2018
DOI: 10.1038/s41598-018-26851-1
View full text |Buy / Rent full text
|
Sign up to set email alerts
|

Abstract: We present an optimised metagenomics method for detection and characterisation of all virus types including single and double stranded DNA/RNA and enveloped and non-enveloped viruses. Initial evaluation included both spiked and non-spiked bird faecal samples as well as non-spiked human faecal samples. From the non-spiked bird samples (Australian Muscovy duck and Pacific black ducks) we detected 21 viruses, and we also present a summary of a few viruses detected in human faecal samples. We then present a detail… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

6
87
0

Year Published

2018
2018
2020
2020

Publication Types

Select...
2
2

Relationship

0
4

Authors

Journals

citations
Cited by 54 publications
(96 citation statements)
references
References 66 publications
(96 reference statements)
6
87
0
Order By: Relevance
“…A powerful tool yet to be employed in the study of avian microbiomes is metagenomic sequencing (Riesenfeld et al 2004), which interrogates all gene content in the microbiome. A first use of metagenomics for identifying viruses in wild birds was recently published (Vibin et al 2018), but the bacterial microbiome has not been investigated. Metagenomic sequencing relies on shotgun sequencing and assembly of DNA fragments from the sample, and subsequently using database matching the relevant sequences to known microbial functional groups.…”
Section: Methods For Studying Gut Microbiotamentioning
confidence: 99%
“…A powerful tool yet to be employed in the study of avian microbiomes is metagenomic sequencing (Riesenfeld et al 2004), which interrogates all gene content in the microbiome. A first use of metagenomics for identifying viruses in wild birds was recently published (Vibin et al 2018), but the bacterial microbiome has not been investigated. Metagenomic sequencing relies on shotgun sequencing and assembly of DNA fragments from the sample, and subsequently using database matching the relevant sequences to known microbial functional groups.…”
Section: Methods For Studying Gut Microbiotamentioning
confidence: 99%
“…Suspected intoxication was a common history in those birds presenting en masse. Although seven birds were found dead or died prior to veterinary examination, clinical signs among the remaining birds included paresis (Figure 2a), recumbency or profound weakness (27), loss of righting reflex or difficulty righting (12), and less commonly dyspnea (4), watery or bloody diarrhea (4), clenched claws (1) and a moribund state (1). Although most birds were recumbent, they were not paralyzed as they had good cloacal tone, withdrawal reflexes, and could stand and shuffle, flap or walk across the room when stimulated.…”
Section: Resultsmentioning
confidence: 99%
“…This method relies on the unbiased sequencing of non-ribosomal RNA, and has been used to identify novel viral species in seemingly healthy animals. In Australia, for example, meta-transcriptomic approaches have been used with invasive cane toads ( Rhinella marina ) (11), waterfowl (12, 13), fish (14) and Tasmanian devils ( Sarcophilus harrisii ) (15). These approaches have also been applied diagnostically in a disease setting, including in domestic animals such as cats ( Felis sylvestris ) (16), dogs ( Canis lupus familiaris ) (17), cattle ( Bos taurus ) with respiratory diseases (1820), and pythons ( Pythonidae ) with neurological signs (21).…”
Section: Introductionmentioning
confidence: 99%
“…Monitoring studies in wild birds can be used as an early warning system for the incursion of a range of zoonotic viruses (Michel et al, 2018). Different molecular methods, including metagenomics approaches, have been developed and applied for detection and characterization of viruses in birds (Vibin et al, 2018).…”
Section: Emergence Of Viral Diseasesmentioning
confidence: 99%