Metabolomic studies of amino acid analysis in Saccharomyces cells exposed to selenium and gamma irradiation

Batista, Josimar Marques; Neves, Maria J.; Pereira, Alline Gomes; Gonçalves, Letícia Satler; Menezes, Helvécio Costa; Cardeal, Zenilda de Lourdes

doi:10.1016/j.ab.2020.113666

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…Several studies have indicated changes in amino acid levels in tissues, , blood, − urine, , and nonmammalian systems , following radiation exposure. Generation of ROS can lead to protein oxidation , that has led to the investigation of amino acid mixtures as IR mitigators .…”

Section: Resultsmentioning

confidence: 99%

Impact of Partial Body Shielding from Very High Dose Rates on Untargeted Metabolomics in Biodosimetry

Pannkuk,

Laiakis,

Garty

et al. 2024

ACS Omega

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A realistic exposure to ionizing radiation (IR) from an improvised nuclear device will likely include individuals who are partially shielded from the initial blast delivered at a very high dose rate (VHDR). As different tissues have varying levels of radiosensitivity, e.g., hematopoietic vs gastrointestinal tissues, the effects of shielding on radiation biomarkers need to be addressed. Here, we explore how biofluid (urine and serum) metabolite signatures from male and female C57BL/6 mice exposed to VHDR (5−10 Gy/s) total body irradiation (TBI, 0, 4, and 8 Gy) compare to individuals exposed to partial body irradiation (PBI) (lower body irradiated [LBI] or upper body irradiated [UBI] at an 8 Gy dose) using a data-independent acquisition untargeted metabolomics approach. Although sex differences were observed in the spatial groupings of urine signatures from TBI and PBI mice, a metabolite signature (N6,N6,N6-trimethyllysine, carnitine, propionylcarnitine, hexosamine-valine-isoleucine, taurine, and creatine) previously developed from variable dose rate experiments was able to identify individuals with high sensitivity and specificity, irrespective of radiation shielding. A panel of serum metabolites composed from previous untargeted studies on nonhuman primates had excellent performance for separating irradiated cohorts; however, a multiomic approach to complement the metabolome could increase dose estimation confidence intervals. Overall, these results support the inclusion of small-molecule markers in biodosimetry assays without substantial interference from the upper or lower body shielding.

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Section: Resultsmentioning

confidence: 99%

Impact of Partial Body Shielding from Very High Dose Rates on Untargeted Metabolomics in Biodosimetry

Pannkuk,

Laiakis,

Garty

et al. 2024

ACS Omega

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“…Amino acids, as a group of polar chemical compounds, should also be modified by using a derivatization reagent to make them volatile and thus accessible for GC-MS [ 27 ]. Over the past two decades, many researchers had described the ultrahigh-performance liquid chromatography-tandem mass spectrometry- (UHPLC-MS/MS-) based method for a targeted metabolomic study with increasing levels of sophistication [ 28 – 30 ]. Out of these considerations, thirty-four endogenous amino acids in human biological samples were involved in our study by using the LC-MS technique.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of 34 Amino Acids in Tumor Tissues from Colorectal Cancer Patients Based on the Targeted UHPLC-MS/MS Method

Yang

Zhang

Gao

et al. 2020

Journal of Analytical Methods in Chemistry

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A targeted ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was established and validated for the simultaneous determination of 34 amino acids in tissue samples from colorectal cancer (CRC) patients. The chromatographic separation was achieved on an Agilent ZORBAX SB-C18 column (3.0 × 150 mm, 5 μm) with a binary gradient elution system (A, 0.02% heptafluorobutyric acid and 0.2% formic acid in water, v/v; B, methanol). The run time was 10 min. The multiple reaction monitoring mode was chosen with an electrospray ionization source operating in the positive ionization mode for data acquisition. The linear correlation coefficients were >0.99 for all the analytes in their corresponding calibration ranges. The sample was pretreated based on tissue homogenate and protein precipitation with a 100 mg aliquot sample. The average recovery and matrix effect for 34 amino acids and 3 internal standards were 39.00%∼146.95% and 49.45%∼173.63%, respectively. The intra- and interday accuracy for all the analytes ranged from −13.52% to 14.21% (RSD ≤8.57%) and from −14.52% to 12.59% (RSD ≤10.31%), respectively. Deviations of stability under different conditions were within ±15% for all the analytes. This method was applied to simultaneous quantification of 34 amino acids in tissue samples from 94 CRC patients.

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A green and fast microwave-assisted synthesis of selenium nanoparticles and their characterization under gastrointestinal conditions using mass spectrometry

et al. 2023

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Metabolomic studies of amino acid analysis in Saccharomyces cells exposed to selenium and gamma irradiation

Cited by 4 publications

References 25 publications

Impact of Partial Body Shielding from Very High Dose Rates on Untargeted Metabolomics in Biodosimetry

Impact of Partial Body Shielding from Very High Dose Rates on Untargeted Metabolomics in Biodosimetry

Simultaneous Determination of 34 Amino Acids in Tumor Tissues from Colorectal Cancer Patients Based on the Targeted UHPLC-MS/MS Method

A green and fast microwave-assisted synthesis of selenium nanoparticles and their characterization under gastrointestinal conditions using mass spectrometry

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