Metabolic fingerprinting of high-fat plasma samples processed by centrifugation- and filtration-based protein precipitation delineates significant differences in metabolite information coverage

Barri, Thaer; Holmer-Jensen, Jens; Hermansen, Kjeld; Dragsted, Lars Ove

doi:10.1016/j.aca.2011.12.065

Cited by 51 publications

(42 citation statements)

References 29 publications

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“…Leucine-enkephalin (500 ng/ml) was infused as the lock-spray agent to calibrate the mass accuracy every 5 sec with 1 sec scan time. Quality control samples were used to evaluate possible contamination, monitoring the changes in mass accuracy, retention time and instrumental sensitivity drifts 79, 84 .…”

Section: Methodsmentioning

confidence: 99%

Breastmilk-promoted bifidobacteria produce aromatic amino acids in the infant gut

Laursen

Sakanaka

Burg

et al. 2020

Preprint

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26Breastfeeding profoundly shapes the infant gut microbiota, which is critical for early life immune 27 development. However, few breastmilk-dependent microbial metabolites mediating host-microbiota 28 interactions are currently known. We here demonstrate that breastmilk-promoted Bifidobacterium 29 species convert aromatic amino acids (tryptophan, phenylalanine and tyrosine) into their respective 30 aromatic lactic acids (indolelactate, phenyllactate and 4-hydroxyphenyllactate) via a previously 31 unrecognised aromatic lactate dehydrogenase. By longitudinal profiling of the gut microbiota 32 composition and metabolome of stool samples of infants obtained from birth until 6 months of age, 33 we show that stool concentrations of aromatic lactic acids is determined by the abundance of human 34 milk oligosaccharide degrading Bifidobacterium species containing the aromatic lactate 35 dehydrogenase. Finally, we demonstrate that stool concentrations of Bifidobacterium-derived 36 indolelactate are associated with the capacity of infant stool samples to activate the aryl 37 hydrocarbon receptor, a receptor important for maintenance of intestinal homeostasis and immune 38 system development. These findings open up new directions towards understanding the role of 39 breastmilk-promoted Bifidobacterium in mediating host-microbiota interactions in early life. 40 INTRODUCTION 41Human breastmilk is a perfectly adapted nutritional supply for the infant 1 . Breastfeeding provides 42 children with important short-term protection against infections, and may also provide long-term 43 metabolic benefits 1,2 . These benefits may partly be mediated through the gut microbiota, since 44 breastfeeding is the strongest determinant of gut microbiota composition and function during 45 infancy 3-5 . Human breastmilk contains human milk oligosaccharides (HMOs), which are complex, 46 highly abundant sugars serving as substrates for specific microbes including certain species of 47 Bifidobacterium 6 . This co-evolution between bifidobacteria and the host, mediated by HMOs, to a 48 large extent directs the colonization of the gut in early life, which has critical impact on the immune 49 system 7 . Depletion of specific microbes, including Bifidobacterium, in early life has been associated 50 with increased risk of allergy and asthma development in childhood 8,9 , and is suggested to 51 compromise immune function and lead to increased susceptibility to infectious disease 10,11 . Despite 52 Bifidobacterium dominating the gut of breastfed infants and being widely acknowledged as 53 beneficial, mechanistic insights on the contribution of these bacteria and their metabolites to 54 immune development during infancy remain limited. Recent studies show that microbial aromatic 55 amino acid metabolites including tryptophan-derived indoles 12 , via activation of the aryl 56 hydrocarbon receptor (AhR), can fortify the intestinal barrier 13,14 , protect against pathogenic 57 infections 15,16 and influence host metabolism 13,17,18 , which makes this...

