Metabotropic glutamate receptors in mechanisms of plasticity of the central nervous system in the honeybee Apis mellifera

Ryzhova, I. V.; Zachepilo, T. G.; Chesnokova, E. G.; Lopatina, N. G.

doi:10.1134/s002209301003004x

Cited by 5 publications

(6 citation statements)

References 17 publications

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“…Thus, like this is observed in mammals, the preliminary activation of ImGluR1 in honeybee eliminated the inhibitory GABA action and leveled the stimulating effect of AMPA-and NMDA-receptors in the process of development of memory [21].…”

Section: Resultssupporting

confidence: 58%

Metabotropic receptor of the group I of the 5th subtype (ImGluR5) in honeybee associative olfactory learning

Lopatina

Vaido

Zachepilo

et al. 2014

J Evol Biochem Phys

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The work deals with study of character of localization in the honeybee head ganglion of metabotropic receptor ImGluR5 and its role in memory formation. With aid of pharmacological method (injections of antisense oligonucleotide and of selective receptor agonist and antagonist) and of behavioral criterion (formation and testing of preservation in memory of conditioned alimentary reflex for olfactory stimulus), there has been shown for the first time the participation of the studied receptor in formation of the honeybee long-term memory. By using the immunohistochemical method, there is first revealed the predominant expression of the ImGluRS receptor in the mushroom body Canyon cells responsible for the insect integrative activity. The present study, together with the previous ones, allows concluding about the presence in the honeybee head ganglion of the group I of metabotropic glutamate receptors with two subtypes 1 and 5 (ImGluRl,5) that have similar with mammals pharmacological properties favoring preservation of the individually acquired experience in the long-term memory.

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Section: Resultssupporting

confidence: 58%

Metabotropic receptor of the group I of the 5th subtype (ImGluR5) in honeybee associative olfactory learning

Lopatina

Vaido

Zachepilo

et al. 2014

J Evol Biochem Phys

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“…The results of Locatelli et al (2005) provided the first direct evidence for a temporally and locally restricted function of glutamate in memory formation in honeybees and insects, and more and more evidence suggested that glutamate played key roles in honeybee learning and memory process (Si et al, 2004;Kucharski et al, 2007;El Hassani et al, 2012). Besides, the data from Ryzhova et al (2010) indicated that in the mechanisms of the honeybee Central Nervous System (CNS) plasticity, an important role is played by heterogeneous of mGluRs affecting formation of the short-term and long-term memory. Furthermore, using immunohistochemistry, Démares et al (2013) found that the glutamate-gated chloride channels (GluCl) subunit variants were localized in adult honeybee mushroom bodies and antennal lobes.…”

Section: Introductionmentioning

confidence: 99%

Expression levels of glutamate and serotonin receptor genes in the brain of different behavioural phenotypes of worker honeybee (Apis mellifera)

