Metabolomic study of myocardial ischemia and intervention effects of Compound Danshen Tablets in rats using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry

Lv, Yonghai; Liu, Xinru; Yan, Shaomin; Liang, Xin; Yang, Yi; Dai, Weixing; Zhang, Wei‐Dong

doi:10.1016/j.jpba.2009.12.016

Cited by 68 publications

(40 citation statements)

References 32 publications

(31 reference statements)

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“…The supernatant was transferred into a new tube and completely dried under a gentle stream of N 2 . Prior to HPLC-QTOF-MS analysis, the dried samples were reconstituted with 100 µL of 80 % acetonitrile prepared in deionized water [25].…”

Section: Sample Preparationmentioning

confidence: 99%

Investigation of vitamin B6 inadequacy, induced by exposure to the anti-B6 factor 1-amino d-proline, on plasma lipophilic metabolites of rats: a metabolomics approach

2015

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Section: Sample Preparationmentioning

confidence: 99%

Investigation of vitamin B6 inadequacy, induced by exposure to the anti-B6 factor 1-amino d-proline, on plasma lipophilic metabolites of rats: a metabolomics approach

2015

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“…Metabolome studies were already performed using serum, plasma or urine samples (Illig et al 2010;Lv et al 2010;Seeger 2009;Zhao et al 2010). However, the use of dried blood has been shown to be advantageous for large scale studies due to the easy sample management, storage and shipment (Leichtle et al 2010;Michopoulos et al 2010).…”

Section: Specimen Collection Conditionsmentioning

confidence: 99%

Preanalytical standardization of amino acid and acylcarnitine metabolite profiling in human blood using tandem mass spectrometry

et al. 2010

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Quantitative metabolite profiling in biological samples has the potential to reflect physiological status and to identify disease associated disturbances in metabolic networks. However, this approach is hampered by a wide range of preanalytical variables. Hence, the aim of our study was to develop a standardized preanalytical protocol for metabolite profiling of amino acids and acylcarnitines in human blood. Amino acids and acylcarnitines were simultaneous analyzed after butylation of 3 lL dried blood or 10 lL whole blood, serum and anticoagulated plasma using electrospray tandem-mass spectrometry. The influence of exogenous and endogenous preanalytical variables was investigated in healthy volunteers. Different sampling materials and anticoagulants for blood taking were investigated. Concentrations of long-chain acylcarnitines were 5-fold higher in EDTA-whole blood or dried whole blood compared to serum and anticoagulated plasma. Significant differences in amino acid concentrations were found for capillary versus venous blood taking. Fasting for 8 h before specimen collection minimized the nutritional influence. Physical activity significantly alters amino acid and short chain acylcarnitine concentrations. As a result of our preanalytical investigation we developed a pre-treatment protocol based on EDTA whole blood dried on filter paper to reduce the preanalytical variability and facilitate reproducible quantitative metabolite profiling in clinical trials.

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“…It is a new tool to pinpoint changed metabolites in the body in response to a disease process or drug therapy (Constantinou et al, 2007;Julian, 2004;Nebert and Vesell, 2006;Yang et al, 2004). In our previous metabolomic study on intervention effects of CDF on myocardial ischemia (MI) model (Lv et al, 2010b), 22 biomarkers of MI were identified. Meanwhile, it was proved that CDF showed a well reverse effect on the MI model.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic strategy to study therapeutic and synergistic effects of tanshinone IIA, salvianolic acid B and ginsenoside Rb1 in myocardial ischemia rats

Liu

Liang

et al. 2011

Journal of Ethnopharmacology

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Metabolomic study of myocardial ischemia and intervention effects of Compound Danshen Tablets in rats using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry

Cited by 68 publications

References 32 publications

Investigation of vitamin B6 inadequacy, induced by exposure to the anti-B6 factor 1-amino d-proline, on plasma lipophilic metabolites of rats: a metabolomics approach

Investigation of vitamin B6 inadequacy, induced by exposure to the anti-B6 factor 1-amino d-proline, on plasma lipophilic metabolites of rats: a metabolomics approach

Preanalytical standardization of amino acid and acylcarnitine metabolite profiling in human blood using tandem mass spectrometry

Metabolomic strategy to study therapeutic and synergistic effects of tanshinone IIA, salvianolic acid B and ginsenoside Rb1 in myocardial ischemia rats

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