Metabolite Profiles for Antrodia cinnamomea Fruiting Bodies Harvested at Different Culture Ages and from Different Wood Substrates

Abstract

Antrodia cinnamomea is a precious edible fungus endemic to Taiwan that has long been used as a folk remedy for health promotion and for treating various diseases. In this study, an index of 13 representative metabolites from the ethanol extract of A. cinnamomea fruiting body was established for use in quality evaluation. Most of the index compounds selected, particularly the ergostane-type triterpenoids and polyacetylenes, possess good anti-inflammation activity. A comparison of the metabolite profiles of different ethanol extracts from A. cinnamomea strains showed silmilar metabolites when the strains were grown on the original host wood (Cinnamomum kanehirai) and harvested after the same culture time period (9 months). Furthermore, the amounts of typical ergostane-type triterpenoids in A. cinnamomea increased with culture age. Culture substrates also influenced metabolite synthesis; with the same culture age, A. cinnamomea grown on the original host wood produced a richer array of metabolites than A. cinnamomea cultured on other wood species. We conclude that analysis of a fixed group of compounds including triterpenoids, benzolics, and polyacetylenes constitutes a suitable, reliable system to evaluate the quality of ethanol extract from A. cinnamomea fruiting bodies. The evaluation system established in this study may provide a platform for analysis of the products of A. cinnamomea.

Genomic and transcriptomic analyses of the medicinal fungusAntrodia cinnamomeafor its metabolite biosynthesis and sexual development

“…The lanostane-type triterpenoids are produced in both mycelium and basidium, whereas ergostanes are related to basidiomatal formation and are produced only in basidium (6,14). Significantly, the ergostane production of A. cinnamomea growing on C. kanehirai Hayata is of higher efficiency and shows more compound variety than that growing on other trees (14,15). As to the metabolites of mycelium, lanostane-type triterpenoids and the derivatives of maleic acid, succinic acid and ubiquinone, have been reported (15).…”

Qualitative and quantitative analysis of seven signature components in the fruiting body of Antrodia cinnamomea by HPLC—ESI—MS/MS

“…Previously, Lin et al had reported the quantitative determination method of AC by HPLC with ultraviolet detection, requiring a long HPLC running time [17]. HPLC with mass spectrometry detection to evaluate the quality of AC mycelium was also conducted by Zhao and Leung, but the metabolite composition of the fruiting bodies of AC has not been evaluated [31].…”

“…Highperformance liquid chromatography (HPLC) with ultraviolet (UV) and photodiode array (PDA) detection methods has become a routine practice in some laboratories to quantify benzenoids and triterpenoids derivatives of fruiting body, mycelia, extract, and commercial preparation of AC [16][17][18][19]. These methods have played important roles in the quality control of AC and its commercial products.…”

Effect of cultural conditions on antrodin C production by basidiomycete Antrodia camphorata in solid‐state fermentation

“…C), there was almost no typical triterpenoids in the mycelial product. Lin et al argued that the presence of C. kanehirai was a necessary condition for the production of ergostan‐class triterpenoids in A. camphorata . Although there were no ergostan‐class triterpenoids in the mycelial product of A. camphorata produced by solid‐state fermentation in this study, several new active compounds were produced that may make compensate for the lack of fruiting body to a certain degree .…”