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Section: Methodsmentioning

confidence: 99%

Breastmilk-promoted bifidobacteria produce aromatic amino acids in the infant gut

Laursen

Sakanaka

Burg

et al. 2020

Preprint

Self Cite

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“…Ion source and desolvation gas (nitrogen) temperatures were set at 120 and 400°C, respectively. More detailed UPLC-QTOF analysis conditions were explained previously [15]. Blanks (5% of acetonitrile∶methanol 70∶30 v/v in water) and external metabolomics standard mixtures were injected every 30 plasma samples throughout each analytical batch.…”

Section: Methodsmentioning

confidence: 99%

Effect of trans Fatty Acid Intake on LC-MS and NMR Plasma Profiles

et al. 2013

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BackgroundThe consumption of high levels of industrial trans fatty acids (TFA) has been related to cardiovascular disease, diabetes and sudden cardiac death but the causal mechanisms are not well known. In this study, NMR and LC-MS untargeted metabolomics has been used as an approach to explore the impact of TFA intake on plasma metabolites.Methodology/Principal FindingsIn a double-blinded randomized controlled parallel-group study, 52 overweight postmenopausal women received either partially hydrogenated soybean oil, providing 15.7 g/day of TFA (trans18:1) or control oil with mainly oleic acid for 16 weeks. Subsequent to the intervention period, the subjects participated in a 12-week dietary weight loss program. Before and after the TFA intervention and after the weight loss programme, volunteers participated in an oral glucose tolerance test. PLSDA revealed elevated lipid profiles with TFA intake. NMR indicated up-regulated LDL cholesterol levels and unsaturation. LC-MS profiles demonstrated elevated levels of specific polyunsaturated (PUFA) long-chain phosphatidylcholines (PCs) and a sphingomyelin (SM) which were confirmed with a lipidomics based method. Plasma levels of these markers of TFA intake declined to their low baseline levels after the weight loss program for the TFA group and did not fluctuate for the control group. The marker levels were unaffected by OGTT.Conclusions/SignificanceThis study demonstrates that intake of TFA affects phospholipid metabolism. The preferential integration of trans18:1 into the sn-1 position of PCs, all containing PUFA in the sn-2 position, could be explained by a general up-regulation in the formation of long-chain PUFAs after TFA intake and/or by specific mobilisation of these fats into PCs. NMR supported these findings by revealing increased unsaturation of plasma lipids in the TFA group. These specific changes in membrane lipid species may be related to the mechanisms of TFA-induced disease but need further validation as risk markers.Trial registrationRegistered at clinicaltrials.gov as NCT00655902

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“…In order to improve the utilization absorption rate of calcium, chelating method was adopted. Ethylenediaminetetraacetic acid and amino acid are ideal carrier of Ca 2+ due to their strong chelating ability [7,8]. However, due to the amount and cost of chelating agents, the range of their use is limited, especially in agriculture, where high costs do not allow them to be used in crops with low economic value.…”

Section: Introductionmentioning

confidence: 99%

A simple method for preparing chelated calcium based on the black liquor generated by the pulp and paper industry

Yang

Cheng

Liu

et al. 2019

Environmental Pollutants and Bioavailability

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A simple method for preparing chelated calcium from black liquor produced in the paper industry was studied. Various factors influencing the chelation rate of Ca 2+ were studied, including the system pH, reaction temperature, reaction time, reaction solution ratio. The optimum reaction conditions are as follows: the system pH, reaction temperature, reaction solution ratio (V(CaCl 2 ):m (Black liquor)) and time were 5.6, 25°C, 1:20 and 60 min, respectively. The maximum Ca 2+ chelation rate was 88.75%. The characteristics of chelated calcium fertilizer in black liquor were studied by Fourier transform infrared, scanning electron microscope . The results indicated that the CaCl 2 could be chelated by the black liquor from papermaking industry, and showed a high chelating rate. ARTICLE HISTORY

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Metabolic fingerprinting of high-fat plasma samples processed by centrifugation- and filtration-based protein precipitation delineates significant differences in metabolite information coverage

Cited by 51 publications

References 29 publications

Breastmilk-promoted bifidobacteria produce aromatic amino acids in the infant gut

Breastmilk-promoted bifidobacteria produce aromatic amino acids in the infant gut

Effect of trans Fatty Acid Intake on LC-MS and NMR Plasma Profiles

A simple method for preparing chelated calcium based on the black liquor generated by the pulp and paper industry

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