Metabolomics-mediated elucidation of rice responses to salt stress

Rajkumari, Nitasana; Chowrasia, Soni; Nishad, Jyoti; Ganie, Showkat Ahmad; Mondal, Tapan Kumar

doi:10.1007/s00425-023-04258-1

Cited by 6 publications

(2 citation statements)

References 193 publications

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“…The delayed metabolic changes in vpp5 were also evident in our metabolomic analysis. In rice research, metabolomic analysis has been effectively applied to explore the mechanisms behind germination and grain lling (Li et al, 2019), abiotic stress response (Rajkumari et al, 2023), and identi cation of molecular pathways associated with high grain quality (Mumm et al, 2016). In this investigation, metabolomic analysis of wildtype and vpp5 seeds supports hypothesis on the involvement of starch and sucrose metabolism in post-germinative growth of plants and demonstrated that vpp5 exhibited slower metabolic changes.…”

Section: Discussionsupporting

confidence: 56%

A vacuolar proton pump controls post-germinative growth of rice (Oryza sativa ssp. japonica)

Gann,

Nandy,

Botelho

et al. 2024

Preprint

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Post-germinative growth in plants is controlled by sucrose mobilization from the endosperm. Regulation of this process, however, is not fully understood. Transcriptional suppression of the rice vacuolar H + translocating pyrophosphatase (V-PPase) referred to as VPP5 led to enhanced starch content in mature grains. However, vpp5 suppressed lines displayed a slower post-germinative growth rescuable by sucrose, indicating inefficient sucrose mobilization from the endosperm. Biochemical and gene expression analyses showed a slower rate of starch to sucrose conversion and altered expression of the key starch conversion pathway genes. We hypothesized that the modulation of metabolic activities in the vpp5 lines was based on the perturbation in the homeostasis of H + and/or inorganic pyrophosphate (PPi). Concurring with this, we found enhanced levels of PPi in vpp5 germinating seeds. Metabolomic analysis of the germinating seeds at two different stages of germination revealed a slower rate of metabolism and a differential enrichment of metabolites associated with starch and sucrose metabolism in vpp5 compared to the wildtype. Notably, the yield traits of vpp5 lines were not impacted as grain numbers and grain weight of the greenhouse ripened seeds were similar to that of the wildtype. In conclusion, VPP5 encoding the endosperm-specific V-PPase regulates starch to sucrose conversion in germinating seeds, and its suppression results in a slower post-germinative growth but does not impact the reproductive development and grain yield of the plants.

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Section: Discussionsupporting

confidence: 56%

A vacuolar proton pump controls post-germinative growth of rice (Oryza sativa ssp. japonica)

Gann,

Nandy,

Botelho

et al. 2024

Preprint

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“…Salt stress disrupts normal cellular functions by posing osmotic and ionic challenges to plants (Lu et al, 2022;Ma et al, 2022). Salt-sensitive plant species lack efficient mechanisms to manage excessive salt, which can lead to water loss and ion toxicity (Guo et al, 2022;Rajkumari et al, 2023). In contrast, salt-tolerant plant species have evolved intricate strategies involving specialized ion transporters, osmoprotectants, and antioxidant systems (Malakar and Chattopadhyay, 2021;Arzani et al, 2023).…”

Section: Salt Sensitivity Index Of Seed Germination Related Parametersmentioning

confidence: 99%

Evaluating seed germination and early seedling growth of wild medicinal plants for saline soil cultivation

ZEBRO,

HEO

2024

Not Sci Biol

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The impact of soil salinity on various wild plant species, including Peucedanum japonicum, Astragalus membranaceus, Lepidium sativum, Acyranthes bidentata, and Platycodon grandiflorum, was investigated in this study. Different concentrations of sodium chloride (NaCl) were used to induce salt stress, and the salt sensitivity index was employed to assess species-specific responses. Among the plants studied, Lepidium sativum exhibited the lowest sensitivity to salt stress during germination across all NaCl concentrations. For instance, at 50 mM NaCl, there were decreases of 7.2% in germination percentage, 4.22% in germination energy, 0.35% in germination index, 7.2% in peak value, and 11.99% in germination value. These percentages decreased further, ranging from 15.30% to 30.92%, at 100 mM NaCl, and more substantially at 150 mM NaCl, where reductions ranged from 42.94% to 66.26%, except for the germination rate, which only decreased by 0.05%. Conversely, Peucedanum japonicum demonstrated the highest sensitivity, experiencing reductions ranging from 27.46% to 100% at 50 mM NaCl, and complete reductions (100%) at 100 and 150 mM NaCl concentrations across all evaluated parameters. In terms of seedling growth, Acyranthes bidentata displayed the lowest sensitivity, with minimal reductions observed in various parameters, while Lepidium sativum showed significant reductions in several aspects of seedling growth under salt stress. The study underscored genetic variation in response to salt stress among the evaluated plant species, suggesting Acyranthes bidentate as a promising candidate for cultivation under salt-stress conditions. This information holds significance for utilizing unfavorable lands for plant cultivation.

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Deciphering molecular regulation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) signalling networks in Oryza genus amid environmental stress

Abhijith Shankar,

Parida,

Bhardwaj

et al. 2024

Plant Cell Rep

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Metabolomics-mediated elucidation of rice responses to salt stress

Cited by 6 publications

References 193 publications

A vacuolar proton pump controls post-germinative growth of rice (Oryza sativa ssp. japonica)

A vacuolar proton pump controls post-germinative growth of rice (Oryza sativa ssp. japonica)

Evaluating seed germination and early seedling growth of wild medicinal plants for saline soil cultivation

Deciphering molecular regulation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) signalling networks in Oryza genus amid environmental stress

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