Metabolic and antioxidant profiles of herbal infusions and decoctions

Fotakis, Charalambos; Tsigrimani, Diamantina; Tsiaka, Thalia; Lantzouraki, Dimitra Z.; Strati, Irini F.; Makris, Constantinos; Tagkouli, Dimitra; Proestos, Charalampos; Sinanoglou, Vassilia J.; Zoumpoulakis, Panagiotis

doi:10.1016/j.foodchem.2016.05.124

Cited by 53 publications

(34 citation statements)

References 29 publications

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“…In addition, ME was characterized by NMR spectroscopy data exploited for primary and secondary metabolites identification and quantification (including non-ionizable and non-volatile compounds) following the approach developed for the analysis of complex plant extracts (Amigoni et al, 2017;Palmioli et al, 2017;Ciaramelli et al, 2018;Ciaramelli et al, 2019;. The identification of metabolites was based on mono and bidimensional NMR spectra analysis and is in agreement with data from previous literature (Brahmi et al, 2015;Fotakis et al, 2016). After the manual identification of compounds, a specific library was built using the Simple Mixture Analysis (SMA) tool implemented in the MestReNova 14.1 software.…”

Section: Mint Extract Gc/ms and Nmr Analysissupporting

confidence: 53%

Anticancer Effects of Wild Mountain Mentha longifolia Extract in Adrenocortical Tumor Cell Models

et al. 2020

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Mint [Mentha longifolia (L.) Hudson] is an aromatic plant that belongs to Lamiaceae family. It is traditionally used as herbal tea in Europe, Australia and North Africa and shows numerous pharmacological effects, such as spasmolytic, antioxidant, antimicrobial and anti-hemolytic. Recently, its antiproliferative role has been suggested in a small number of tumor cell models, but no data are available on adrenocortical carcinoma, a malignancy with a survival rate at 5 years of 20%-30% which frequently metastasize. This work aimed to study the effects of Mentha longifolia L. crude extract (ME) on two adrenocortical tumor cell models (H295R and SW13 cells). Chemical composition of ME was assessed by gaschromatography/mass spectrometry and NMR spectroscopy analysis. Brine shrimp lethality assay showed ME effects at >0.5 µg/µl (p < 0.05). Cell viability and vitality were determined by MTT, SRB, and trypan blue assays in H295R and SW13 cells. The antiproliferative effects of ME were more evident in SW13 cells at 72 h (ME > 0.5 µg/µl, p < 0.05). Combination of ME with mitotane (approved drug for adrenocortical carcinoma) seemed not to reinforce the efficacy of the herb. As control, human fibroblasts were treated with ME with no effect on cell viability. Clonogenic assay was concordant with previous cell viability tests (ME > 0.5 µg/µl, p < 0.05), while Wright staining demonstrated the presence of both necrotic and apoptotic cells. Cell cycle analysis showed a strong increase in subG0/G1 phase, related to cell death. Furthermore, MAPK and PI3k/Akt pathways were modulated by Western blot analysis when treating cells with ME alone or combined with mitotane. The crude methanolic extract of wild mountain mint can decrease cell viability, vitality and survival of adrenocortical tumor cell models, in particular of SW13 cells. These data show the potential anticancer effects of ME, still more work is needed to corroborate these findings.FIGURE 7 | Representative Western blot analysis for SW13 and H295R cells. Lines 1 and 5: untreated control. Lines 2 and 6: 0.5 mg/ml ME. Lines 3 and 7: mitotane 10 mM. Lines 4 and 8: ME 0.5 mg/ml + mitotane 10 mM. Experiments performed in triplicate.Patti et al.

