Metabolomic and chemometric study of Achras sapota L. fruit extracts for identification of metabolites contributing to the inhibition of α-amylase and α-glucosidase

Das, Susmita; Dutta, Mainak; Chaudhury, Koel; De, B. K.

doi:10.1007/s00217-015-2581-0

Cited by 36 publications

(45 citation statements)

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“…The column used was HP‐5MS capillary column (Agilent J & W) of dimensions 30m × 0.25mm × 0.25 µm. The analysis was carried out with modification (Das, Dutta, Chaudhury & De, ) of the method of Kind et al () as per users’ guide with oven temperature program (oven ramp: 1 min hold at 60°C, to 325°C at 10°C per min, 10 min hold before cool down; 37.5 min run time). The injection temperature was set at 250°C, MS transfer line at 290°C; ion source at 230°C.…”

Section: Methodsmentioning

confidence: 99%

“…Metabolomics and chemometric study have been used for identification of bioactive compounds of plants (Ali et al, 2012(Ali et al, , 2013Das et al, 2016;Maree, Kamatou, Gibbons, Viljoen, & Van Vuuren, 2014;Yuliana, Budijanto, Verpoorte, & Choi, 2013;Yuliana, Rosa, Budijanto, Verpoorte, & Choi, 2014). The identified metabolites by chemometric analysis are known as biomarkers.…”

Section: Chemometric Studymentioning

confidence: 99%

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Metabolomics and chemometric study for identification of acetylcholinesterase inhibitor(s) from the flower extracts of Nymphaea pubescens

et al. 2018

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Inhibitors of acetylcholinesterase (AChE) are used in the treatment of Alzheimer's disease. Initial screening revealed that the edible flowers of N. pubescens inhibited AChE. This finding motivated us to identify the contributory bioactive metabolite(s) responsible for inhibition AChE using Gas Chromatography ‐ Mass Spectrometry based metabolomics and chemometric approach. The metabolite levels as well as AChE inhibitory activity varied in different flower samples collected from different districts of West Bengal in the monsoon season. Different multivariate analyses differentiated the extracts with low activity (< 45% inhibition) from that of higher activities (> 45% inhibition). Chemometric analysis revealed gallic acid, a previously reported metabolite, to be one of the contributors significantly related to the inhibition of AChE by the flower extracts. Kaempferol, although negatively correlated, inhibited AChE. A probable synergistic action of metabolites is suggested for the AChE inhibitory property of N. pubescens flower extracts. Practical applications Nymphaea pubescens flower is edible in different countries. The flowers also have different medicinal properties. Acetylcholinesterase inhibitors are used in the treatment of Alzheimer's disease. As the flower extracts of this plant inhibited acetylcholinesterase, although the activity of the flower extracts varied with locality of collection. The level of metabolites also varied. A detailed study was made to identify the active constituents. Gallic acid and kaempferol were found to have high acetylcholinesterase inhibitory activities. The study suggested that consumption of this edible part of the plant may have beneficiary effect on memory function.

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Section: Methodsmentioning

confidence: 99%

Section: Chemometric Studymentioning

confidence: 99%

Metabolomics and chemometric study for identification of acetylcholinesterase inhibitor(s) from the flower extracts of Nymphaea pubescens

et al. 2018

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“…GC-MS analysis was done in gas chromatography system (Agilent 5975C, USA) following the protocol detailed by Das et al [20] using HP-5MS capillary column (length 30 m plus Duraguard 10 m, film 0.25 μm, diameter 0.25 mm narrow bore). Samples (1 μl) were inserted via the split mode (ratio 1:5) onto the GC column.…”

Section: Methodsmentioning

confidence: 99%

“…Samples (1 μl) were inserted via the split mode (ratio 1:5) onto the GC column. Metabolites were identified by comparing the fragmentation patterns of the mass spectra and retention times (Rt) with those present in Agilent Fiehn Metabolomics library using Agilent retention time locking method [20]. Automated mass spectral de-convolution and identification system was used to de-convolute GC-MS results and to categorize chromatographic peaks.…”

Section: Methodsmentioning

confidence: 99%

Sansevieria roxburghiana Schult. & Schult. F. (Family: Asparagaceae) Attenuates Type 2 Diabetes and Its Associated Cardiomyopathy

