Metabolism of Organic Hydroperoxides

Sies, Helmut; Wendel, Albrecht; Bors, Wolf

doi:10.1016/b978-0-12-380060-2.50022-2

Cited by 16 publications

(17 citation statements)

References 81 publications

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“…1). The total amount of ascorbate in human liver lies in the range of ϳ700 nmol/g wet weight (46), whereas the total amount of NAD(P)H in rat liver was found in the range of ϳ400 nmol/g wet weight (58). Therefore, it can be estimated that 75-90% of the intracellular NAD(P)H can be protected by intracellular ascorbate against the attack of ⅐ NO/O 2 .…”

Section: Discussionmentioning

confidence: 97%

Ascorbate Is a Potent Antioxidant against Peroxynitrite-induced Oxidation Reactions

Kirsch

Groot

2000

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 97%

Ascorbate Is a Potent Antioxidant against Peroxynitrite-induced Oxidation Reactions

Kirsch

Groot

2000

Journal of Biological Chemistry

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“…To the contrary, the total amount of NAD(P)H in rat liver cytosol was found in the range of 90 nmol/g wet weight (43), whereas the bulk of NAD(P)H is located in the mitochondria with a total amount up to ϳ0.4 mol/g wet weight (43), i.e. ϳ20 times lower than the total amount of GSH.…”

Section: Discussionmentioning

confidence: 99%

Reaction of Peroxynitrite with Reduced Nicotinamide Nucleotides, the Formation of Hydrogen Peroxide

Kirsch

Groot

1999

Journal of Biological Chemistry

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“…To detect any changes in the rough estimates of intramitochondrial free Ca2+, by reduced nicotinamide nucteotide pool, the endogenous using the following assumptions: (1) mitochondria NAD(P)H fluorescence was measured. As discussed by occupy 50 of the total cell volume, as in pig lymphocytes Sies [19], the fluorescence signal will include contributions [20]; (2) Similarly, the stimulation of respiration in perfused rat liver by phenylephrine [24] or glucagon [25] was reported to be blocked by depleting intracellular calcium pools. McCormack's work [4,5] has demonstrated that hormones which are known to mobilize Ca2" and stimulate respiration in intact cells also increase mitochondrial calcium and thereby activate intramitochondrial dehydrogenases.…”

Section: Resultsmentioning

confidence: 99%

Stimulation of respiration by mitogens in rat thymocytes is independent of mitochondrial calcium

Lakin-Thomas

Brand

1988

Biochemical Journal

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The role of calcium in the control of respiration by the mitogen concanavalin A (ConA) was investigated in rat thymocytes. ConA induced an increase in both mitochondrial respiration and the mitochondrial calcium pool. The stimulation of respiration was shown to be independent of the increase in mitochondrial calcium: the calcium pool declined after 3 min, whereas the respiration increase was persistent, and was not affected by depletion of the calcium pool or by buffering intracellular Ca2" transients with quin2. The mitogen phytohaemagglutinin stimulated respiration to the same extent as ConA, but did not increase the mitochondrial calcium pool. In addition, respiration was unaffected by changes in the mitochondrial calcium pool induced by increasing or decreasing extracellular calcium. These results indicate that control of respiration is not located in the Ca2"-sensitive mitochondrial dehydrogenases. The ConA-induced increase in respiration could be blocked by oligomycin, suggesting control by cytoplasmic ATP turnover, and was not associated with detectable changes in NAD(P)H fluorescence, indicating a balance between increased electron transfer and increased supply of reduced substrates. INTRODUCTIONThe factors which control respiration rate, and the mechanisms by which changes in respiration rate are brought about, have not been thoroughly described for any intact cell system. Comparatively more is known about the control of respiration in isolated mitochondria; when this information is extrapolated to intact cells, it can be argued that respiration is probably controlled by relatively few factors: the kinetic properties of the components of oxidative phosphorylation, the supply of substrates (NADH, reduced ubiquinone and 02) and the phosphorylation state of the adenine nucleotides (ATP/ ADP ratio) (for review, see [11).One mechanism for controlling the supply of reduced substrates is by controlHing the activity of intramitochondrial dehydrogenases. Denton & McCormack [2,3]

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Metabolism of Organic Hydroperoxides

Cited by 16 publications

References 81 publications

Ascorbate Is a Potent Antioxidant against Peroxynitrite-induced Oxidation Reactions

Ascorbate Is a Potent Antioxidant against Peroxynitrite-induced Oxidation Reactions

Reaction of Peroxynitrite with Reduced Nicotinamide Nucleotides, the Formation of Hydrogen Peroxide

Stimulation of respiration by mitogens in rat thymocytes is independent of mitochondrial calcium

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