Metabolomics profile of plasma in acute diquat-poisoned patients using gas chromatography-mass spectrometry

Dai, Xinhua; Liu, Maozhu; Xu, Shuyun; Zhao, Han; Li, Xuezhi; Bai, Yangjuan; Zou, Yuangao; An, Yunfei; Fan, Fei; Zhang, Jing; Cai, Bei

doi:10.1016/j.fct.2023.113765

Cited by 5 publications

(4 citation statements)

References 49 publications

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“…Notably, DQ is a fastacting non-selective bipyridyl herbicide with a structure similar to paraquat. 7 Currently, it is widely used in agricultural production as an alternative to paraquat. The incidence of exposure to or accidental or oral ingestion of DQ is also gradually increasing, so DQ poisoning has become another problem for emergency medical personnel after paraquat poisoning.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Lac and NGAL on the Condition and Prognosis of Patients with Diquat Poisoning

Zhu,

Xu,

et al. 2023

Prehosp. Disaster med.

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Aim: This study aims to explore the evaluation of lactic acid (Lac) and neutrophil gelatinase-associated lipocalin (NGAL) on the condition and prognosis of patients with diquat (DQ) poisoning. Methods: A total of 79 cases of DQ poisoning treated in one hospital from January 2019 through February 2023 were included: 10 cases of mild poisoning, 49 cases of moderate to severe poisoning, and 20 cases of fulminant poisoning. According to the Kidney Disease: Improving Global Outcomes-acute kidney injury (KDIGO-AKI) criteria, the patients were divided into 60 cases in the AKI group and 19 cases in the non-acute kidney injury (NAKI) group. According to the AKI diagnostic indicators, AKI patients were divided into Grade I, Grade II, and Grade III. According to prognosis, the patients were divided into survivor group and non-survivor group. During the same period, 30 healthy subjects were selected as the healthy group. The changes of blood Lac, NGAL, cystatin C (CysC), and serum creatinine (Scr) levels of patients were detected, the 28-day survival of patients was recorded, and the correlation between blood Lac, NGAL levels, and renal injury grade in patients with AKI caused by DQ poisoning was analyzed. The receiver operator characteristic (ROC) curve was used to evaluate the predictive value and prognostic value of Lac, NGAL, and their combination in patients with AKI caused by DQ poisoning. Results: There were significant differences in AKI grade, Lac, NGAL, CysC, and Scr levels among different degrees of poisoning groups (P < .05). There were significant differences in the levels of Lac, NGAL, CysC, and Scr among patients with different AKI grades (P < .05). The levels of Lac, NGAL, CysC, and Scr in the survivor group were significantly lower than those in the non-survivor group (P < .05). The blood Lac and NGAL levels were positively correlated with AKI grades in patients with DQ poisoning (r = 0.752, 0.836; P = .000, .000). The combined detection of blood Lac and NGAL had higher predictive value for AKI and assessed value for death in DQ poisoning than either of them alone. Conclusion: The combined detection of Lac and NGAL have a certain clinical value in AKI grading and evaluating AKI prognosis caused by DQ poisoning.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Lac and NGAL on the Condition and Prognosis of Patients with Diquat Poisoning

Zhu,

Xu,

et al. 2023

Prehosp. Disaster med.

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“…Previously, the concentrations of amino acids were reduced to balance the osmolarity under reduced seawater salinity [44]. While phenylalanine is an essential amino acid (or it cannot be synthesized by humans), Trp is considered a conditionally indispensable amino acid because it can be synthesized by the hydroxylation of phenylalanine by the enzyme phenylalanine hydroxylase [45]. It easily induces the biosynthesis pathways (phenylalanine, tyrosine, and tryptophan biosynthesis) in adverse conditions, which involves the regulation of energy metabolism [45][46][47].…”