et al. 2016

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BackgroundSansevieria roxburghiana Schult. & Schult. F. (Family: Asparagaceae) rhizome has been claimed to possess antidiabetic activity in the ethno-medicinal literature in India. Therefore, present experiments were carried out to explore the protective role of edible (aqueous) extract of S. roxburghiana rhizome (SR) against experimentally induced type 2 diabetes mellitus (T2DM) and its associated cardiomyopathy in Wistar rats.MethodsSR was chemically characterized by GC-MS analysis. Antidiabetic activity of SR (50 and 100 mg/kg, orally) was measured in high fat diets (ad libitum) + low-single dose of streptozotocin (35 mg/kg, intraperitoneal) induced type 2 diabetic (T2D) rat. Fasting blood glucose level was measured at specific intermissions. Serum biochemical and inflammatory markers were estimated after sacrificing the animals. Besides, myocardial redox status, expressions of signal proteins (NF-κB and PKCs), histological and ultrastructural studies of heart were performed in the controls and SR treated T2D rats.ResultsPhytochemical screening of the crude extract revealed the presence of phenolic compounds, sugar alcohols, sterols, amino acids, saturated fatty acids within SR. T2D rats exhibited significantly (p < 0.01) higher fasting blood glucose level with respect to control. Alteration in serum lipid profile (p < 0.01) and increased levels of lactate dehydrogenase (p < 0.01) and creatine kinase (p < 0.01) in the sera revealed the occurrence of hyperlipidemia and cell destruction in T2D rats. T2DM caused significant (p < 0.05–0.01) alteration in the biochemical markers in the sera. T2DM altered the redox status (p < 0.05–0.01), decreased (p < 0.01) the intracellular NAD and ATP concentrations in the myocardial tissues of experimental rats. While investigating the molecular mechanism, activation PKC isoforms was observed in the selected tissues. T2D rats also exhibited an up-regulation in nuclear NF-κB (p65) in the cardiac tissues. So, oral administration of SR (50 and 500 mg/kg) could reduce hyperglycemia, hyperlipidemia, membrane disintegration, oxidative stress, vascular inflammation and prevented the activation of oxidative stress induced signaling cascades leading to cell death. Histological and ultra-structural studies of cardiac tissues supported the protective characteristics of SR.ConclusionsFrom the present findings it can be concluded that, SR could offer protection against T2DM and its associated cardio-toxicity via multiple mechanisms viz. hypoglycemic, antioxidant and anti-inflammatory actions.

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“…Furthermore, the tannin moiety size, structure distribution and category vary widely with fruit sources (Tessmer, Besada, Hernando, Appezzato-da-Glória, Quiles, & Salvador, 2016) and are expected to affect the possibility and intensity of amylase-tannin interactions (Amoako & Awika, 2016). In addition to tannins, some phenolic acids in fruits, like ellagitannin acid, gallic acid and chlorogenic acid have also been reported to have inhibitory activities against α-amylase (Das, Dutta, Chaudhury, & De, 2016;Mohammeda, Gbonjubola, Koorbanally, & Islam, 2017), as well as some flavonoids (Liu, Luo, Li, She, & Gao, 2017) and procyanidins (Yilmazer-Musa, Griffith, Michels, Schneider, & Frei, 2012). The different polyphenolic components in fruits may affect different steps in starch digestion in a synergistic manner.…”

Section: Teamentioning

confidence: 99%

Studying the interactions between tea polyphenols and α-amylase

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Dixon, Cornish-Bowden and Lineweaver-Burk plot analyses were applied to study the detailed kinetics of inhibition of porcine pancreatic α-amylase (PPA) by tea polyphenols.Fluorescence quenching (FQ), differential scanning calorimetry (DSC) and isothermal titration calorimetry (ITC) were combined with the kinetics of inhibition to elucidate the mechanism of binding interactions of tea polyphenols and PPA. Then, the reciprocal of competitive inhibition constant (1/K ic ), fluorescence quenching constant (K FQ ) and binding constant (K itc ) obtained from these measurements were compared and correlated to analyse the relations between PPA inhibition and binding behaviour. The role of the galloyl moiety in binding of catechins and theaflavins with PPA was highlighted as well. In addition, the effects of three soluble polysaccharides (citrus pectin (CP), wheat arabinoxylan (WAX) and oat β-glucan (OBG)) on the inhibition of PPA by tea polyphenols were studied through PPA inhibition, IC 50 value, inhibition kinetics and fluorescence quenching methods. Furthermore, the effects of tea polyphenols on binding of PPA with normal maize starch granules were studied in terms of binding ratio, dissociation constant (K d ) and binding rate. Then, the association constant (1/K d ), 1/K ic and K FQ of tea polyphenols were correlated to analyse the effects of PPA inhibition on the binding of the enzyme with starch.The results show that green, oolong and black tea extracts, epigallocatechin gallate, theaflavin-3, 3'-digallate and tannic acid were competitive inhibitors of PPA, whereas epicatechin gallate, theaflavin-3'-gallate and theaflavin were mixed-type inhibitors with both competitive and uncompetitive inhibitory characteristics. Only catechins with a galloyl substituent at the 3-position showed measurable inhibition. K ic values were lower for theaflavins than catechins, with the lowest value for theaflavin-3, 3'-digallate. The lower K ic than the uncompetitive inhibition constant for the mixed-type inhibitors suggests that they bind more tightly with free PPA than with the PPA-starch complex. A 3 and/or 3'-galloyl moiety in catechin and theaflavin structures was consistently found to increase inhibition of PPA through enhanced association with the enzyme active site. A higher quenching effect of polyphenols corresponded to a stronger inhibitory activity against PPA. The red-shift of maximum emission wavelength of PPA bound with some polyphenols indicated a potential IV

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Metabolomic and chemometric study of Achras sapota L. fruit extracts for identification of metabolites contributing to the inhibition of α-amylase and α-glucosidase

Cited by 36 publications

References 20 publications

Metabolomics and chemometric study for identification of acetylcholinesterase inhibitor(s) from the flower extracts of Nymphaea pubescens

Metabolomics and chemometric study for identification of acetylcholinesterase inhibitor(s) from the flower extracts of Nymphaea pubescens

Sansevieria roxburghiana Schult. & Schult. F. (Family: Asparagaceae) Attenuates Type 2 Diabetes and Its Associated Cardiomyopathy

Studying the interactions between tea polyphenols and α-amylase

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