Metabolomics for characterization of gender differences in patients infected with dengue virus

Shahfiza, Nurul; Osman, Hasnah; Hock, Tang Thean; Shaari, Khozirah; Abdel-Hamid, Abdel-Hamid Z.

doi:10.1016/j.apjtm.2015.05.012

Cited by 15 publications

(13 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, altered lipid metabolism is not unique to CMV infection. Hepatitis C virus (HCV) , human immunodeficiency virus (HIV) , and dengue virus are also associated with dyslipidemia.…”

Section: Introductionmentioning

confidence: 99%

The association between cytomegalovirus infection, obesity, and metabolic syndrome in U.S. adult females

Fleck-Derderian

McClellan

Wojcicki

2017

Obesity

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

The association between cytomegalovirus infection, obesity, and metabolic syndrome in U.S. adult females

Fleck-Derderian

McClellan

Wojcicki

2017

Obesity

View full text Add to dashboard Cite

show abstract

“…Different responses to the four serotypes were observed, which could be related to the broad range of manifestations and different DENV infection outcomes observed in the clinical setting. In addition, it was recently shown that gender discriminates the urine metabolic profile in subjects with DENV infection, and, like the in vitro study, changes in the levels of TCA cycle intermediates and amino acids contributed to the discrimination of groups according to infection outcomes (19).…”

mentioning

confidence: 99%

¹ H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patients

El‐Bacha

Struchiner

Cordeiro

et al. 2016

J Virol

View full text Add to dashboard Cite

Dengue, due to its global burden, is the most important arthropod-borne flavivirus disease, and early detection lowers fatality rates to below 1%. Since the metabolic resources crucial for viral replication are provided by host cells, detection of changes in the metabolic profile associated with disease pathogenesis could help with the identification of markers of prognostic and diagnostic importance. We applied 1 H nuclear magnetic resonance exploratory metabolomics to study longitudinal changes in plasma metabolites in a cohort in Recife, Brazil. To gain statistical power, we used innovative paired multivariate analyses to discriminate individuals with primary and secondary infection presenting as dengue fever (DF; mild) and dengue hemorrhagic fever (DHF; severe) and subjects with a nonspecific nondengue (ND) illness (ND subjects). Our results showed that a decrease in plasma low-density lipoprotein (LDL) and very-low-density lipoprotein (VLDL) discriminated dengue virus (DENV)-infected subjects from ND subjects, and also, subjects with severe infection even presented a decrease in lipoprotein concentrations compared to the concentrations in subjects with mild infection. These results add to the ongoing discussion that the manipulation of lipid metabolism is crucial for DENV replication and infection. In addition, a decrease in plasma glutamine content was characteristic of DENV infection and disease severity, and an increase in plasma acetate levels discriminated subjects with DF and DHF from ND subjects. Several other metabolites shown to be altered in DENV infection and the implications of these alterations are discussed. We hypothesize that these changes in the plasma metabolome are suggestive of liver dysfunction, could provide insights into the underlying molecular mechanisms of dengue virus pathogenesis, and could help to discriminate individuals at risk of the development of severe infection and predict disease outcome. IMPORTANCEDengue, due to its global burden, is the most important mosquito-borne viral disease. There is no specific treatment for dengue disease, and early detection lowers fatality rates to below 1%. In this study, we observed the effects of dengue virus infection on the profile of small molecules in the blood of patients with mild and severe infection. Variations in the profiles of these small molecules reflected the replication of dengue virus in different tissues and the extent of tissue damage during infection. The results of this study showed that the molecules that changed the most were VLDL, LDL, and amino acids. We propose that these changes reflect liver dysfunction and also that they can be used to discriminate subjects with mild dengue from those with severe dengue. D engue, due to its global burden, is the most important arthropod-borne disease nowadays. Dengue is caused by dengue virus (DENV), a positive-strand RNA virus with four distinct serotypes: DENV serotype 1 (DENV1) to DENV4. Estimates of the DENV infection burden at global levels indicate approximately 40...

show abstract

“…As described in our previous article (Shahfiza et al, 2015), 1 H NMR spectra of the urine samples from both infected and healthy individuals were acquired with AVANCE III 500 MHz Bruker spectrometer with BBO broadband probe using TSP (δ 0.00 ppm) as an internal standard and D 2 O as the frequency lock at 300 K.…”

Section: Equipmentmentioning

confidence: 99%

“…Biological samples. Sample size was calculated using Sample Size Program (http//www.kck.usm.my/ppsg/ stats_resources.htm), while in clusion an dexclusion criteria were considered as described by Shahfiza et al, (2015).…”

Section: Equipmentmentioning

confidence: 99%

See 1 more Smart Citation

Metabolomics approach for multibiomarkers determination to investigate dengue virus infection in human patients

Shahfiza¹,

Osman²,

Hock³

et al. 2017

Acta Biochim Pol

Self Cite

View full text Add to dashboard Cite

show abstract

Metabolomics for characterization of gender differences in patients infected with dengue virus

Abstract: The (1)H NMR metabolomic investigation combined with appropriate algorithms and pattern recognition procedures, gave an evidence for the existence of distinct metabolic differentiation of individuals, according to their gender, modulates with the infection risk.

Cited by 15 publications

References 23 publications

The association between cytomegalovirus infection, obesity, and metabolic syndrome in U.S. adult females

The association between cytomegalovirus infection, obesity, and metabolic syndrome in U.S. adult females

¹ H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patients

Metabolomics approach for multibiomarkers determination to investigate dengue virus infection in human patients

Contact Info

Product

Resources

About

Metabolomics for characterization of gender differences in patients infected with dengue virus

Abstract: The (1)H NMR metabolomic investigation combined with appropriate algorithms and pattern recognition procedures, gave an evidence for the existence of distinct metabolic differentiation of individuals, according to their gender, modulates with the infection risk.

Cited by 15 publications

References 23 publications

The association between cytomegalovirus infection, obesity, and metabolic syndrome in U.S. adult females

The association between cytomegalovirus infection, obesity, and metabolic syndrome in U.S. adult females

1 H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patients

Metabolomics approach for multibiomarkers determination to investigate dengue virus infection in human patients

Contact Info

Product

Resources

About

¹ H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patients