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Section: Mint Extract Gc/ms and Nmr Analysissupporting

confidence: 53%

Anticancer Effects of Wild Mountain Mentha longifolia Extract in Adrenocortical Tumor Cell Models

et al. 2020

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“…Decoctions are one of the most consumed drinkable forms of plants (10). For this reason, the fruit extracts were obtained by using decoction method.…”

Section: Preparation Of Extractsmentioning

confidence: 99%

In vitro Antidiabetic Activities of Two Sorbus Species

Hasbal¹,

Yilmaz-Ozden²,

Can³

2017

Eur J Biol

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Fruits of several Sorbus species (Rosaceace) are used both in traditional medicine as antidiabetic, antiinflammatory, diuretic, vasoprotective and in foods. In this study, in vitro antidiabetic activities of water extracts of Sorbus aucuparia L. (rowan tree) and Sorbus torminalis L. Crantz (wild sevice tree) fruits were investigated by measuring inhibitory potentials on α-glucosidase and pancreatic α-amylase activities, the most important digestive enzymes. Also, the total phenolic and flavonoid contents of the fruits were determined to evaluate the association between phenolic content and antidiabetic activity. S. torminalis and S. aucuparia extracts exhibited strong α-glucosidase inhibitory activity, more effective than that of standard drug acarbose. However, S. torminalis has shown moderate inhibitory effect against α-amylase while S. aucuparia exhibited weak inhibition. The total phenolic and flavonoid contents of the fruits were correlated with andiabetic activities. It has been suggested that antidiabetic effects of the fruits may be due to phenolic compounds present therein. Therefore, S. aucuparia and S. torminalis fruits might be potential sources of antidiabetic compounds.

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“…Both of the ultrasonic and microwave-assisted Soxhlet extraction have enormously reduced the cycle of extraction but in term of the extraction yield, the conventional Soxhlet extraction produced the highest result compared to both of it for one-stage extraction [27]. It is is the main reason conventional Soxhlet extraction was chosen to be used in this experimental worksextraction by boiling also known as decoction that involves heating of the solvent which extracts more compounds from the samples, while soaking or infusion is extraction without applying the heat [28].…”

Section: Introductionmentioning

confidence: 99%

Tannin Extraction from Bark of Rhizophora Mucronata Using Soxhlet and Boiling Techniques

Muhayyidin

Ghazali

Bakar

et al. 2018

Int. J. Adv. Sci. Eng. Inf. Technol.

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Extraction of tannin Rhizophora Mucronata barks by using two methods, which were soxhlet extraction and boiling were demonstrated in this study in order to evaluate the effective technique for extraction. Several parameters such as the type of solvent used, time of extraction, and sample size were specified. The extractant was then evaporated using vacuum rotary evaporator. The percentage of extractives and the characterization of condensed (Stiasny Index) and hydrolyzable tannins were all quantified. The milled barks with different sizes were introduced to the extraction using water, methanol, acetone, and toluene as a solvent for three, six and nine hours for Soxhlet extraction while for boiling only water were used as a solvent and at four, six and eight hours. Results demonstrated that extraction from the bark of Rhizophora Mucronata with water for six and nine hours extracted approximately 29 wt% and 13wt % for both soxhlet extraction and boiling respectively when coupled with vacuum rotary evaporator. The Soxhlet extraction method was superior as compared to boiling. The optimum parameters for the Soxhlet extraction were water as the solvent at six hours extraction with 0.5 mm of milled bark particles size that extracted 29 wt% of extractives. The condensed tannin and hydrolyzable tannin of the extractives using soxhlet extraction were approximately 76 wt% and 0.00133 wt% respectively.

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Metabolic and antioxidant profiles of herbal infusions and decoctions

Cited by 53 publications

References 29 publications

Anticancer Effects of Wild Mountain Mentha longifolia Extract in Adrenocortical Tumor Cell Models

Anticancer Effects of Wild Mountain Mentha longifolia Extract in Adrenocortical Tumor Cell Models

In vitro Antidiabetic Activities of Two Sorbus Species

Tannin Extraction from Bark of Rhizophora Mucronata Using Soxhlet and Boiling Techniques

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