based studies are still lacking. Here, we used the “g” and “h” universal primers for the P6 loop region of the chloroplast trnL (UAA) intron to amplify plant DNA from 219 lake surface sediments from China and Siberia. We introduce (i) the percentage of sequence counts with the best identity ≥95%, (ii) weighted average identity, (iii) weighted average DNA fragment length, and iv) rarefied richness of terrestrial seed plants of plant DNA metabarcoding as proxies for sedimentary DNA preservation and relate them to five environmental variables (lake water conductivity, lake water pH, mean July air temperature, and sampling depth, lake size) using boosted regression tree (BRT) analyses. Our results suggest that lake water chemical characteristics, that is, electrical conductivity and pH, are the most important variables for the preservation of plant DNA in lake sediments. Intermediate water conductivities (100–500 μS cm−1) and neutral to slightly alkaline water pH (7–9) may facilitate plant DNA preservation. Furthermore, deep lakes seem to support plant DNA preservation as indicated by relatively high rarefied richness. We also find high rarefied richness in small lakes compared with large lakes, but this result needs to be assessed by more studies in the future. None of our BRT models shows that mean July air temperature is a key variable to limit plant DNA preservation. To conclude, our results suggest that sedimentary DNA studies can preferentially select deep lakes characterized by intermediate water conductivities and neutral to slightly alkaline pH conditions.

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“…At very high ionic strength, electrostatic repulsion from negatively charged surfaces may be attenuated to an extent that close-contact DNA-surface attractive interactions (see below) result in DNA adsorption. The presence of divalent cations in solution may lead to increased adsorption to negatively charged sorbents via 'cation bridging' between the like-charged DNA and the sorbent [34][35][36][37] . Therefore, information on the solution ionic strength and concentrations of Ca 2+ and Mg 2+ is important to assess the extent of DNA adsorption.…”

Section: Adsorption-desorptionmentioning

confidence: 99%

The multiple states of environmental DNA and what is known about their persistence in aquatic environments

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et al. 2021

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Metal cation saturation on montmorillonites facilitates the adsorption of DNA via cation bridging

Cited by 49 publications

References 55 publications

Facile Fabrication of Natural Polyelectrolyte-Nanoclay Composites: Halloysite Nanotubes, Nucleotides and DNA Study

Facile Fabrication of Natural Polyelectrolyte-Nanoclay Composites: Halloysite Nanotubes, Nucleotides and DNA Study

Preservation of sedimentary plant DNA is related to lake water chemistry

The multiple states of environmental DNA and what is known about their persistence in aquatic environments

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