Qin¹,

Han²,

Líu³

et al. 2014

Turkish Journal of Entomology

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Social insect colonies are known for their efficient system of task specialization. In this study, we analyzed the brain expression level of glutamate and serotonin receptor genes in different behavioural phenotypes of (Apis mellifera) workers by qRT-PCR. The glutamate receptor genes include the ionotropic glutamate receptor (iGluR) genes and metabotropic glutamate receptor (mGluRs) genes, and has 9 orthologous genes in honeybee, such as the N-methyl-D-aspartate receptor (NMDAR) genes, NMDAR1 and NMDAR2, the α-amino-3-hydroxy-5-methyl-4-isoxazole receptor (AMPAR) genes, AMPAR ∆2-a, AMPAR ∆2-b, AMPAR ∆2-c, AMPAR ∆2-d, and the mGluR1, mGluR4 and mGluR7. Our results showed that: the relative expression level of NMDAR genes was much higher in newly emerged workers (NW) than in young nurses (YN) and "old" foragers (OF) (P < 0.001); both NW and YN had a significantly higher relative expression level of AMPAR ∆2-b, AMPAR ∆2-c and AMPAR ∆2-d than in OF (P < 0.05). than in YN and OF; the relative expression level of mGluR7 gene in OF was significantly higher than in NW (P > 0.05), but there were no significant differences among NW, YN, and OF for the relative expression level of mGluR1 and mGluR4 gene (P > 0.05); in the case of Serotonin (5-HT), the relative expression level of the 5-HT1 gene showed no significant difference between YN and OF (P > 0.05), but was higher in YN and OF than in NW (P < 0.001). Above results indicate that some glutamate and serotonin receptor genes may play important roles in honeybee age-dependent role change. Key words: Apis mellifera, age, brain, iGluRs, mGluRs, 5-HT ÖzetSosyal böcekler görev dağılımının en bilinen sistemleridir. Bu çalışmada, qRT-PCR ile bal arası (Apis mellifera) işçilerinin farklı davranış fenotiplerinde beyinde bulunan glutamat ve serotonin reseptör genlerinin ekspresyon seviyeleri analiz edilmiştir. Glutamat reseptör geni; İyonotropik glutamat reseptörü (iGluR) ve metabotropik glutamat reseptörü (mGluR'ler) genlerini kapsar. Balarısında Glutamat reseptör geni, N-metil-D-aspartat reseptör (NMDAR) genleri, NMDAR1 ve NMDAR2, α-amino-3-hidroksi-5-metil-4-izoksazol reseptörü (AMPAR) genleri, AMPAR ∆2-a AMPAR ∆2-b AMPAR ∆2-c AMPAR ∆2-d ve mGluR1, mGluR4 ve mGluR7 olmak üzere 9 ortolog gene sahiptir. Sonuçlarımıza göre: NMDAR geninin ekspresyon düzeyi yeni çıkış yapmış işçilerde (NW), genç (YN) ve yaşlı bireylere (OF) oranla çok daha yüksek bulunmuşken (p<0.001); işçi arıların ve genç bireylerin her ikisinde yaşlı bireylerden belirgin bir şekilde daha yüksek oranda Ampar ∆2-b, Ampar ∆2-c ve Ampar ∆2-d gen ekspresyon düzeyi saptanmıştır (P<0.05). Yaşlı bireylerde mGluR7 geninin ekspresyon düzeyi işçi arılardan belirgin olarak daha yüksek iken (P>0.05); genç, yaşlı ve işçi arıların hepsinde mGluR1 ve mGluR4 genlerinin ekspresyon düzeyleri açısından (p>0.05) anlamlı bir fark bulunamamıştır. Serotonin (5-HT) söz konusu olduğunda, 5-HT1 geninin ekspresyon seviyesi bakımından genç ve yaşlı bireyler arasında anlamlı bir fark çıkmamıştır (P>0.05) ancak aynı genin ifadesi genç ve yaşl...

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“…Organotypic culture derived from rats of different age allowing studies of the expression of signaling molecules in different organs during aging is a convenient model for molecular and physiological studies. Insects are often used as a model for studying common biochemical and physiological mechanisms of memory trace formation in the neuroimmunoendocrine system of vertebrates and invertebrates [10,11]. The combination of these models in the same study allows tracing physiological effects of the tripeptide at the molecular, cellular, and organism level.…”

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confidence: 99%

Effect of Tripeptide Lys-Glu-Asp on Physiological Activity of Neuroimmunoendocrine System Cells

et al. 2012

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Tripeptide Lys-Glu-Asp stimulates proliferation and inhibits apoptosis in organotypic cultures of neuroimmunoendocrine system cells. Lys-Glu-Asp accelerates cell renewal processes (decrease of apoptosis marker p53 and increase of proliferation marker Ki-67) in the pineal gland; this effect is more pronounced in cultures derived from old animals than in young cultures. The tripeptide induces the expression of low-differentiated lymphocyte marker CD5 and macrophage marker CD68, but in "old" cultures this effect is less pronounced than in "young" ones. Thus, in tissue culture Lys-Glu-Asp primarily affects the nervous and endocrine tissues during aging and produces a less pronounced effect on the nervous tissue. Physiological activity of the tripeptide consists in modulation of associative learning of honey bee in the model of short-term and the long-term memory.

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Metabotropic glutamate receptors in mechanisms of plasticity of the central nervous system in the honeybee Apis mellifera

Cited by 5 publications

References 17 publications

Metabotropic receptor of the group I of the 5th subtype (ImGluR5) in honeybee associative olfactory learning

Metabotropic receptor of the group I of the 5th subtype (ImGluR5) in honeybee associative olfactory learning

Expression levels of glutamate and serotonin receptor genes in the brain of different behavioural phenotypes of worker honeybee (Apis mellifera)

Effect of Tripeptide Lys-Glu-Asp on Physiological Activity of Neuroimmunoendocrine System Cells

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