Meta-topolin, a highly active aromatic cytokinin from poplar leaves (Populus × canadensis Moench., cv. Robusta)

Strnad, Miroslav; Hanuš, Jan; Vaněk, Tomáš; Kamínek, M.; Ballantine, James A.; Fussell, Brynley; Hanke, David E.

doi:10.1016/s0031-9422(96)00816-3

Cited by 126 publications

(59 citation statements)

References 19 publications

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“…Letham (1971) reported that cytokinins can promote markedly the expansion of radish cotyledons and attributed this response to the promotion of cell enlargement. Meta-topolin is an active aromatic cytokinin (Strnad et al 1997) and stimulated cotyledon growth markedly.…”

Section: Resultsmentioning

confidence: 99%

“…Plant . Meta-topolin (mT) has been established as an active aromatic cytokinin by Strnad et al (1997). The present investigation assessed the effects of mT on radish cotyledon growth.…”

mentioning

confidence: 99%

“…The effects of kinetin, benzyladenine (BA) and its riboside have been documented on radish cotyledons. Meta-topolin has been etablished as an active aromatic cytokinin by Strnad et al (1997). The sensitivity of the radish cotyledon bioassay to mT has not been established.…”

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confidence: 99%

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Growth responses of excised radish cotyledons to meta-topolin

Palavan-Ünsal¹,

Çağ²,

Çetin³

2002

Can. J. Plant Sci.

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Palavan-Ünsal, N., Çag, S. and Çetin, E. 2002. Growth responses of excised radish cotyledons to meta-topolin. Can. J. Plant . Meta-topolin (mT) has been established as an active aromatic cytokinin by Strnad et al. (1997). The present investigation assessed the effects of mT on radish cotyledon growth. The effect of mT on cotyledon growth, chlorophyll, N and soluble protein contents were measured. Peroxidase (POD) activity and polyamine (PA) contents were also determined. Treatment at 0.25, 0.5 and 1.0 mM mT concentrations resulted in 44, 50 and 57% increments in cotyledon growth, respectively, compared with control cotyledons. Increase in chlorophyll content per cotyledon was highest at low mT concentrations. Peroxidase activity was highest at 1.0 mM mT. Soluble protein content was reduced by the application of mT.Key words: Cotyledon growth, meta-topolin, polyamine, peroxidase, radish cotyledons Palavan-Ünsal, N., Çag, S. et Çetin, E. 2002. Incidence de la méta-topoline sur la croissance des cotylédons de radis excisés. Can. J. Plant Sci. 82: 191-194. Strnad et al (1997) avaient démontré que la méta-topoline (mT) agit comme une cytokinine aromatique. La présente étude devait préciser les effets de la mT sur la croissance des cotylédons de radis. Les auteurs ont mesuré l'incidence de la mT sur la croissance des cotylédons et la concentration de chlorophylle, d'azote et de protéines solubles. Ils ont aussi déterminé les effets de l'hormone sur l'activité de la peroxydase et la teneur en polyamines. L'application d'une solution de mT à 0,25, à 0,5 ou à 1,0 mM, respectivement, accélère la croissance des cotylédons de 44, de 50 et de 57 %, comparativement à celle des cotylédons témoins. La plus forte hausse de la concentration de chlorophylle survient aux concentrations de mT le plus faibles. La peroxydase, en revanche, est la plus active avec la solution de mT à 1,0 mM. L'application de mT réduit la concentration de protéines solubles.

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Section: Resultsmentioning

confidence: 99%

“…Plant . Meta-topolin (mT) has been established as an active aromatic cytokinin by Strnad et al (1997). The present investigation assessed the effects of mT on radish cotyledon growth.…”

mentioning

confidence: 99%

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Growth responses of excised radish cotyledons to meta-topolin

Palavan-Ünsal¹,

Çağ²,

Çetin³

2002

Can. J. Plant Sci.

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“…Polyclonal antibodies against 11α-hydroxyprogesterone-hemisuccinate:BSA were raised in rabbits. The immunization schedule and purification of antibodies was as described previously (Strnad et al, 1997). After the fifth immunization the rabbits were bled and the antisera were precipitated with ammonium sulfate (Harlow and Lane, 1988) and dialyzed for five days.…”

Section: Standardsmentioning

confidence: 99%

Development of an ELISA-based kit for the on-farm determination of progesterone in milk

Simerský¹,

Swaczynová²,

Morris³

et al. 2007

Vet. Med. - Czech

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ABSTRACT:Effective reproductive management is a crucial concern in the dairy industry and missed oestrus and late recognition of unsuccessful insemination can lead to substantial financial loss. Progesterone levels change in a predictable manner with progress of the oestrus cycle and with the onset of pregnancy. We report here the development of a simple ELISA test for the measurement of progesterone levels in bovine milk. The method is based on the use of polyclonal antibodies raised against 11α-hydroxyprogesterone-hemisuccinate and competition for antibody binding sites between free progesterone and horseradish peroxidase-labelled progesterone. Binding is quantified by means of the colour reaction between horseradish peroxidase and 3,3' ,5,5'-tetramethylbenzidine. The method has proved to be a cost effective, sensitive and easy to perform alternative to currently available methods of oestrus and pregnancy detection. The practical feasibility of the method has been proven under dairy farm conditions.

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“…Hydroxylated BA analogues have a metabolism distinct from that of BA. Meta-topolin is a newly isolated and highly active natural growth substance (Strnad et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

High in vitro shoot proliferation in the apple cultivar Jonagold induced by benzyladenine analogues

Magyar-Tábori

Dobránszki

Jámbor-Benczúr

2002

Acta Agronomica Hungarica

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The in vitro shoot multiplication of apple cv. Jonagold was tested on media containing benzyladenine, benzyladenine riboside or meta-topolin in different concentrations (from 0.0 to 5.0 mg l -1 ). The optimal concentration for the best multiplication varied according to the type of cytokinin. The highest multiplication rate (on average 6.9 and 5.9 new shoots per explant) was achieved using 5.0 mg l -1 metatopolin or 2.0 mg l -1 benzyladenine riboside. The longest shoots were formed on media containing benzyladenine riboside at a concentration of 0.5 mg l -1 . The length of newly developed shoots was strongly suppressed by high concentrations of different cytokinins, but the suppression effect of a high concentration of meta-topolin on shoot length was less than that of benzyladenine or benzyladenine riboside. In this study meta-topolin and benzyladenine riboside proved to be effective cytokinins to induce adequate shoot proliferation, while benzyladenine was the least active cytokinin.

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Meta-topolin, a highly active aromatic cytokinin from poplar leaves (Populus × canadensis Moench., cv. Robusta)

Cited by 126 publications

References 19 publications

Growth responses of excised radish cotyledons to meta-topolin

Growth responses of excised radish cotyledons to meta-topolin

Development of an ELISA-based kit for the on-farm determination of progesterone in milk

High in vitro shoot proliferation in the apple cultivar Jonagold induced by benzyladenine analogues

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