Metabolomic analysis of serum reveals the potential effective ingredients and pathways of Danggui Buxue Tang in promoting erythropoiesis

Wang, Xiao; Bei, Haikang; Du, Ruilan; Chen, Qizhu; Wu, Fenglin; Bo, Huaben

doi:10.1016/j.ctim.2019.102247

Cited by 11 publications

(5 citation statements)

References 27 publications

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“…Studies have shown that combined ASR and AR could stimulate the growth and inhibit the apoptosis of bone marrow stromal cells [ 15 , 29 ]. In addition, the number of RET in peripheral blood was significantly increased, the secretion of haematopoietic growth factor and the proliferation of haematopoietic progenitor cells were promoted in mice receiving the herbs [ 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Chinese Herbal Medicine Significantly Impacts the Haematological Variables of the Athlete Biological Passport

Chang

Wang

Liu

et al. 2021

IJERPH

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In the fight against sports doping, the Athlete Biological Passport (ABP) system aims to indirectly unveil the doping incidents by monitoring selected biomarkers; however, several unexplored extrinsic factors may dampen a fair interpretation of ABP profiles. Chinese herbal medicine (CHM) plays a pivotal role in the health care system, and some remedies have a long history of being used to treat anaemia. In this study, we addressed the concerns of whether the CHM administration could yield a measurable effect on altering the ABP haematological variables. Forty-eight healthy volunteers were randomly assigned to receive two-week oral administration of one of the six selected CHM products that are commonly prescribed in Taiwan (eight subjects per group). Their blood variables were determined longitudinally in the phases of baseline, intervention, and recovery over 5 weeks. Blood collection and analyses were carried out in strict compliance with relevant operating guidelines. In the groups receiving Angelicae Sinensis Radix, Astragali Radix, and Salviae Miltiorrhizae Radix et Rhizoma, a significant increased reticulocyte percentage and decreased OFF-hr Score were manifested during the intervention, and such effects even sustained for a period of time after withdrawal. All other variables, including haemoglobin and Abnormal Blood Profile Score, did not generate statistical significance. Our results show that the use of CHM may impact the ABP haematological variables. As a consequence, we recommend athletes, particularly those who have been registered in the testing pool, should be aware of taking specific Chinese herbal-based treatment or supplementation, and document any of its usage on the anti-doping forms.

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Section: Discussionmentioning

confidence: 99%

Chinese Herbal Medicine Significantly Impacts the Haematological Variables of the Athlete Biological Passport

Chang

Wang

Liu

et al. 2021

IJERPH

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“…DBD is a simple formula which consists of two botanical drugs: Radix Angelicae Sinensis (Danggui, DG) and Radix Astragali (Huangqi, HQ) with a weight ratio of 1: 5. A large number of studies have shown that DBD remarkably increases the number of white blood cells, reticulocytes, and bone marrow nucleated cells ( Liu et al, 2010 ; Wang et al, 2020 ). DBD also significantly affects hematopoietic function by promoting bone tissue regeneration, regulating immune-mediated aplastic anemia, and modulating gut microbiota balance ( Yang et al, 2014 ; Wang et al, 2015 ; Du et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Integrated transcriptomic and metabolomic profiles reveal the protective mechanism of modified Danggui Buxue decoction on radiation-induced leukopenia in mice

Chen¹,

Xin

Wei

et al. 2023

Front. Pharmacol.

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Leukopenia caused by radiation hinders the continuous treatment of cancers. Danggui Buxue Decoction (DBD) has been widely used in clinical owing to low toxicity and definite therapeutic effects to increase leukocytes. Meanwhile, icaritin (ICT) has also been proved to have the effect of boosting peripheral blood cells proliferation. However, there is no study to prove the efficacy of MDBD (Modified Danggui Buxue Decoction), a derivative herbal formula composed of DBD and ICT, in the treatment of radiation-induced leukopenia. In this study, we performed a model of 3.5 Gy whole-body radiation to induce leukopenia in mice. The results of pharmacodynamic studies demonstrated that MDBD could significantly increase the white blood cells in peripheral blood by improving the activity of bone marrow nuclear cells, reducing bone marrow damage, modulating spleen index, and regulating hematopoietic factors to alleviate leukopenia. We also analyzed the integrated results of metabolomics and transcriptomics and found that MDBD could relieve leukopenia and alleviate bone marrow damage by targeting steroid biosynthesis and IL-17 signaling pathway, in which the key genes are Jun, Cxcl2 and Egr1. Therefore, our study provides a basis for the effectiveness and compatibility in the combination of traditional Chinese medicine formula and small molecule drugs.

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“…It is composed of Radix Astragali and Angelica sinensis and replenishes Qi and promotes the production of blood ( 9 ). Modern studies have confirmed that DBD can promote angiogenesis ( 10 ), improve bone marrow hematopoietic function ( 11 ), enhance non-specific immunity ( 12 ) and improve immune regulation in a pathological injury model ( 13 ). The intestinal tract is not only a place for digestion and absorption of nutrients but also has important immune functions.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of intestinal mucosal immunity and immune response to the foot-and-mouth disease vaccine by oral administration of danggui buxue decoction

et al. 2022

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This study investigated the effect of Danggui Buxue decoction (DBD) on the immunity of an O-type foot-and-mouth disease (FMD) vaccine and intestinal mucosal immunity. SPF KM mice were continuously and orally administered DBD for 5 d and then inoculated with an O-type FMD vaccine. The contents of a specific IgG antibody and its isotypes IgG1, IgG2a, IgG2b, and IgG3 in serum and SIgA in duodenal mucosa were determined by ELISA at 1 and 3 W after the 2nd immunization. qRT-PCR was used to detect mRNA expression levels of IL-4, IL-10, IFN-γ, and IL-33 in the spleen, and mRNA expression levels of J-chain, pIgR, BAFF, APRIL, IL-10, IFN-γ and IL-33 in the duodenum. The results showed that compared with the control group, oral administration of DBD significantly increased levels of the anti-FMD virus (FMDV)-specific antibodies IgG, IgG1, and IgG2a in the serum of O-type FMD vaccine-immunized mice 1 W after the 2nd immunization (P < 0.05), upregulated mRNA expression levels of spleen lymphocyte cytokines IL-4 and IL-33 (P < 0.05), promoted the secretion of SIgA in duodenal mucosa (P < 0.05). The mRNA expression levels of J-chain, pIgR, BAFF, APRIL, IL-10, and IL-33 in duodenal tissues were upregulated (P < 0.05). This study indicates that DBD has a good promotion effect on the O-type FMD vaccine and the potential to be an oral immune booster.

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Metabolomic analysis of serum reveals the potential effective ingredients and pathways of Danggui Buxue Tang in promoting erythropoiesis

Cited by 11 publications

References 27 publications

Chinese Herbal Medicine Significantly Impacts the Haematological Variables of the Athlete Biological Passport

Chinese Herbal Medicine Significantly Impacts the Haematological Variables of the Athlete Biological Passport

Integrated transcriptomic and metabolomic profiles reveal the protective mechanism of modified Danggui Buxue decoction on radiation-induced leukopenia in mice

Enhancement of intestinal mucosal immunity and immune response to the foot-and-mouth disease vaccine by oral administration of danggui buxue decoction

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