Metabolomic strategy to study therapeutic and synergistic effects of tanshinone IIA, salvianolic acid B and ginsenoside Rb1 in myocardial ischemia rats

Lu, Yonghai; Liu, Xinru; Liang, Xin; Li, Xiang; Zhang, Wei‐Dong

doi:10.1016/j.jep.2010.11.048

Cited by 34 publications

(25 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Treatment of DM with Traditional Chinese Medicine (TCM) has a very long time. Danshen is widely used in Chinese medicine, including Compound Danshen Dripping Pill, Compound Danshen Tablet, and Compound Danshen Granule, for the treatment of cardiovascular diseases (Lu et al 2011). Sal B is a typical bioactive compound isolated from Danshen.…”

Section: Discussionmentioning

confidence: 99%

Protection of SAL B with H9C2 cells

Sun

Zuo

et al. 2015

Pharmaceutical Biology

View full text Add to dashboard Cite

Context: Salvianolic acid B (Sal B) is regarded as a potent antidiabetic agent and has been reported to possess cardioprotective effect in vivo. Objective: This study investigated the cardioprotective effects of Sal B on H9c2 cells injury caused by high glucose in vitro, and clarified the possible mechanisms. Materials and methods: Di ferent concentrations of Sal B were incubated with cells for 12 h prior being exposed to high glucose for 24 h. Cardioprotective effects of Sal B were evaluated using CCK-8 assay, ELISA, Hoechst 33258 nucleus staining, and western blot. Results: Following a 24 h exposure of H9c2 to high glucose, obvious reduction was found in cell viability (45%), GSH (54.8 ± 9.4 ng/mg protein), catalase (1.22 ± 0.12 U/mg protein), and GPX level (67.9 ± 9.4 U/mg protein), which were associated with the increases of GSSG (1.99 ± 0.28 ng/mg protein) and ROS (2.00 ± 0.19 RFU/mg protein) production. High glucose also elevated IL-6 (1.8-fold), IL-1b (1.9-fold), and TNF-a (1.6-fold) level, as well as induced cell apoptosis and NF-kB (6.1-fold) activation. However, Sal B (25 and 50 mM) elevated cell viability (28% and 44%), ameliorated oxidative stress (GSH, 1.3-and 1.6-fold; catalase, 1.9-and 2.0-fold; GPX, 1.1-and 1.4-fold; GSSG, 0.9-and 0.8-fold; ROS, 0.6-and 0.5-fold), and inflammatory response (IL-6, 0.9-and 0.7-fold; IL-1b, 0.8-and 0.6-fold; TNF-a, 0.9-and 0.8-fold), and inhibited cell apoptosis and NF-kB (0.5-and 0.2-fold) expression. Conclusion: Sal B attenuated high glucose-induced injury and cytotoxicity through inhibiting inflammatory cytokine production in H9c2 cardiac cells. ARTICLE HISTORY

show abstract

Section: Discussionmentioning

confidence: 99%

Protection of SAL B with H9C2 cells

Sun

Zuo

et al. 2015

Pharmaceutical Biology

View full text Add to dashboard Cite

show abstract

“…The shifts in the urinary TCA cycle, as well as the pentose phosphate pathway, suggested that SLF may diminish the cardiac injury of myocardial infarction through its potential pharmacological effect in the regulation of myocardial energy metabolism. Using UPLC-MS chinmedomics, coupled with partial least squares discriminate analysis, Lu and associates studied the therapeutic and synergistic effects of TSG (a combination of tanshinone IIA, salvianolic acid B, and ginsenoside Rb1), which are the three major active ingredients of Compound Danshen Formulae (CDF), for its protective effects on myocardial ischemia (Lu et al, 2011). The results suggested that chinmedomics offers a new idea for Chinese medicine research.…”

Section: Analysis Of Chinmediformulae Using Chinmedomicsmentioning

confidence: 99%

Future Perspectives of Chinese Medical Formulae: Chinmedomics as an Effector

Wang

Zhang

Sun

2012

OMICS: A Journal of Integrative Biology

159

View full text Add to dashboard Cite

Traditional Chinese medicine (TCM) has been used for thousands of years to treat or prevent disease. The health care paradigm has shifted from a focus on disease to TCM therapy with a holistic approach. However, the actual value of TCM has not been fully recognized worldwide due to a lack of scientific approaches to its study. Today omics has become practically available, and resembles TCM in many aspects, and can serve as a key driving force for the translation of the traditional Chinese medical formulae (chinmediformulae) into practice, and will develop and advance the concept of the metabolomics of chinmediformulae (chinmedomics). Chinmedomics seeks to elucidate the therapeutic and synergistic properties and metabolism of chinmediformulae and the involved metabolic pathways using modern analytical techniques. It is an integral part of top-down systems biology, which aims to improve understanding of chinmediformulae. This approach of combining chinmedomics with chinmediformulae with modern health care systems may lead to a revolution in TCM therapy. Although the scientific study of chinmedomics is at an early stage and requires further scrutiny and validation, the approach has major implications to improve the efficacy of chinmediformulae. This article introduces and reviews the concept of chinmedomics, and highlights recent examples of the approach, which are presented for description and discussion.

show abstract

“…Metabolomics offers a new approach to the research of traditional Chinese medicines. Lu et al, who analyzed plasma from MI rats and built partial least-squares discriminate analysis (PLS-DA) models, found that MLB was able to regulate 22 identified MI biomarkers in rat plasma [56] ; this biomarker pattern was similar to the metabolomic profile of propanolol, indicating that the two drugs might have similar mechanisms. They further demonstrated that MLB exhibited a protective effect on MI mainly by decreasing the concentration of cAMP and Ca 2+ and inhibiting PKA [57] .…”

Section: Protecting Against Myocardial Ischemia and Reperfusion (I/r)mentioning

confidence: 99%

Pharmacological actions and therapeutic applications of Salvia miltiorrhiza depside salt and its active components

Wang

2012

Acta Pharmacol Sin

103

View full text Add to dashboard Cite

Salvia miltiorrhiza, a traditional medical herb known as danshen, has been widely used in China to improve blood circulation, relieve blood stasis, and treat coronary heart disease. S miltiorrhiza depside salt is a novel drug recently developed at the Shanghai Institute of Materia Medica; it contains magnesium lithospermate B (MLB) and its analogs, rosmarinic acid (RA) and lithospermic acid (LA), as active components. The drug has been used in the clinic to improve blood circulation and treat coronary heart disease. The pharmacological effects of the depside salt from S miltiorrhiza and its components have been extensively investigated. Experimental studies have demonstrated that magnesium lithospermate B possesses a variety of biological activities, especially protective effects in the cardiovascular system such as attenuation of atherosclerosis and protection against myocardial ischemia-reperfusion injury. Rosmarinic acid and lithospermic acid also show beneficial effects on the cardiovascular system. This paper reviews the recent findings regarding the mechanisms underlying the pharmacological actions of the active components of S miltiorrhiza depside salt, based on published works and our own observations.

show abstract

Metabolomic strategy to study therapeutic and synergistic effects of tanshinone IIA, salvianolic acid B and ginsenoside Rb1 in myocardial ischemia rats

Cited by 34 publications

References 17 publications

Protection of SAL B with H9C2 cells

Protection of SAL B with H9C2 cells

Future Perspectives of Chinese Medical Formulae: Chinmedomics as an Effector

Pharmacological actions and therapeutic applications of Salvia miltiorrhiza depside salt and its active components

Contact Info

Product

Resources

About