Metabolomics-based component profiling of Halomonas sp. KM-1 during different growth phases in poly(3-hydroxybutyrate) production

Abstract

To investigate the relationship between the production of poly(3-hydroxybutyrate) (PHB) and metabolic changes during different growth phases, a non-sterile batch fermentation process involving an alkalophilic and halophilic bacterium, Halomonas sp. KM-1, was used. Intracellular metabolites were analyzed using gas chromatography-mass spectrometry to characterize the metabolic profile. Significant changes relating to PHB production were observed in the TCA cycle, lipid-synthesis and amino acid biosynthetic pathways were found to shift dramatically between the exponential growth and stationary phases. During the stationary phase, 17 metabolites were upregulated and a cell dry mass of 17.8 g/L that included 44.8% PHB was observed at 24h in 5% glucose-supplemented cultures, whereas 11 metabolites were upregulated and a cell dry mass of 38.4 g/L that included 73.7% PHB was observed at 36 h in 10% glucose-supplemented cultures. This study provides pattern analysis of metabolite regulation during PHB accumulation, indicating that multicomponent and phase-specific mechanisms are involved.

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“…workers reported that along with PHA production other products like glycerol and different amino acids viz., serine, glycine and glutamine might also be generated when glucose is used as a substrate (Jin et al, 2013). Hence the unknown organic matter (0.37 g/l) may contribute to byproducts such as glycerol and amino acids.…”

Metabolic Pathway Profiling in Intracellular and Extracellular Environments of Streptococcus thermophilus During pH-Controlled Batch Fermentations

“…The metabolic profiling of Halomonas sp. during different growth phases characterized the metabolites regulation pattern, which is beneficial to optimize the production of poly(3-hydroxybutyrate) (Jin et al, 2013). Similar study of Saccharomyces cerevisiae during different growth stages revealed that the oscillation of metabolites abundance contributed to controlling the temporal regulation of cellular processes (Tu et al, 2007).…”