Section: Effects Of Low-salinity Stress On Phenylalanine Tyrosine And...mentioning

confidence: 99%

“…While phenylalanine is an essential amino acid (or it cannot be synthesized by humans), Trp is considered a conditionally indispensable amino acid because it can be synthesized by the hydroxylation of phenylalanine by the enzyme phenylalanine hydroxylase [45]. It easily induces the biosynthesis pathways (phenylalanine, tyrosine, and tryptophan biosynthesis) in adverse conditions, which involves the regulation of energy metabolism [45][46][47]. Similar results were also observed in the present study, where the expression of eight metabolites, including phenylalanine and Trp significantly decreased the regulation of amino acid metabolism under low-salinity conditions.…”

Section: Effects Of Low-salinity Stress On Phenylalanine Tyrosine And...mentioning

confidence: 99%

Effects of Low-Salinity Stress on Histology and Metabolomics in the Intestine of Fenneropenaeus chinensis

Tian,

Wang,

Gao

et al. 2024

Animals

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Metabolomics has been used extensively to identify crucial molecules and biochemical effects induced by environmental factors. To understand the effects of acute low-salinity stress on Fenneropenaeus chinensis, intestinal histological examination and untargeted metabonomic analysis of F. chinensis were performed after exposure to a salinity of 15 ppt for 3, 7, and 14 d. The histological examination revealed that acute stress resulted in most epithelial cells rupturing, leading to the dispersion of nuclei in the intestinal lumen after 14 days. Metabolomics analysis identified numerous differentially expressed metabolites (DEMs) at different time points after exposure to low-salinity stress, in which some DEMs were steadily downregulated at the early stage of stress and then gradually upregulated. We further screened 14 overlapping DEMs, in which other DEMs decreased significantly during low-salinity stress, apart from L-palmitoylcarnitine and vitamin A, with enrichments in phenylalanine, tyrosine and tryptophan biosynthesis, fatty acid and retinol metabolism, and ABC transporters. ABC transporters exhibit significant abnormalities and play a vital role in low-salinity stress. This study provides valuable insights into the molecular mechanisms underlying the responses of F. chinensis to acute salinity stress.

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“…Liquid chromatography-mass spectrometry (LC-MS), which can detect a wide range of nonvolatile and thermally unstable metabolites without derivatization, is typically utilized for metabolomics. With a metabolomics analysis, Dai et al reported that phenylalanine, tyrosine, taurine and cysteine were signi cantly different between patients treated with low and high doses of diquat 14 ; however, it was not enough to determine whether these changes led to AKI or whether clinical interventions were needed.…”

Section: Introductionmentioning

confidence: 99%

Metabolomics-based model for early warning of acute kidney injury risk in diquat-exposed patients

Zhu,

Li,

et al. 2024

Preprint

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Acute kidney injury (AKI) is one of the most important indications of severe clinical symptoms in patients with diquat poisoning and is closely related to poor prognosis. However, current studies have rarely focused on early warnings of diquat-related AKI, which is not conducive to the treatment of patients with early clinical diquat poisoning. In this study, untargeted plasma metabolomics was employed to reveal differences between diquat-poisoned patients with and without AKI, as well as between patients and healthy volunteers. The results showed that 48 metabolites were significantly changed in the patients, among which 3-hydroxybutyrylcarnitine, SAICAR, dodecanoic acid, and tetrahydrofolyl-[Glu](2) could be used to effectively differentiate the above three groups. Based on the ratios of the first two metabolites and the ratios of the last two metabolites, a decision tree model for the early warning of diquat-induced AKI was established with an accuracy and specificity of 88.7%. This model provides great support for accurate clinical diagnosis and intervention regarding the AKI risk of diquat-exposed patients.

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Metabolomics profile of plasma in acute diquat-poisoned patients using gas chromatography-mass spectrometry

Cited by 5 publications

References 49 publications

Evaluation of Lac and NGAL on the Condition and Prognosis of Patients with Diquat Poisoning

Evaluation of Lac and NGAL on the Condition and Prognosis of Patients with Diquat Poisoning

Effects of Low-Salinity Stress on Histology and Metabolomics in the Intestine of Fenneropenaeus chinensis

Metabolomics-based model for early warning of acute kidney injury risk in diquat-exposed patients

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